New Complex Chromosomal Translocation in Chronic Myeloid Leukaemia: t(9;18;22)(q34;p11;q11)

A Chronic myeloid leukaemia (CML) case with a new complex t(9;18;22)(q34;p11;q11) of a 29-year-old man is being reported. For the first time, this translocation has been characterized by karyotype complemented with fluorescence in situ hybridization (FISH). In CML, the complex and standard translocations have the same prognosis. The patient was treated with standard initial therapy based on hydroxyurea before he died due to heart failure four months later. Our finding indicates the importance of combined cytogenetic analysis for diagnosis and guidance of treatment in clinical diagnosis of CML

CDKN1B (cyclin-dependent kinase inhibitor 1 B )

Review on CDKN1B (cyclin-dependent kinase inhibitor 1 B ), with data on DNA, on the protein encoded, and where the gene is implicated

t(8;14)(q24;q32) - t(2;8)(p12;q24) - t(8;22)(q24;q11)

Review on t(8;14)(q24;q32) - t(2;8)(p12;q24) - t(8;22)(q24;q11), with data on clinics, and the genes involved

12p abnormalities in myeloid malignancies

Review on 12p abnormalities in myeloid malignancies, with data on clinics, and the genes involved

i(17q) in myeloid malignancies

Review on i(17q) in myeloid malignancies, with data on clinics, and the genes involved

t(11;14)(p13;q11) - t(7;11)(q35;p13)

Review on t(11;14)(p13;q11) - t(7;11)(q35;p13), with data on clinics, and the genes involved

del(6q) abnormalities in lymphoid malignancies

Review on del(6q) abnormalities in lymphoid malignancies, with data on clinics, and the genes involved

Enzymatic reactor using high activity immobilized enzymes during exothermic reaction

International audienceThe exothermic reaction used is the decomposition of hydrogen peroxyde into oxygen and water, the enthalphy of which reaches -23.44 kcal.mole-1 at 25°C and under 1 atmosphere for the pure liquid. An advantage of using insolubilized enzyme reactors lies in the saving of energy because enzymes allow work at ambient temperature. Nevertheless, some exothermic reactions may lead to a progressive denaturation of the enzyme. The study of the reactor using catalase for hydrogen peroxide decomposition is conducted here

Ontogenic Changes in Hematopoietic Hierarchy Determine Pediatric Specificity and Disease Phenotype in Fusion Oncogene-Driven Myeloid Leukemia.

Fusion oncogenes are prevalent in several pediatric cancers, yet little is known about the specific associations between age and phenotype. We observed that fusion oncogenes, such as ETO2-GLIS2, are associated with acute megakaryoblastic or other myeloid leukemia subtypes in an age-dependent manner. Analysis of a novel inducible transgenic mouse model showed that ETO2-GLIS2 expression in fetal hematopoietic stem cells induced rapid megakaryoblastic leukemia whereas expression in adult bone marrow hematopoietic stem cells resulted in a shift toward myeloid transformation with a strikingly delayed in vivo leukemogenic potential. Chromatin accessibility and single-cell transcriptome analyses indicate ontogeny-dependent intrinsic and ETO2-GLIS2-induced differences in the activities of key transcription factors, including ERG, SPI1, GATA1, and CEBPA. Importantly, switching off the fusion oncogene restored terminal differentiation of the leukemic blasts. Together, these data show that aggressiveness and phenotypes in pediatric acute myeloid leukemia result from an ontogeny-related differential susceptibility to transformation by fusion oncogenes. SIGNIFICANCE: This work demonstrates that the clinical phenotype of pediatric acute myeloid leukemia is determined by ontogeny-dependent susceptibility for transformation by oncogenic fusion genes. The phenotype is maintained by potentially reversible alteration of key transcription factors, indicating that targeting of the fusions may overcome the differentiation blockage and revert the leukemic state.See related commentary by Cruz Hernandez and Vyas, p. 1653.This article is highlighted in the In This Issue feature, p. 1631