Vaginal lipidomics of women with vulvovaginal candidiasis and cytolytic vaginosis: a non-targeted LC-MS pilot study

sem informaçãoTo characterize the lipid profile in vaginal discharge of women with vulvovaginal candidiasis, cytolytic vaginosis, or no vaginal infection or dysbiosis. Design. Cross-sectional study. Setting. Genital Infections Ambulatory, Department of Tocogynecology,138sem informaçãosem informaçãosem informaçãoThe supervision of the laboratory studies by Gustavo Henrique Bueno Duarte at Thomson Mass Spectrometry Laboratory–Campinas State Universit

Melhorar a qualidade de modelo de distribuição das espécies com um algoritmo paralelo de programação linear genético-fuzzy.

Biodiversity, the variety of life on the planet, is declining due to climate change, population and species interactions and as the result f demographic and landscape dynamics. Integrated model-based assessments play a key role in understanding and exploring these complex dynamics and have proven use in conservation planning. Model-based assessments using Species Distribution Models constitute an efficient means of translating limited point data to distribution probability maps for current and future scenarios in support of conservation decision making. The aims of this doctoral study were to investigate; (1) the use of a hybrid genetic programming to build high quality models that handle noisy real-world presence and absence data, (2) the extension of this solution to exploit the parallelism inherent to genetic programming for fast scenario based decision making tasks, and (3) a conceptual framework to share models in the hope of enabling research synthesis. Subsequent to this, the quality of the method, evaluated with the true skill statistic, was examined with two case studies. The first with a dataset obtained by defining a virtual species, and the second with data extracted from the North American Breeding Bird Survey relating to mourning dove (Zenaida macroura). In these studies, the produced models effectively predicted the species distribution up to 30% of error rate both presence and absence samples. The parallel implementation based on a twenty-node c3.xlarge Amazon EC2 StarCluster showed a linear speedup due to the multiple-deme coarse-grained design. The hybrid fuzzy genetic programming algorithm generated under certain consitions during the case studies significantly better transferable models.Biodiversidade, a variedade de vida no planeta, está em declínio às alterações climáticas, mudanças nas interações das populações e espécies, bem como nas alterações demográficas e na dinâmica de paisagens. Avaliações integradas baseadas em modelo desempenham um papel fundamental na compreensão e na exploração destas dinâmicas complexas e tem o seu uso comprovado no planejamento de conservação da biodiversidade. Os objetivos deste estudo de doutorado foram investigar; (1) o uso de técnicas de programação genética e fuzzy para construir modelos de alta qualidade que lida com presença e ausência de dados ruidosos do mundo real, (2) a extensão desta solução para explorar o paralelismo inerente à programação genética para acelerar tomadas de decisão e (3) um framework conceitual para compartilhar modelos, na expectativa de permitir a síntese de pesquisa. Subsequentemente, a qualidade do método, avaliada com a true skill statistic, foi examinado com dois estudos de caso. O primeiro utilizou um conjunto de dados fictícios obtidos a partir da definição de uma espécie virtual, e o segundo utilizou dados de uma espécie de pomba (Zenaida macroura) obtidos do North American Breeding Bird Survey. Nestes estudos, os modelos foram capazes de predizer a distribuição das espécies maneira correta mesmo utilizando bases de dados com até 30% de erros nas amostras de presença e de ausência. A implementação paralela utilizando um cluster de vinte nós c3.xlarge Amazon EC2 StarCluster, mostrou uma aceleração linear devido ao arquitetura de múltiplos deme de granulação grossa. O algoritmo de programação genética e fuzzy gerada em determinadas condições durante os estudos de caso, foram significativamente melhores na transferência do que os algoritmos do BIOMOD

Vaginal lipidomics of women with vulvovaginal candidiasis and cytolytic vaginosis: A non-targeted LC-MS pilot study.

OBJECTIVE:To characterize the lipid profile in vaginal discharge of women with vulvovaginal candidiasis, cytolytic vaginosis, or no vaginal infection or dysbiosis. DESIGN:Cross-sectional study. SETTING:Genital Infections Ambulatory, Department of Tocogynecology, University of Campinas, Campinas, São Paulo-Brazil. SAMPLE:Twenty-four women were included in this study: eight with vulvovaginal candidiasis, eight with cytolytic vaginosis and eight with no vaginal infections or dysbiosis (control group). METHODS:The lipid profile in vaginal discharge of the different study groups was determined by liquid chromatography-mass spectrometry and further analyzed with MetaboAnalyst 3.0 platform. MAIN OUTCOME MEASURES:Vaginal lipids concentration and its correlation with vulvovaginal candidiasis and cytolytic vaginosis. RESULTS:PCA, PLS-DA and hierarchical clustering analyses indicated 38 potential lipid biomarkers for the different groups, correlating with oxidative stress, inflammation, apoptosis and integrity of the vaginal epithelial tissue. Among these, greater concentrations were found for Glycochenodeoxycholic acid-7-sulfate, O-adipoylcarnitine, 1-eicosyl-2-heptadecanoyl-glycero-3-phosphoserine, undecanoic acid, formyl dodecanoate and lipoic acid in the vulvovaginal candidiasis group; N-(tetradecanoyl)-sphinganine, DL-PPMP, 1-oleoyl-cyclic phosphatidic, palmitic acid and 5-aminopentanoic acid in the cytolytic vaginosis group; and 1-nonadecanoyl-glycero-3-phosphate, eicosadienoic acid, 1-stearoyl-cyclic-phosphatidic acid, 1-(9Z,12Z-heptadecadienoyl)-glycero-3-phosphate, formyl 9Z-tetradecenoate and 7Z,10Z-hexadecadienoic acid in the control group. CONCLUSIONS:Lipids related to oxidative stress and apoptosis were found in higher concentrations in women with vulvovaginal candidiasis and cytolytic vaginosis, while lipids related to epithelial tissue integrity were more pronounced in the control group. Furthermore, in women with cytolytic vaginosis, we observed higher concentrations of lipids related to bacterial overgrowth