WARTOŚĆ HANDLOWA TUSZ LOSZEK TUCZNIKÓW I LOSZEK PIERWIASTEK

Study comprised 60 crossbred gilts F1 (PLW x PL), 30 fattener gilts (A) and 30 littermate primiparous gilts (B). The fattening was conducted in standard condition from 30 to 108 kg body weight. The primiparous gilts were mated at second oestrus. During pregnancy and lactation period they were reared in standard condition adopted for pregnant and lactating sows. Fattener sows as well as primiparous sows were slaughtered after end of the experiment. Market carcass value was estimated on carcass jointing basis. It was assumed that market value is a function of percentage share of main parts in carcass and their unitary price (PLN/kg). Market carcass value of fattener sows and primiparous sows counted on 100 kg was comparable.Badaniami objęto 60 loszek, mieszańców F1 rasy polskiej białej zwisłouchej i wielkiej białej polskiej, w tym 30 loszek tuczników (A) i 30 loszek pierwiastek ubijanych po odchowaniu pierwszego miotu. Tucz prowadzono w warunkach chowu tradycyjnego od masy ciała 30 kg do około 107 kg. Loszki pierwiastki zostały pokryte w drugiej rui. W okresie ciąży i po oproszeniu utrzymywane były w warunkach standardowych dla loch prośnych i karmiących. Zarówno loszki tuczniki jak i loszki pierwiastki zostały poddane ubojowi oraz ocenie jakości tusz. Wyliczono udział procentowy poszczególnych elementów rozbioru w tuszy. Przy ocenie wartości handlowej tusz przyjęto założenie, że jest ona funkcją masy uzyskanych elementów w odniesieniu do ich procentowego udziału oraz ceny jednostkowej (zł/kg). Wartość handlowa tusz loszek tuczników i loszek pierwiastek w przeliczeniu na 100 kg była porównywalna

WARTOŚĆ RZEŹNA TUSZ ŚWIŃ W ZALEŻNOŚCI OD GENOTYPU KOLIPAZY (CLPS) Z UWZGLĘDNIENIEM ODDZIAŁYWANIA GENU RYR1

The experiment was carried out on 110 3-breed crossbreds fatteners [♂ Pietrain x (♀ Polish Large White x Polish Landrace)]. Pigs were reared in standard conditions and slaughtered at an average body weight of 105 kg. Gene polymorphism was determined with the PCR-RFLP procedure (CLPS/DdeI and RYR1/HinPI). One day postslaughter, the right carcass-sides were dissected and evaluated using the methods applied for the Polish Pig Testing Station (SKURTCh). The study evaluated the slaughter value of carcasses depending on colipase genotype (CLPS) with infl uence of major gene RYR1. Colipase gene differentiated carcass lean content only in RYR1CC pigs and in all pigs the neck weight as well as its tissue composition in RYR1CT pigs. Moreover, signifi cant infl uence of major gene (RYR1T) on quantitative traits was confi rmed.Badaniem objęto 110 tuczników, mieszańców 3-rasowych [♂ Pietrain x (♀Wielka Biała Polska x Polska Biała Zwisłoucha). Zwierzęta utrzymywane były w ujednoliconych warunkach i ubijane przy zbliżonej masie ciała, około 105 kg. Polimorfi zm genów został określony przy użyciu metody PCR-RFLP (CLPS/DdeI i RYR1/HinPI). Dzień po uboju, prawe półtusze poddano dysekcji i ocenie zgodnie z metodyką opracowaną dla polskich Stacji Kontroli Użytkowości Rzeźnej Trzody Chlewnej. Ocenie poddano wartość rzeźną tusz w zależności od genotypu kolipazy (CLPS), uwzględniając przy tym wpływ genu głównego RYR1. Gen kolipazy istotnie różnicował zawartość mięsa w tuszy tylko u tuczników o genotypie RYR1CC oraz u wszystkich świń masę karkówki i jej skład tkankowy u świń RYR1CT. Ponadto, potwierdzono wpływ genu głównego na cechy ilościowe tusz

On the dynamics of the adenylate energy system: homeorhesis vs homeostasis.

