Measuring Electric Charge and Molecular Coverage on Electrode Surface from Transient Induced Molecular Electronic Signal (TIMES).

Charge density and molecular coverage on the surface of electrode play major roles in the science and technology of surface chemistry and biochemical sensing. However, there has been no easy and direct method to characterize these quantities. By extending the method of Transient Induced Molecular Electronic Signal (TIMES) which we have used to measure molecular interactions, we are able to quantify the amount of charge in the double layers at the solution/electrode interface for different buffer strengths, buffer types, and pH values. Most uniquely, such capabilities can be applied to study surface coverage of immobilized molecules. As an example, we have measured the surface coverage for thiol-modified single-strand deoxyribonucleic acid (ssDNA) as anchored probe and 6-Mercapto-1-hexanol (MCH) as blocking agent on the platinum surface. Through these experiments, we demonstrate that TIMES offers a simple and accurate method to quantify surface charge and coverage of molecules on a metal surface, as an enabling tool for studies of surface properties and surface functionalization for biochemical sensing and reactions

The Effects of Force on the Structure Deformation of Wing for Flapping-wing

This paper investigated the effects of aerodynamic force and inertial force on the structure deformation of wing. The aerodynamic force was tested from the wind tunnel experiment. The study indicated the quantity of aerodynamic force and inertial force is equal. The maximum deformation was produced by aerodynamic force or resultant force when wing is located on horizontal situation. The study of wing structure deformation provide guide for design and optimization of wing for flapping-wing.Keywords: Flapping-wing; aerodynamic force; inertial force; structure deformatio

A convenient tandem one-pot synthesis of donor-acceptor-type triphenylene 2,3-dicarboxylic esters from diarylacetylene

A tandem one-pot method for the direct synthesis of polysubstituted triphenylene 2,3-dicarboxylic esters with different substitution patterns was developed by enyne metathesis of diarylacetylene, followed by Diels–Alder, aromatization and a cyclization cascade

Detection of B. anthracis Spores and Vegetative Cells with the Same Monoclonal Antibodies

Bacillus anthracis, the causative agent of anthrax disease, could be used as a biothreat reagent. It is vital to develop a rapid, convenient method to detect B. anthracis. In the current study, three high affinity and specificity monoclonal antibodies (mAbs, designated 8G3, 10C6 and 12F6) have been obtained using fully washed B. anthracis spores as an immunogen. These mAbs, confirmed to direct against EA1 protein, can recognize the surface of B. anthracis spores and intact vegetative cells with high affinity and species-specificity. EA1 has been well known as a major S-layer component of B. anthracis vegetative cells, and it also persistently exists in the spore preparations and bind tightly to the spore surfaces even after rigorous washing. Therefore, these mAbs can be used to build a new and rapid immunoassay for detection of both life forms of B. anthracis, either vegetative cells or spores

Effect of Water Distribution during Pre-drying on the Microstructure and Texture Properties of Peach Crisps Produced by Hot Air-Vacuum Freeze Drying

In this study, experiments were conducted to investigate the effect of moisture distribution during pre-drying on the microstructure and textural quality of hot air-vacuum freeze dried peach slices. The moisture distribution during the hot air pre-drying process at different temperatures (40, 60 and 80 ℃) and the product temperature during heating were monitored. Three levels of dry-basis moisture content (7, 6 and 5 g/g) were selected as moisture conversion points for each temperature. The color, shrinkage rate, microstructure, pore distribution, textural properties and hygroscopicity of peach crisps were measured. The results showed that drying temperature had a great impact on the moisture distribution during the pre-drying process, but the overall trends of moisture mobility were consistent among the different drying temperatures. The lower the moisture content of the pre-dried sample, the closer the color of the final dried sample to that of the fresh sample. The color of the sample dried at 40 ℃ with a moisture conversion point of 5 g/g was the closest to that of the fresh sample. Drying time had a greater effect on the shrinkage rate than temperature. It took longer to dry peach slices to the same moisture conversion point at 40 ℃ than 60 and 80 ℃. The sample shrank distinctly during both pre-drying and combined drying. There was a significant difference in the pore structure between the freeze-dried and combined dried samples. The sample with a moisture conversion point of 5 g/g had the most heterogeneous pore structure. The average hardness value of the hot air-vacuum freeze dried sample increased by 52.11% compared with that of the freeze-dried sample. The lower the moisture content of the pre-dried sample, the higher the hardness value of the hot air-vacuum freeze dried sample. This study showed that hot air pre-drying can effectively control the crunchiness and hardness of peach crisps. The decrease in the hygroscopicity of the hot air-vacuum freeze dried sample compared with the vacuum freeze dried one may be related to the structure changes during the pre-drying process. In summary, hot air-vacuum freeze drying is conducive to improving the texture quality and storage stability of peach crisps than vacuum freeze drying

