Genetic factors of hypertension in individuals with different rates of Na-Li counter -transport

Currently, hypertension is recognized as one of the most common cardio-vascular diseases. Recent studies indicate the multifactorial nature of this disease, in which much emphasis is given to genetic factors for their role in the development of this disease. With, results based on the identification of genetic markers associated with the risk of developing hypertension vary vastly and remain highly controversial, primarily due to the ethno-genetic and geographical features of the populations under study, there is an urgent need to identify a phenotypic trait that would allow analysis of associations with the risk of developing hypertension irrespective of an individual's ethnic background or geographical location. One such a prospective trait is the rate of Na-Li counter-transport, which remains unchanged for most of an individual's lifetime, with rare exceptions. Hence this study is aimed at identifying the contribution of gene polymorphisms of the renin-angiotensin system in the formation of genetic predisposition to hypertension in individuals with different rates of erythrocyte Na-Li counter-transport

Genetic factors of hypertension in individuals with different rates of Na-Li counter -transport

Currently, hypertension is recognized as one of the most common cardio-vascular diseases. Recent studies indicate the multifactorial nature of this disease, in which much emphasis is given to genetic factors for their role in the development of this disease. With, results based on the identification of genetic markers associated with the risk of developing hypertension vary vastly and remain highly controversial, primarily due to the ethno-genetic and geographical features of the populations under study, there is an urgent need to identify a phenotypic trait that would allow analysis of associations with the risk of developing hypertension irrespective of an individual's ethnic background or geographical location. One such a prospective trait is the rate of Na-Li counter-transport, which remains unchanged for most of an individual's lifetime, with rare exceptions. Hence this study is aimed at identifying the contribution of gene polymorphisms of the renin-angiotensin system in the formation of genetic predisposition to hypertension in individuals with different rates of erythrocyte Na-Li counter-transport

Biochemical & immunological markers of autoimmune thyroiditis

Correlations between biochemical and immunological markers of programmed cell death (apoptosis), and the functional state of the thyroid gland (hyperthyroidism, euthyroidism, hypothyroidism) have been investigated in autoimmune thyroiditis (AT) (also known as chronic autoimmune thyroiditis). Annexin V, TRAIL and TNF-α, as well as DNA-hydrolyzing antibodies were used as the main markers. Increased levels of TRAIL were found in the serum of AT patients (hyperthyroidism>hypothyroidism>euthyroidism) compared with healthy individuals. The highest frequency of antibodies to denatured DNA (Abs-dDNA) had the highest frequency in AT patients (97%) compared with healthy controls. Among these patients, 75% had hyperthyroidism, 85% had hypothyroidism, and 84.7% had euthyroidism. Abs hydrolyzing activity demonstrated correlation dependence with symptoms of the thyroid dysfunction

Biochemical and immunological markers of autoimmune thyroiditis

© 2015, Pleiades Publishing, Ltd. Correlations between biochemical and immunological markers of programmed cell death (apoptosis), and the functional state of the thyroid gland (hyperthyroidism, euthyroidism, hypothyroidism) have been investigated in autoimmune thyroiditis (AT) (also known as chronic autoimmune thyroiditis). Annexin V, TRAIL and TNFα, as well as DNA-hydrolyzing antibodies were used as the main markers. Increased levels of TRAIL were found in the serum of AT patients (hyperthyroidism > hypothyroidism > euthyroidism) compared with healthy individuals. The highest frequency of antibodies to denatured DNA (Abs-dDNA) had the highest frequency in AT patients (97%) compared with healthy controls. Among these patients, 75% had hyperthyroidism, 85% had hypothyroidism, and 84.7% had euthyroidism. Abs hydrolyzing activity demonstrated correlation dependence with symptoms of the thyroid dysfunction

Detection of macro-thyrotropinaemia in patients with Hashimotos thyroiditis and subclinical hypothyroidism

The level of thyroid stimulating hormone is one of the diagnostic indicators of thyroid function. In subclinical hypothyroidism, its concentration in the blood serum increases, while the level of thyroid hormones remains normal. One of the reasons for this is the phenomenon of macrotyrotropinemia, in which the macro isoforms of thyrotropin (a complex of thyrotropic hormone with immunoglobulin) are present in the blood. It is assumed that the biological activity of macrotyrotropin is low, and may accumulate in the circulation, causing a falsely elevated level of thyroid-stimulating hormone in serum. The aim of this study is to identify the nature and prevalence of the macrothyrotropinemia phenomenon among patients with subclinical hypothyroidism in presence of autoimmune thyroiditis and a group of healthy donors. Materials and methods: Fifty serum samples of venous blood served as the material for the study: 30 patients with subclinical hypothyroidism in presence of autoimmune thyroiditis; 10, with manifesting hypothyroidism, 10 conditionally healthy donors without thyroid gland pathology (control group). The group was derived from results of the clinical laboratory at the Clinical Hospital at the Kazan station railway. Patients’ blood serum was screened for the presence of macrotrorotropin by polyethylene glycol precipitation method, followed by analysis by gel filtration chromatography. Results of this study were as follows: screening of blood sera was performed by gel filtration and affinity chromatography. Polyethylene glycol was shown to precipitate 50 to 100% serum thyrotropin, of which true macrotrothropin makes 56-98%. In the patients with subclinical hypothyroidism with a thyroid-stimulating hormone level of more than 10 pIU/ml, a trend towards an increase in the level of macrothyrotrophinaemia has been shown. The content of macrotyrotropin complex in patients with subclinical hypothyroidism, in whom the level of antibodies to thyroperoxidase is > 500 U/L, is significantly higher if compared to the patients with manifesting hypothyroidism. Elevated levels of antibodies to thyroperoxidase can lead to the generation of macrotyropin. Our findings have shown that the phenomenon of macrothyrotropinemia is quite common in patients with subclinical and manifesting hypothyroidism with Hashimoto thyroiditis (53.3%) and in control group (25%). Macrotyrotropin complex probably consists of thyrotropin and IgG. Patients with a thyroid-stimulating hormone level of > 10 pIU/ml are candidates for screening for the presence of the macrotyrotropin complex.The activity of the autoimmune process may correlate with the phenomenon of macrothyrotropinemia. The results can be used to develop an additional tool when choosing therapy in clinical practice

