Optimization of rTDMH as a Reagent Toward Improving the Sensitivity of the RT-PCR Based Diagnosis for Mycobacterium Tuberculosis

Current diagnostic tools being used for tuberculosis lack the speed and sensitivity necessary to successfully combat the current tuberculosis epidemic. Real-time Polymerase Chain Reaction, RT-PCR, can provide the rapid and specific diagnosis that is currently in demand in the global community. Its disadvantage is that due to the waxy and robust nature of the M. tuberculosis membrane, not enough genomic DNA is present to provide for amplification in a RT-PCR. It was previously found in our laboratory that hydrolysis of one of abundant glycolipid of mycobacterial envelope, Trehalose, 6,6’-dimycolate, by a recombinant TDM-specific hydrolase caused rapid lysis of cell (Yong et.al. manuscript submitted). In this study, we tested if rapid lysis by TDM-specific hydrolase (rTDMH) can be exploited in conjunction with the RT-PCR to develop a sensitive diagnosis of tuberculosis. Results demonstrated that by incubation of both attenuated M. tuberculosis, and virulent M. tuberculosis with rTDMH for lysis and subsequent usage of this lysate in a RT-PCR assay, yields sensitive amplification of mycobacterial DNA. rTDMH-mediated lsyis could facilitate amplification of even 10 bacilli, the rTDMH treated cells show amplification while lack of treatment failed to detect these bacilli These results were consistent in in-vitro liquid culture and in complex sputum samples spiked with the mycobacteria, showing that incubation with rTDMH can improve the sensitivity of the RT-PCR. Statement of Public Health relevance: Using rTDMH with RT-PCR as an improved diagnostic tool for tuberculosis due to the rapid, accurate and sensitive nature of the assay could provide the global community with a much better method of diagnosing a disease that has plagued the world for thousands of years. Tuberculosis infects 9 million people and kills 3 million people every year and presently one-third of the world’s population is infected with it. A better diagnostic tool could result in reducing the spread of disease; reducing the mortality associated with disease, especially in HIV infected individuals; and on a broader scale, could reduce the economic burden associated with the diseas

Iqbal and Goethe : a note

The recourse to Goethe plays an important role in the work of Mohammad Iqbal (1873-1938), one of the few important writers from the Indian subcontinent who knew German literature. Iqbal situates his own writing in the context of western colonial expansion and the corresponding world-historical loss of power of Islam in the East. The recourse to Goethe becomes an import reference point in his work. It enables him to stylise himself as a Messenger of the East in reply to Goethe as a representative of the West. By establishing a comparative cultural constellation with his German predecessor Iqbal affirms a cultural position consisting of a mode of historical complaint and cultural revival

Willy Haas (1891-1973) : "homme de lettre"

There are many aspects of Haas' life and experiences in India which deserve greater attention. I would like to refer briefly only to his attempts as a litterateur to come to terms with 'India' as presented in his autobiographical recollection and to some comparative cultural reflections in his essays. Like all reconstructions his autobiographical recollection of India is also a construct in which the site of India as a place of exile is justified by an achieved awareness between conscious individual choice and inevitability. An individual acts out a personal history, the prefiguration of which he only becomes aware of in the form of a subsequent epiphanic realization. Given Haas' literary background, it is not surprising that this is articulated through a literary association

Jet Physics at the Tevatron

Jets have been used to verify the theory of quantum chromodynamics (QCD), measure the structure of the proton and to search for the physics beyond the Standard Model. In this article, we review the current status of jet physics at the Tevatron, a sqrt(s) = 1.96 TeV p-pbar collider at the Fermi National Accelerator Laboratory. We report on recent measurements of the inclusive jet production cross section and the results of searches for physics beyond the Standard Model using jets. Dijet production measurements are also reported.Comment: 29 pages, 12 figures, submitted to Ann.Rev.Nucl.Part.Sc

Pilot-aided carrier synchronization using an approximate DCT-based phase noise model

This contribution deals with phase noise estimation from pilot symbols. The phase noise process is approximated by an expansion of DCT basis functions containing only a few terms. We propose an algorithm that estimates the DCT coefficients without requiring detailed knowledge about the phase noise statistics. We demonstrate that the resulting (linearized) mean-square estimation error consists of two contributions: a contribution from the additive noise, that equals the Cramer-Rao lower bound, and a noise-independent contribution that results from the phase noise modeling error. Performance can be optimized by a proper selection of the symbol block length and of the number of DCT coefficients to be estimated. For large block sizes, considerable performance improvement is found as compared to the case where only the time-average of the carrier phase is estimated

