Evaluation of the Amphibian Fauna of the Semi-arid and Arid Grassland Habitats in and around the Mayureshwar, Rehekuri, and Karmala Wildlife Sanctuaries in Maharashtra, India

Few studies document amphibian species composition from arid and semiarid environments. Scatteredbodies of water in the semi-arid and arid landscape around Pune, Maharashtra, India, could be viable amphibian habitats; however; information on amphibians using these bodies of water is not available. In a preliminary study, we assessed the amphibian diversity and population status in three wildlife sanctuaries and surrounding areas near Pune. We also compared the use of artificial and natural ponds by amphibians and surveyed the amphibians for the presence of the chytrid fungus

Different stimulus types and long-term plasticity in mouse primary visual cortex

Evidence points to the existence of long-term, experience-dependent plasticity in primary visual cortex (V1). This is surprising given the early location of V1 in the visual processing hierarchy, but is consistent with known anatomical and functional properties of V1 circuitry. Research has uncovered, with training, the emergence of stimulus- specific potentiation and complex “predictive” activity in V1. The Berry lab has previously explored encoding of novel and familiar visual stimuli in layer 2/3 excitatory neurons, along with the short-term stimulus memory capacity of these cells. Prior experiments used sequences of custom stimuli (Gabor patches) and two-photon calcium imaging, which enables in vivo recording from cortical neurons at single-cell resolution. I investigated whether training with sequences—made from different stimuli types—affects how layer 2/3 cells encode sequences. I performed two-day experiments using naturalistic movies, naturalistic scenes, and static gratings. I focused on "sustained" cells, or neurons responding periodically to part of a sequence while it is presented. Sustained responses may encode both spatial and temporal features of a sequence, but such responses have not been compared across different stimuli. For each experiment, analysis questions included how sustained responses changed in magnitude and temporal dynamics within and across days. Results indicate substantial overall plasticity, between days, for all stimulus types—including amplitude enhancement that can be stronger than Gabors-induced learning effects. During experiments with movies, population activity in response to "blank" frame substitutions—when no stimulus is shown—increasingly recapitulated the activity during uninterrupted movies. Such “filling in” had greater fidelity when blank frames are presented in the same fixed position (instead of random positions) within a movie. The findings are consistent with the basic expectations of a predictive coding model for V1. While thesis results have substantial limitations, they also suggest avenues for new experimentation

A molecularly integrated grade for meningioma.

BackgroundMeningiomas are the most common primary intracranial tumor in adults. Clinical care is currently guided by the World Health Organization (WHO) grade assigned to meningiomas, a 3-tiered grading system based on histopathology features, as well as extent of surgical resection. Clinical behavior, however, often fails to conform to the WHO grade. Additional prognostic information is needed to optimize patient management.MethodsWe evaluated whether chromosomal copy-number data improved prediction of time-to-recurrence for patients with meningioma who were treated with surgery, relative to the WHO schema. The models were developed using Cox proportional hazards, random survival forest, and gradient boosting in a discovery cohort of 527 meningioma patients and validated in 2 independent cohorts of 172 meningioma patients characterized by orthogonal genomic platforms.ResultsWe developed a 3-tiered grading scheme (Integrated Grades 1-3), which incorporated mitotic count and loss of chromosome 1p, 3p, 4, 6, 10, 14q, 18, 19, or CDKN2A. 32% of meningiomas reclassified to either a lower-risk or higher-risk Integrated Grade compared to their assigned WHO grade. The Integrated Grade more accurately identified meningioma patients at risk for recurrence, relative to the WHO grade, as determined by time-dependent area under the curve, average precision, and the Brier score.ConclusionWe propose a molecularly integrated grading scheme for meningiomas that significantly improves upon the current WHO grading system in prediction of progression-free survival. This framework can be broadly adopted by clinicians with relative ease using widely available genomic technologies and presents an advance in the care of meningioma patients

Targeted gene expression profiling predicts meningioma outcomes and radiotherapy responses

