Carrier graphs for representations of the rank two free group into isometries of hyperbolic three space

Carrier graphs were first introduced for closed hyperbolic 3-manifolds by White. In this paper, we first generalize this definition to carrier graphs for representations of a rank two free group into the isometry group of hyperbolic three space. Then we prove the existence and the finiteness of minimal carrier graphs for those representations which are discrete, faithful and geometrically finite, and more generally, those that satisfy certain finiteness conditions first introduced by Bowditch.Comment: 50 pages, 32 figure

Hsa_circ_0026344 suppresses gastric cancer progression via modulating the miR-1290/FBP2 axis

Background. Circular RNAs (circRNAs) are a novel type of noncoding RNAs and play important roles in tumorigenesis, including gastric cancer (GC). However, the functions of most circRNAs remain poorly understood. In our study, we mainly learn the influence of hsa_circ_0026344 (circ_0026344) in GC progression. Methods. Circ_0026344, miR-1290 and Fructose1,6-bisphosphatase 2 (FBP2) expression was determined by quantitative real-time polymerase chain reaction (qRT-PCR). GC cell proliferation, migration, and invasion were detected by colony formation, 5-ethynyl2’-deoxyuridine (EdU), and transwell assays, respectively. The interaction between circ_0026344 and miR-1290 complex was evaluated by RNA pull-down assay. The interaction of miR-1290 with circ_0026344 or FBP2 was detected using dual-luciferase reporter assay. A xenograft model was established to determine the effect of circ_0026344 on GC tumor growth in vivo. Results. Circ_0026344 expression was dramatically decreased in GC cells and tissues. Circ_0026344 overexpression inhibited GC cell proliferation, migration and invasion. MiR-1290 was predicted as a target of circ_0026344 and miR-1290 overexpression attenuated the anti-tumor effect of circ_0026344 on GC cells. Furthermore, we predicted FBP2 as the target of miR1290. FBP2 knockdown reversed the effects of circ_0026344 knockdown on GC cell malignant behaviors. Functional analysis showed that circ_0026344 upregulated FBP2 expression via miR1290. Additionally, in vivo studies demonstrated that circ_0026344 suppressed GC tumor progression. Conclusion. In conclusion, circ_0026344 inhibited GC cell proliferation via the miR-1290/FBP2 axis, which might provide a new therapeutic target for GC patients

Research on Flow Characteristics of Electronically Controlled Injection Device Developed for High-Power Natural Gas Engines

Accurate fuel supply is a key factor that influences the performance of high-power natural gas engines. The premixed and single-point natural gas supply system is the most commonly used method to ensure a large fuel supply but one of its shortcomings is the inaccuracy of the fuel supply. A new type of natural gas injection device with fungiform configuration and electronically controlled actuator was developed to achieve high efficiency and stable operation in high-power natural gas engines. Firstly, a computational fluid dynamics (CFD) model of the injection device was created. Based on this model, the key structure parameters that have a significant influence on the outlet flow were confirmed. A particle swarm optimization (PSO) model was developed to identify the optimal outflow structure. Then, a flow function for precise flow supply control was constructed based on a response surface model, according to the flow rates of the device under different control parameters. Finally, a flow-characteristic test bench and a high-power engine prototype were developed to verify the simulation and optimization results. The results indicate that the optimized outflow structure shows low pressure loss and a large flow rate, improving injection efficiency by 10.37% and mass flow by 11.78% under 0.4 Mpa pressure difference. More importantly, the cycle fuel supply could be controlled accurately for each cylinder owing to the developed flow function. Consequently, compared with the original engine using a single-point natural gas supply system, the cylinder performance imbalance was improved by 37.47%

Reactivity of nitric oxide with the [4Fe-4S] cluster of dihydroxyacid dehydratase from Escherichia coli

