Validation and Improvement of High Nature Value Farmland Identification - National Approach in the Walloon Region in Belgium and in the Czech Republic

The concept of High Nature Value (HNV) farmland has been evolving over the last fifteen years in Europe. In the European Union this has been closely linked to the aim of integrating environmental concerns in the Common Agricultural Policy. The idea that nature values, environmental qualities, even cultural heritage are linked to or dependent on farming, also underlies and supports the concept of a multifunctional 'European model of farming which provides benefits beyond food production. The objective of this study is to better identify and characterise HNV farmland at national level (the Walloon Region in Belgium) with a Farm System Approach based on FSS statistical data and specific national surveys, taking into account the whole farm with the total agricultural area and its characteristics. The methodology is built on two different approaches: the natural zones approach and the farm system approach. The natural zones approach uses spatial data to define agricultural zones with a verified high biodiversity interest, based on a reference biodiversity layer for the Walloon Region (WR) called the principal ecological structure (SEP). The farm system approach analyses the farm structure and farming practices on the basis of data available in the WR agricultural database. In the first step relevant variables have been selected to calculate the HNV indicators for the farm system approach at NUTS 5 level: 1) crop diversity and grassland presence, 2) extensive practices, and 3) landscape elements. In the second step the three indicators were combined to calculate the HNV score. In the third step the areas delimited through the farm system approach were overlaid with a map resulting form the natural zones approach. In the last step HNV farmland was analysed with regard to prevailing trends in farming practices and production types with the aim of identification of farming systems which might be associated with HNV farmland occurrence in the WR in Belgium. The analysis of spatial distribution of HNV farmland delimited in the study shows that 91% of the HNV farm system zone identified in the study is located in Ardenne, Fagne and Fammenne. The dominant farming system types are grazing livestock system specialised in milk or meat production, depending on the region. The average livestock density is less than 2.0 LU/ha. Over 60% of UAA is used as permanent grassland. The strength of the Farm System Approach methodology is the possibility of producing a map at the NUTS 5 level with an accurate description of the farm systems. The methodology can be improved by crosschecking the results with regional experts and getting feedback on the thresholds and the weighting of the indicators. The weaknesses of the Farm System Approach methodology are mainly due to the way agricultural practices are modelled. The lack of comprehensive and reliable data on landscape elements and grassland management hampers further validation of HNV farmland identification results. A development of additional relevant surveys is therefore recommended. An attempt at assessing the applicability of the FSA for HNV farmland identification was undertaken for the Czech Republic. Statistical data on farming practices are still incomplete and insufficient to build the methodology similar to the Walloon Region FSA. Conversely, biodiversity and habitat data are abundant and of high quality and currently are the best datasets for HNV farmland identification in the Czech Republic.JRC.H.5-Rural, water and ecosystem resource

Low Input Farming Systems: an Opportunity to Develop Sustainable Agriculture - Proceedings of the JRC Summer University - Ranco, 2-5 July 2007

The concept of sustainability applied to agriculture developed mainly as a result of growing awareness of negative impacts of intensive farming systems on the environment and the quality of life of rural and neighbouring communities. Intensive farming systems are based on genetically uniform crops and livestock breeds, vulnerable to pests and diseases. High yields are obtained through dependency on external inputs (especially fossil energy, fertilizers and pesticides) which can cause decreased air, water, soil and food quality. Intensification and specialisation also bring about landscape changes, resulting in its homogenisation and destruction of traditional landscape elements and, consequently, loss of habitats. Marginal areas, on the other hand, are threatened with cessation of agricultural practices and land abandonment. All these factors also lead, directly or indirectly, to the loss of biodiversity. Lewandowski et al. defined in 1999 sustainable agriculture as ¿the management and utilization of the agricultural ecosystem in a way that maintains its biological diversity, productivity, regeneration capacity, vitality, and ability to function, so that it can fulfill ¿ today and in the future ¿ significant ecological, economic and social functions at the local, national and global levels and does not harm other ecosystems¿. The search for sustainability of agriculture inevitably leads to the exploration of the potential of Low Input Farming Systems (LIFS) to achieve this goal. Within this context, the European Commission Joint Research Centre, Institute for Environment and Sustainability and SOLAGRO organized a Summer University ¿Low Input Farming Systems: an Opportunity to Develop Sustainable Agriculture¿ which took place on 2-5 July 2007 in Ranco (Italy). The programme of the Summer University has been drawn to reflect the diversity of Low Input Farming Systems in Europe and the complexity of factors currently impacting on European agriculture. This report contains the papers which were presented at the meeting as well as final conclusions, summarizing the main points of the discussions which suggested possible lines of future research and policy options which might support LIFS in EuropeJRC.H.5-Rural, water and ecosystem resource

Identification of High Nature Value Farmland in France through Statistical Information and Farm Practices Surveys

The Concept of High Nature Value (HNV) farmland has been evolving over the last fifteen years in Europe. In the European Union this has been closely linked to the aim of integrating environmental concerns in the Common Agricultural Policy. The idea that nature values, environmental qualities, even cultural heritage are linked to or dependent on farming, also underlies and supports the concept of a multifunctional 'European model of farming' which provides benefits other than food. The 'High Nature Value farming' idea thus ties the preservation of biodiversity and wildlife value of the countryside to the need to safeguard the continuation of farming in certain areas with maintenance of specific farming systems associated to the long-term management of these areas. The work presented in the report aims at better identifying and characterising HNV farmland at national level (France) with a farm system approach based on FSS statistical data and specific national surveys, taking into account the whole farm with the total agricultural area and its characteristics. In a first step relevant variables have been selected to calculate the “crop diversity” indicator at NUTS 5. In a second step the crop diversity indicator has been combined overlaid with other data sets from other surveys (“Grassland” Survey and “Forestry” Survey) to build “the landscape elements” and “the extensive crops and grasslands” indicators. In a third step these three indicators have been weighted to calculate a final score and draw maps at municipality scale (NUTS 5).JRC.H.5-Rural, water and ecosystem resource