Feedforward data-aided phase noise estimation from a DCT basis expansion

This contribution deals with phase noise estimation from pilot symbols. The phase noise process is approximated by an expansion of discrete cosine transform (DCT) basis functions containing only a few terms. We propose a feedforward algorithm that estimates the DCT coefficients without requiring detailed knowledge about the phase noise statistics. We demonstrate that the resulting (linearized) mean-square phase estimation error consists of two contributions: a contribution from the additive noise, that equals the Cramer-Rao lower bound, and a noise independent contribution, that results front the phase noise modeling error. We investigate the effect of the symbol sequence length, the pilot symbol positions, the number of pilot symbols, and the number of estimated DCT coefficients it the estimation accuracy and on the corresponding bit error rate (PER). We propose a pilot symbol configuration allowing to estimate any number of DCT coefficients not exceeding the number of pilot Symbols, providing a considerable Performance improvement as compared to other pilot symbol configurations. For large block sizes, the DCT-based estimation algorithm substantially outperforms algorithms that estimate only the time-average or the linear trend of the carrier phase. Copyright (C) 2009 J. Bhatti and M. Moeneclaey

Feedforward pilot-aided carrier synchronization using a DCT basis expansion

This contribution deals with phase noise estimation from pilot symbols. The phase noise process is approximated by an expansion of Discrete Cosine-Transform (DCT) basis functions containing only a few terms. We propose a feedforward algorithm that estimates the DCT coefficients without requiring detailed knowledge about the phase noise statistics. We demonstrate that the resulting (linearized) mean-square phase estimation error consists of two contributions: a contribution from the additive noise, that equals the Cramer-Rao lower bound, and a noise-independent contribution that results from the phase noise modeling error. We investigate the effect of the symbol sequence length and the number of estimated DCT coefficients on the estimation accuracy and on the corresponding bit error rate (BER). We propose a pilot symbol configuration allowing to estimate any number of DCT coefficients not exceeding the number of pilot symbols. For large block sizes, the DCT-based estimation algorithm substantially outperforms algorithms that estimate only the time-average or the linear trend of the carrier phase

Do Expectations Play Any Role in Determining Pak Rupee Exchange Rates?

This paper presents some evidence on the role of expectations in the determination of Pak rupee exchange rates vis-à-vis the dollar, pound, and yen over the period 1982:1– 1993:7. Results of cointegration and coefficient restriction tests in two out of three cases are supportive of the view of exchange rate determination in postulating that in efficient markets in which uncertainty and expectations about the future are dominant, the equilibrium nominal exchange rate is determined not only by current relative prices but also by the expected real exchange rate. These results are supportive of ex ante purchasing power parity, implying that the real exchange rate follows a random walk. These results also suggest that the anticipated inflation rate is higher in Pakistan than in other countries, which tends to encourage the domestic residents to convert their current balances into foreign currency, so that the terms of trade deteriorate and offset much of gains of the continuous devaluation of Pak rupee by undermining external competitiveness.

Growth and Poverty in Pakistan: Implications for Governance

According to the Economic Surveys, Pakistan’s real GDP has grown at an average annual rate of 6.8 percent in the 1960s, 4.8 percent in the 1970s, 6.5 percent in the 1980s and 4.7 percent in the 1990s. However, that did not seem to have mitigated poverty as parallel to this growth the number of poor also kept swelling. Although different estimates put number of poor in Pakistan around 50 million, the actual could be more [Ahmad (2001)]. The average growth rates in the first halfcentury of Pakistan have been around 2 percent [Hasan (1997)]. It is pertinent to state that this discussion paper is not an attempt to challenge the figures either of the growth rates or the numbers of the poor in Pakistan. This is rather an attempt to understand the correlation of governance with growth on one hand and poverty on the other. It offers conceptual analysis of the concepts and their respective interpretation, explanation, application and ensuing misunderstandings. This paper has also attempted to challenge certain (usual) assumptions and perceptions regarding the role and relationship of growth and governance in reducing poverty in Pakistan. One has pointed out that most of the studies on the subject focus on symptoms and not the causes of poverty. This leads to on one hand growth of poverty, as poverty does not seem to halt despite certain evidence of relatively high growth particularly in 1960s. On the other hand we witness poverty of growth as whatever growth we have had it has hitherto failed either translating into corresponding mitigation of poverty or equitable collective prosperity. This is because there have not been efforts at governance level to ensure equity of impact of growth through adequate distribution mechanisms, sufficient social and human investments leading to education and skill development of women and men, who in turn could benefit from opportunities arising by way of process of economic growth.