Biochemical energy is the fundamental element that maintains both the adequate turnover of the biomolecular structures and the functional metabolic viability of unicellular organisms. The levels of ATP, ADP and AMP reflect roughly the energetic status of the cell, and a precise ratio relating them was proposed by Atkinson as the adenylate energy charge (AEC). Under growth-phase conditions, cells maintain the AEC within narrow physiological values, despite extremely large fluctuations in the adenine nucleotides concentration. Intensive experimental studies have shown that these AEC values are preserved in a wide variety of organisms, both eukaryotes and prokaryotes. Here, to understand some of the functional elements involved in the cellular energy status, we present a computational model conformed by some key essential parts of the adenylate energy system. Specifically, we have considered (I) the main synthesis process of ATP from ADP, (II) the main catalyzed phosphotransfer reaction for interconversion of ATP, ADP and AMP, (III) the enzymatic hydrolysis of ATP yielding ADP, and (IV) the enzymatic hydrolysis of ATP providing AMP. This leads to a dynamic metabolic model (with the form of a delayed differential system) in which the enzymatic rate equations and all the physiological kinetic parameters have been explicitly considered and experimentally tested in vitro. Our central hypothesis is that cells are characterized by changing energy dynamics (homeorhesis). The results show that the AEC presents stable transitions between steady states and periodic oscillations and, in agreement with experimental data these oscillations range within the narrow AEC window. Furthermore, the model shows sustained oscillations in the Gibbs free energy and in the total nucleotide pool. The present study provides a step forward towards the understanding of the fundamental principles and quantitative laws governing the adenylate energy system, which is a fundamental element for unveiling the dynamics of cellular life

CARCASS MARKET VALUE OF FATTENER GILTS AND PRIMIPAROUS GILTS

Study comprised 60 crossbred gilts F1 (PLW x PL), 30 fattener gilts (A) and 30 littermate primiparous gilts (B). The fattening was conducted in standard condition from 30 to 108 kg body weight. The primiparous gilts were mated at second oestrus. During pregnancy and lactation period they were reared in standard condition adopted for pregnant and lactating sows. Fattener sows as well as primiparous sows were slaughtered after end of the experiment. Market carcass value was estimated on carcass jointing basis. It was assumed that market value is a function of percentage share of main parts in carcass and their unitary price (PLN/kg). Market carcass value of fattener sows and primiparous sows counted on 100 kg was comparable

SLAUGHTER VALUE OF PIGS CARCASSES AS RELATED TO COLIPASE GENOTYPE (CLPS) CONSIDERING RYR1 GENE EFFECT

The experiment was carried out on 110 3-breed crossbreds fatteners [♂ Pietrain x (♀ Polish Large White x Polish Landrace)]. Pigs were reared in standard conditions and slaughtered at an average body weight of 105 kg. Gene polymorphism was determined with the PCR-RFLP procedure (CLPS/DdeI and RYR1/HinPI). One day postslaughter, the right carcass-sides were dissected and evaluated using the methods applied for the Polish Pig Testing Station (SKURTCh). The study evaluated the slaughter value of carcasses depending on colipase genotype (CLPS) with infl uence of major gene RYR1. Colipase gene differentiated carcass lean content only in RYR1CC pigs and in all pigs the neck weight as well as its tissue composition in RYR1CT pigs. Moreover, signifi cant infl uence of major gene (RYR1T) on quantitative traits was confi rmed

THE EFFECT OF CLPS AND RYR1 GENE POLYMORPHISM ON MEAT QUALITY OF ZŁOTNICKA SPOTTED PIGS

The aim of the study was to relate the polymorphisms at CLPS and RYR1 genes to pork quality in case of Złotnicka Spotted breed. The experiment was carried out on 30 purebred Złotnicka Spotted pigs, which were reared in standard conditions and slaughtered at approximately 100 to 110 kg body weight. Genetic polymorphisms were determined with the PCR-RFLP procedure. Polymorphism at the colipase gene (CLPS) was identified with DdeI restriction enzyme and stress sensibility gene (RYR1) with HinPI. In the studied pig sample three genotypes at locus CLPS/DdeI and two genotypes at locus RYR1/HinPI were found. The genotype frequencies at CLPS gene were 0.07 for AA, 0.40 for AB and 0.53 for BB. Colipase gene affected intramuscular fat content in RYR1/CT pigs. Meat from pigs with genotype BB had higher intramuscular fat content (2.50%) compared to AA group (1.59%); P≤0.05. Moreover, significant influence of gene RYR1T on pH1 trait was shown. Values of all analyzed traits were within ranges characterizing good meat quality of the native Polish Złotnicka Spotted pig