MALDI-TOF MS contribution to diagnosis of melioidosis in a nonendemic country in three French travellers

Melioidosis is an endemic disease in Southeast Asia and northern Australia. An increasing number of cases are being reported in nonendemic countries, making the diagnosis less obvious. We discuss the identification of Burkholderia pseudomallei using matrix-assisted desorption ionization–time of flight mass spectrometry on the occasion of recent cases of imported melioidosis in French travellers

The growing chaos of tuberculosis population genomics at the era of 'Big Data’: sorting out the wheat from the chaff

International audience<div&gtThe publication of a couple of recent landmark papers (Freschi et al. 2021, Napier et al. 2021, Coscolla et al. 2021, Thawornwattana et al. 2021) claiming the discovery of new WGS-defined clades, prompted us to reevaluate both the SNP informativity and the hierarchical naming of some of the phylogenetical structures described in these articles. Thanks to a new proprietary informatical platform, TB-ANNOTATOR, we performed a benchmark analysis of these articles, and present results that allow to create new links between the pre-genomic and the post-genomic era for young researchers entering into the field, reassessing the SNP informativity, the link between polymorhic markers, and showing current discrepancies between studies, suggesting that even in large databases, the global population structure of &nbsp;MTBC remains strongly dependent on sample origin, WGS quality and bioinformatical tools. We also describe some recent improvements in phylogenetical analysis of MTBC.</div&gt<div&gt<br&gt</div&g

Characterization of a P1-like bacteriophage encoding an SHV-2 extended-spectrum β-lactamase from an Escherichia coli strain.

International audienc

Pneumonia-Specific Escherichia coli with Distinct Phylogenetic and Virulence Profiles, France, 2012–2014

International audienceIn a prospective, nationwide study in France of Escherichia coli responsible for pneumonia in patients receiving mechanical ventilation, we determined E. coli antimicrobial susceptibility, phylotype, O-type, and virulence factor gene content. We compared 260 isolates with those of 2 published collections containing commensal and bacteremia isolates. The preponderant phylogenetic group was B2 (59.6%), and the predominant sequence type complex (STc) was STc73. STc127 and STc141 were overrepresented and STc95 underrepresented in pneumonia isolates compared with bacteremia isolates. Pneumonia isolates carried higher proportions of virulence genes sfa/foc, papGIII, hlyC, cnf1, and iroN compared with bacteremia isolates. Virulence factor gene content and antimicrobial drug resistance were higher in pneumonia than in commensal isolates. Genomic and phylogenetic characteristics of E. coli pneumonia isolates from critically ill patients indicate that they belong to the extraintestinal pathogenic E. coli pathovar but have distinguishable lung-specific traits