Le Petit Troyen : journal démocratique régional ["puis" journal quotidien de la démocratie de l'Est "puis" grand quotidien de la Champagne]

17 juin 19431943/06/17 (A65,N22468).Appartient à l’ensemble documentaire : ChArdenn

Genetic evaluation and selective breeding of Oreochromis niloticus for broodstock development : final report, phase I (10 June 1986 – 9 June 1989)

Relevance of the problem stated in the article is related to selecting and mastering the use of new methods of teaching reading in English to Russian students. The article aims to identify new ways of developing reading skills of the students at the senior stage of learning the English language. The leading methods are analysis of scientific works, empirical and experimental data, method of involved observation, modeling and experimental method. The experimental method is aimed to search new means of teaching reading to higher school students. The article presents an attempt to use the text materials from newspapers and magazines in English for improving reading skills with understanding of the basic content of the text. The experiment has been conducted on the basis of Grade 9 of the secondary school in Kazan (Russia). In it two groups of 13 people participated. On comparing the percentage of the quality of the first lesson of the experiment (34 %) and the last one (96 %) it can be concluded that the students have achieved good results in reading with understanding of the core content of the texts from newspapers and magazines, despite the fact that they did not own these skills before

New approaches to teaching reading English texts at the senior stage of education

Relevance of the problem stated in the article is related to selecting and mastering the use of new methods of teaching reading in English to Russian students. The article aims to identify new ways of developing reading skills of the students at the senior stage of learning the English language. The leading methods are analysis of scientific works, empirical and experimental data, method of involved observation, modeling and experimental method. The experimental method is aimed to search new means of teaching reading to higher school students. The article presents an attempt to use the text materials from newspapers and magazines in English for improving reading skills with understanding of the basic content of the text. The experiment has been conducted on the basis of Grade 9 of the secondary school in Kazan (Russia). In it two groups of 13 people participated. On comparing the percentage of the quality of the first lesson of the experiment (34 %) and the last one (96 %) it can be concluded that the students have achieved good results in reading with understanding of the core content of the texts from newspapers and magazines, despite the fact that they did not own these skills before

Biochemical & immunological markers of autoimmune thyroiditis

Correlations between biochemical and immunological markers of programmed cell death (apoptosis), and the functional state of the thyroid gland (hyperthyroidism, euthyroidism, hypothyroidism) have been investigated in autoimmune thyroiditis (AT) (also known as chronic autoimmune thyroiditis). Annexin V, TRAIL and TNF-α, as well as DNA-hydrolyzing antibodies were used as the main markers. Increased levels of TRAIL were found in the serum of AT patients (hyperthyroidism>hypothyroidism>euthyroidism) compared with healthy individuals. The highest frequency of antibodies to denatured DNA (Abs-dDNA) had the highest frequency in AT patients (97%) compared with healthy controls. Among these patients, 75% had hyperthyroidism, 85% had hypothyroidism, and 84.7% had euthyroidism. Abs hydrolyzing activity demonstrated correlation dependence with symptoms of the thyroid dysfunction

ASSOCIATION OF IL-1β, IL-4 AND IL-6 GENES POLYMORPHISMS WITH GENETIC PREDISPOSITION FOR AUTOIMMUNE THYROIDITIS

Abstract.  Autoimmune  thyroiditis  (Hashimoto’s  thyroiditis,  HT)  still  represents  an  unresolved  problem of modern endocrinology, since its etiology and pathogenesis are yet unclear. Cytokines play an important role in the regulation of immune and inflammatory response, therefore, some gene variants encoding them, are considered as potential risk risk factors for autoimmune thyroid diseases. We have genotyped 298 women from the Republic of Tatarstan (RT, control group, 137 persons; HT patients, 161) for the following gene polymorphisms: interleukin-1 beta (IL-1β) +3953 C/T (rs 1143634); interleukin 4 (IL-4) -590C/T (rs 2243250), and interleukin 6 (IL-6) -174C/G (rs 1800795), using allele-specific PCR. The results showed a significantly increased frequencies of C allele (P  = 0.0003) and CT genotype (P  = 0.048, OR  = 6.05, 95%CI 2.59-2.97) of  +3953  C/T  of  IL-1β  in  patients  with  Hashimoto's thyroiditis, as compared with control group. The latter  group showed higher prevalence of T allele (P  = 0.0003) and TT genotype (P  = 4.95 Ч 6.10; OR  = 0.15, 95%CI 0.06-0.38). The 590C/T and -174C/G polymorphic variants in, resp., IL-4 and IL-6 genes are not associated with increased risk of this disease among women from RT. (Med. Immunol., 2011, vol. 13, N 6, pp 603-608