Although the NO (nitric oxide)-mediated modification of iron-sulfur proteins has been well-documented in bacteria and mammalian cells, specific reactivity of NO with iron-sulfur proteins still remains elusive. In the present study, we report the first kinetic characterization of the reaction between NO and iron-sulfur clusters in protein using the Escherichia coli IlvD (dihydroxyacid dehydratase) [4Fe-4S] cluster as an example. Combining a sensitive NO electrode with EPR (electron paramagnetic resonance) spectroscopy and an enzyme activity assay, we demonstrate that NO is rapidly consumed by the IlvD [4Fe-4S] cluster with the concomitant formation of the IlvD-bound DNIC (dinitrosyl-iron complex) and inactivation of the enzyme activity under anaerobic conditions. The rate constant for the initial reaction betweenNOand the IlvD [4Fe-4S] cluster is estimated to be (7.0±2.0)×106 M-2 · s-1 at 25°C, which is approx. 2-3 times faster than that of the NO autoxidation by O2 in aqueous solution. Addition of GSH failed to prevent the NOmediated modification of the IlvD [4Fe-4S] cluster regardless of the presence of O2 in the medium, further suggesting that NO is more reactivewith the IlvD [4Fe-4S] cluster than with GSH or O2. Purified aconitase B [4Fe-4S] cluster from E. coli has an almost identical NO reactivity as the IlvD [4Fe-4S] cluster. However, the reaction between NO and the endonuclease III [4Fe-4S] cluster is relatively slow, apparently because the [4Fe-4S] cluster in endonuclease III is less accessible to solvent than those in IlvD and aconitase B. When E. coli cells containing recombinant IlvD, aconitase B or endonuclease III are exposed to NO using the Silastic tubing NO delivery system under aerobic and anaerobic conditions, the [4Fe-4S] clusters in IlvD and aconitase B, but not in endonuclease III, are efficiently modified forming the proteinbound DNICs, confirming that NO has a higher reactivity with the [4Fe-4S] clusters in IlvD and aconitase B than with O2 or GSH. The results suggest that the iron-sulfur clusters in proteins such as IlvD and aconitase B may constitute the primary targets of the NO cytotoxicity under both aerobic and anaerobic conditions. © The Authors Journal compilation © 2009 Biochemical Society

Poly (3-methylthiophene) based electrochemical actuators

Polythiophene, one of the most extensively studied conducting polymers, was selected as an actuator material due to its chemical and electrochemical stability both in air and moisture. In this work, poly(3-methylthiophene) based actuators were constructed electrochemically with a tubular geometrical configuration. The actuation behaviour was investigated regarding to the actuation strain generated, the stress produced and work per cycle performed by poly(3-methylthiophene) actuators. The effect of potential sweep rate and different electrolytes (ionic liquid and organic solvent) on the actuation performance were also explored. Poly(3-methylthiophene) actuators show an increase in actuation strain with an increase in applied load

Influence of Prewhirl Regulation by Inlet Guide Vanes on Cavitation Performance of a Centrifugal Pump

The influence of prewhirl regulation by inlet guide vanes (IGVs) on a centrifugal pump performance is investigated experimentally and numerically. The experimental results show that IGVs can obviously change the head and increase the efficiency of the tested centrifugal pump over a wide range of flow rates. Although the cavitation performance is degraded, the variation of the cavitation critical point is less than 0.5 m. Movement of the computed three-dimensional streamlines in suction pipe and impeller are analyzed in order to reveal the mechanism how the IGVs realize the prewhirl regulation. The calculated results show that the influence of IGVs on the cavitation performance of centrifugal pump is limited by a maximum total pressure drop of 1777 Pa, about 7.6% of the total pressure at the suction pipe inlet for a prewhirl angle of 24°

SEPTIN2 suppresses an IFN-γ-independent, proinflammatory macrophage activation pathway

Interferon-gamma (IFN-γ) signaling is necessary for the proinflammatory activation of macrophages but IFN-γ-independent pathways, for which the initiating stimuli and downstream mechanisms are lesser known, also contribute. Here we identify, by high-content screening, SEPTIN2 (SEPT2) as a negative regulation of IFN-γ-independent macrophage autoactivation. Mechanistically, endoplasmic reticulum (ER) stress induces the expression of SEPT2, which balances the competition between acetylation and ubiquitination of heat shock protein 5 at position Lysine 327, thereby alleviating ER stress and constraining M1-like polarization and proinflammatory cytokine release. Disruption of this negative feedback regulation leads to the accumulation of unfolded proteins, resulting in accelerated M1-like polarization, excessive inflammation and tissue damage. Our study thus uncovers an IFN-γ-independent macrophage proinflammatory autoactivation pathway and suggests that SEPT2 may play a role in the prevention or resolution of inflammation during infection

Low-mass dark matter search results from full exposure of PandaX-I experiment

We report the results of a weakly-interacting massive particle (WIMP) dark matter search using the full 80.1\;live-day exposure of the first stage of the PandaX experiment (PandaX-I) located in the China Jin-Ping Underground Laboratory. The PandaX-I detector has been optimized for detecting low-mass WIMPs, achieving a photon detection efficiency of 9.6\%. With a fiducial liquid xenon target mass of 54.0\,kg, no significant excess event were found above the expected background. A profile likelihood analysis confirms our earlier finding that the PandaX-I data disfavor all positive low-mass WIMP signals reported in the literature under standard assumptions. A stringent bound on the low mass WIMP is set at WIMP mass below 10\,GeV/c$^2$, demonstrating that liquid xenon detectors can be competitive for low-mass WIMP searches.Comment: v3 as accepted by PRD. Minor update in the text in response to referee comments. Separating Fig. 11(a) and (b) into Fig. 11 and Fig. 12. Legend tweak in Fig. 9(b) and 9(c) as suggested by referee, as well as a missing legend for CRESST-II legend in Fig. 12 (now Fig. 13). Same version as submitted to PR