Surgery is the mainstay of treatment for meningioma, the most common primary intracranial tumor, but improvements in meningioma risk stratification are needed and indications for postoperative radiotherapy are controversial. Here we develop a targeted gene expression biomarker that predicts meningioma outcomes and radiotherapy responses. Using a discovery cohort of 173 meningiomas, we developed a 34-gene expression risk score and performed clinical and analytical validation of this biomarker on independent meningiomas from 12 institutions across 3 continents (N = 1,856), including 103 meningiomas from a prospective clinical trial. The gene expression biomarker improved discrimination of outcomes compared with all other systems tested (N = 9) in the clinical validation cohort for local recurrence (5-year area under the curve (AUC) 0.81) and overall survival (5-year AUC 0.80). The increase in AUC compared with the standard of care, World Health Organization 2021 grade, was 0.11 for local recurrence (95% confidence interval 0.07 to 0.17, P &lt; 0.001). The gene expression biomarker identified meningiomas benefiting from postoperative radiotherapy (hazard ratio 0.54, 95% confidence interval 0.37 to 0.78, P = 0.0001) and suggested postoperative management could be refined for 29.8% of patients. In sum, our results identify a targeted gene expression biomarker that improves discrimination of meningioma outcomes, including prediction of postoperative radiotherapy responses.</p

Targeted gene expression profiling predicts meningioma outcomes and radiotherapy responses

Surgery is the mainstay of treatment for meningioma, the most common primary intracranial tumor, but improvements in meningioma risk stratification are needed and indications for postoperative radiotherapy are controversial. Here we develop a targeted gene expression biomarker that predicts meningioma outcomes and radiotherapy responses. Using a discovery cohort of 173 meningiomas, we developed a 34-gene expression risk score and performed clinical and analytical validation of this biomarker on independent meningiomas from 12 institutions across 3 continents (N = 1,856), including 103 meningiomas from a prospective clinical trial. The gene expression biomarker improved discrimination of outcomes compared with all other systems tested (N = 9) in the clinical validation cohort for local recurrence (5-year area under the curve (AUC) 0.81) and overall survival (5-year AUC 0.80). The increase in AUC compared with the standard of care, World Health Organization 2021 grade, was 0.11 for local recurrence (95% confidence interval 0.07 to 0.17, P &lt; 0.001). The gene expression biomarker identified meningiomas benefiting from postoperative radiotherapy (hazard ratio 0.54, 95% confidence interval 0.37 to 0.78, P = 0.0001) and suggested postoperative management could be refined for 29.8% of patients. In sum, our results identify a targeted gene expression biomarker that improves discrimination of meningioma outcomes, including prediction of postoperative radiotherapy responses.</p

Targeted gene expression profiling predicts meningioma outcomes and radiotherapy responses

Surgery is the mainstay of treatment for meningioma, the most common primary intracranial tumor, but improvements in meningioma risk stratification are needed and indications for postoperative radiotherapy are controversial. Here we develop a targeted gene expression biomarker that predicts meningioma outcomes and radiotherapy responses. Using a discovery cohort of 173 meningiomas, we developed a 34-gene expression risk score and performed clinical and analytical validation of this biomarker on independent meningiomas from 12 institutions across 3 continents (N = 1,856), including 103 meningiomas from a prospective clinical trial. The gene expression biomarker improved discrimination of outcomes compared with all other systems tested (N = 9) in the clinical validation cohort for local recurrence (5-year area under the curve (AUC) 0.81) and overall survival (5-year AUC 0.80). The increase in AUC compared with the standard of care, World Health Organization 2021 grade, was 0.11 for local recurrence (95% confidence interval 0.07 to 0.17, P &lt; 0.001). The gene expression biomarker identified meningiomas benefiting from postoperative radiotherapy (hazard ratio 0.54, 95% confidence interval 0.37 to 0.78, P = 0.0001) and suggested postoperative management could be refined for 29.8% of patients. In sum, our results identify a targeted gene expression biomarker that improves discrimination of meningioma outcomes, including prediction of postoperative radiotherapy responses