Transfection of Neuroprogenitor Cells with Iron Nanoparticles for Magnetic Resonance Imaging Tracking: Cell Viability, Differentiation, and Intracellular Localization

Magnetic resonance imaging (MRI) can track labeled cells in the brain. The use of hemagglutinating virus of Japan envelopes (HVJ-Es) to effectively introduce the contrast agent to neural progenitor cells (NPCs) is limited to date despite their high NPC affinity.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/41579/1/11307_2005_Article_8.pd

The role of 2-deoxy-2-[F-18]fluoro-D-glucose positron emission tomography in disseminated carcinoma of unknown primary site

articleInternational audienc

T-Cell Homing to the Pancreas in Autoimmune Mouse Models of Diabetes: In Vivo MR Imaging

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Fluorodeoxyglucose positron emission tomography for the diagnosis of sarcoidosis in patients with unexplained chronic uveitis

articleInternational audienc

Nanosystems for medical applications: Biological detection, drug delivery, diagnosis and therapy

National audienceA review. For a couple of decades, greater and greater connections have been made between nanotechnology, biology and medicine. After a rapid description of the particles most often used for biological and medical purposes, the review will detail their potential applications in both domains. In the field of biological detection, a large number of new detection systems is offered by noble metals and semi-conductors, which exhibit very specific nanometre-scale induced properties. In the field of diagnosis and therapeutic applications, particles become more and more sophisticated with an increased possibility of specific targeting, drug delivery triggering and combination of both diagnosis and therapy

Malignant mesothelioma of the peritoneum as the cause of a paraneoplastic syndrome: detection by 18F-FDG PET

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Gadolinium-based nanoparticles sensitize head and neck carcinoma stem and nonstem cells to low and high LET radiation

International audienceHead and neck squamous cell carcinoma (HNSCC) is an aggressive and recurrent malignancy owing to intrinsic radioresistance and lack of induction of apoptosis. Several strategies aiming at radiosensitizing these tumors are currently being developed, one of these relying on the use of nanoparticles including high Z elements such as gadolinium (Z=64). Once delivered to the tumor, gadolinium-based nanoparticles (GBNs) should amplify the efficacy of radiotherapy through the generation of electron Auger cascades and secondary electrons. This study aims at demonstrating the in vitro and in vivo radiosensitizing effect of sub-5 nm GBNs (composed of a core of gadolinium oxide, a shell of polysiloxane, and functionalized by diethylene triaminepentaacetic acid) in a SQ20B radioresistant HNSCC model. Owing to the crucial role of cancer stem cells in tumor initiation, disease recurrence and radioresistance, the radiosensitizing effect of GBNs was also tested in SQ20B stem-like cells. The association of 0,6 mM GBNs with a photon or a 33.6 keV/µm carbon ion irradiation decrease significantly SQ20B cell survival. Radiosensitization goes through the increase in non-reparable DNA double-strand breaks, the shortening of G2/M phase blockage, the inhibition of cell proliferation, each contributing to the commitment into apoptosis. The combined treatment of GBNs with irradiation can also overcome the resistance of SQ20B stem-like cells. Using an SQ20B tumor-bearing mouse model, we also demonstrate that GBNs in conjunction with photon irradiation significantly retard tumor growth compared with the radiation alone, with complete remission in some mice. Significantly, an increase in apoptosis and decrease in cell proliferation are also detected inside tumors in the combined treatment group. These results suggest the interesting potential of GBNs in sensitizing resistant HNSCC tumors to radiotherapy and their possible contribution towards overcoming limitations of current cancer treatment. This project was conducted under the framework of LANTHARAD (PDC019 CLARA 2010), PRRH-ETOILE and LABEX PRIMES (2012