Activation of MET signaling by HDAC6 offers a rationale for a novel ricolinostat and crizotinib combinatorial therapeutic strategy in diffuse large B‐cell lymphoma

Some histone deacetylases (HDACs) promote tumor cell growth and pan‐ or selective HDAC inhibitors are active in some cancers; however, the pivotal HDAC enzyme and its functions in human diffuse large B‐cell lymphoma (DLBCL) remain largely unknown. Using NanoString nCounter assays, we profiled HDAC mRNA expression and identified HDAC6 as an upregulated HDAC family member in DLBCL tissue samples. We then found that HDAC6 plays an oncogenic role in DLBCL, as evidenced by its promotion of cell proliferation in vitro and tumor xenograft growth in vivo. Mechanistically, the interaction between HDAC6 and HR23B downregulated HR23B expression, thereby reducing the levels of casitas B‐lineage lymphoma (c‐Cbl), an E3 ubiquitin ligase for hepatocyte growth factor receptor (MET), which resulted in the inhibition of MET ubiquitination‐dependent degradation. In addition, enhanced HDAC6 expression and decreased HR23B expression were correlated with poor overall survival rates among patients with DLBCL. Taken together, these results establish an HDAC6–HR23B–MET axis and indicate that HDAC6 is a potent promoter of lymphomagenesis in DLBCL. Thus, a therapeutic strategy based on HDAC6 inhibitors in combination with MET inhibitors is promising. Copyright © 2018 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/146400/1/path5108_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/146400/2/path5108.pd

The therapeutic potential of GABA in neuron-glia interactions of cancer-induced bone pain

Abstract: The development of effective therapeutics for cancer-induced bone pain (CIBP) remains a tremendous challenge owing to its unclear mechanisms. Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the central nervous system. Emerging studies have shown that disinhibition in the spinal cord dorsal horn may account for the development of chronic pain. However, the role of GABA in the development of CIBP remains elusive. In addition, accumulating evidence has shown that neuroglial cells in the peripheral nervous system, especially astrocytes and microglial cells, play an important role in the maintenance of CIBP. In this study, we investigated the expression of GABA and Gamma-aminobutyric acid transporter-1 (GAT-1), a transporter of GABA. Our results demonstrate that GABA was decreased in CIBP rats as expected. However, the expression of glutamic acid decarboxylase (GAD) 65 was up-regulated on day 21 after surgery, while the expression of glutamic acid decarboxylase (GAD) 67 remained unchanged after surgery. We also found that the expression of GAT-1 was up-regulated mainly in the astrocytes of the spinal cord. Moreover, we evaluated the analgesic effect of exogenous GABA and the GAT-1 inhibitor. Intrathecal administration of exogenous GABA and NO-711(a GAT-1 selective inhibitor) significantly reversed CIBP-induced mechanical allodynia in a dose-dependent manner. These results firstly show that neuron-glia interactions, especially on the GABAnergic pathway, contribute to the development of CIBP. In conclusion, exogenous GABA and GAT-1 inhibitor might be alternative therapeutic strategies for the treatment of CIBP. Keywords: Cancer-induced bone pain; Gamma-Aminobutyric acid; Glutamic acid decarboxylases; GABA transporters; NO-711; Astrocyt

Far-field transient absorption nanoscopy with sub-50 nm optical super-resolution

Nanoscopic imaging or characterizing is the mainstay of the development of advanced materials. Despite great progress in electronic and atomic force microscopies, label-free and far-field characterization of materials with deep sub- wavelength spatial resolution has long been highly desired. Herein, we demonstrate far-field super-resolution transient absorption (TA) imaging of two-dimensional material with a spatial resolution of sub-50 nm. By introducing a donut- shaped blue saturation laser, we effectively suppress the TA transition driven by near-infrared (NIR) pump–probe photons, and push the NIR-TA microscopy to sub-diffraction-limited resolution. Specifically, we demonstrate that our method can image the individual nano-grains in graphene with lateral resolution down to 36 nm. Further, we perform super-resolution TA imaging of nano-wrinkles in monolayer graphene, and the measured results are very consistent with the characterization by an atomic force microscope. This direct far-field optical nanoscopy holds great promise to achieve sub-20 nm spatial resolution and a few tens of femtoseconds temporal resolution upon further improvement and represents a paradigm shift in a broad range of hard and soft nanomaterial characterization