Developing ecosystem service indicators: experiences and lessons learned from sub-global assessments and other initiatives

People depend upon ecosystems to supply a range of services necessary for their survival and well-being. Ecosystem service indicators are critical for knowing whether or not these essential services are being maintained and used in a sustainable manner, thus enabling policy makers to identify the policies and other interventions needed to better manage them. As a result, ecosystem service indicators are of increasing interest and importance to governmental and inter-governmental processes, including amongst others the Convention on Biological Diversity (CBD) and the Aichi Targets contained within its strategic plan for 2011-2020, as well as the emerging Intergovernmental Platform on Biodiversity and Ecosystem Services (IPBES). Despite this growing demand, assessing ecosystem service status and trends and developing robust indicators is o!en hindered by a lack of information and data, resulting in few available indicators. In response, the United Nations Environment Programme World Conservation Monitoring Centre (UNEP-WCMC), together with a wide range of international partners and supported by the Swedish International Biodiversity Programme (SwedBio)*, undertook a project to take stock of the key lessons that have been learnt in developing and using ecosystem service indicators in a range of assessment contexts. The project examined the methodologies, metrics and data sources employed in delivering ecosystem service indicators, so as to inform future indicator development. This report presents the principal results of this project

Interaction of nicotinic receptors with bupropion: Structural, functional, and pre-clinical perspectives

Besides the antidepressant activity of bupropion (BP), preclinical studies in rodents provide evidence that this compound and its hydroxyl metabolites can attenuate nicotine withdrawal, reversing both the physical and negative affective aspects of nicotine abstinence. Co-interactions of BP with nicotine or other psychostimulants influence decrease anxiety- and cognitive- related processes. BP also attenuates the reinstatement of nicotine-induced conditioned place preference in rats caused by a priming dose of nicotine, morphine, cannabinoids, or ethanol. Therefore, BP can offer an interesting approach to the prevention of relapse in humans. There is emerging evidence that BP inhibits, in the low to intermediate micromolar range, various nicotinic acetylcholine receptors (AChRs) expressed in different neuronal pathways. The BP selectivity for different AChRs follows the sequence: α3- > α4- ~ α1- > α7-containing AChRs. This receptor blockade may contribute to its dual therapeutic activity as either an antidepressant or anti-nicotinic drug. Regarding the structural aspects, two distinct binding sites for [125I]SADU-3-72, a photosensitive derivative of BP, were identified in the Torpedo AChR. A binding site is located within the ion channel, which coincides with the molecular docking results, whereas a second site is found near the extracellular end of α1-M1 when the receptor is in the desensitized state. Interestingly, BP greatly reduces the potentiating action elicited by Zn2+ on different non-α7 AChRs, and vice versa this cation increases the inhibitory strength of BP. This contrasting behavior supports the concept that the binding sites for BP and Zn2+are located at different domains. The understanding of the BP activity, alone or in combination with other drugs (e.g., nicotine), at the molecular and behavioral levels, may improve the knowledge of the underlying mechanisms of action. This knowledge is essential for the development of novel BP (or other antidepressant) derivatives with improved clinical profiles for the treatment of depression and psychostimulant-related addictions

Sequence and Temperature Influence on Kinetics of DNA Strand Displacement at Gold Electrode Surfaces

Understanding complex contributions of surface environment to tethered nucleic acid sensing experiments has proven challenging, yet it is important because it is essential for interpretation and calibration of indispensable methods, such as microarrays. We investigate the effects of DNA sequence and solution temperature gradients on the kinetics of strand displacement at heated gold wire electrodes, and at gold disc electrodes in a heated solution. Addition of a terminal double mismatch (toehold) provides a reduction in strand displacement energy barriers sufficient to probe the secondary mechanisms involved in the hybridization process. In four different DNA capture probe sequences (relevant for the identification of genetically modified maize MON810), all but one revealed a high activation energy up to 200 kJ/mol during hybridization, that we attribute to displacement of protective strands by capture probes. Protective strands contain 4 to 5 mismatches to ease their displacement by the surface-confined probes at the gold electrodes. A low activation energy (30 kJ/mol) was observed for the sequence whose protective strand contained a toehold and one central mismatch, its kinetic curves displayed significantly different shapes, and we observed a reduced maximum signal intensity as compared to other sequences. These findings point to potential sequence-related contributions to oligonucleotide diffusion influencing kinetics. Additionally, for all sequences studied with heated wire electrodes, we observed a 23 K lower optimal hybridization temperature in comparison with disc electrodes in heated solution, and greatly reduced voltammetric signals after taking into account electrode surface area. We propose that thermodiffusion due to temperature gradients may influence both hybridization and strand displacement kinetics at heated microelectrodes, an explanation supported by computational fluid dynamics. DNA assays with surface-confined capture probes and temperature gradients should not neglect potential influences of thermodiffusion as well as sequence-related effects. Furthermore, studies attempting to characterize surface-tethered environments should consider thermodiffusion if temperature gradients are involved