The Impact of Climate Change on Biodiversity in Nepal: Current Knowledge, Lacunae, and Opportunities

Nepal has an extreme altitudinal range from 60–8850m with heterogeneous topography and distinct climatic zones. The country is considered a biodiversity hotspot, with nearly a quarter of the land area located in protected areas. Nepal and the surrounding Himalayan region are particularly vulnerable to climate change because of their abrupt ecological and climatic transitions. Tens of millions of people rely on the region’s ecosystem services, and observed and modeled warming trends predict increased climate extremes in the Himalayas. To study the ecological impacts of climate change in Nepal and inform adaptation planning, we review the literature on past, present, and predicted future climatic changes and their impacts on ecological diversity in Nepal. We found few studies focusing on organisms, while research on species and communities was more common. Most studies document or predict species range shifts and changes in community composition. Results of these few investigations highlight major lacunae in research regarding the effects of changing climate on species comprising the Himalayan biota. Further empirical work is needed at all levels of biological organization to build on information regarding direct ecological impacts of climatic changes in the region. Countries face an ever-increasing threat of climate change, and Nepal has strong physiographic, elevational, and climatic gradients that could provide a useful model for studying the effects of climate change on a mountainous, and highly biodiverse, area

Network Structure of Vertebrate Scavenger Assemblages at the Global Scale: Drivers and Ecosystem Functioning Implications

The organization of ecological assemblages has important implications for ecosystem functioning, but little is known about how scavenger communities organize at the global scale. Here, we test four hypotheses on the factors affecting the network structure of terrestrial vertebrate scavenger assemblages and its implications on ecosystem functioning. We expect scavenger assemblages to be more nested (i.e. structured): 1) in species‐rich and productive regions, as nestedness has been linked to high competition for carrion resources, and 2) regions with low human impact, because the most efficient carrion consumers that promote nestedness are large vertebrate scavengers, which are especially sensitive to human persecution. 3) We also expect climatic conditions to affect assemblage structure, because some scavenger assemblages have been shown to be more nested in colder months. Finally, 4) we expect more organized assemblages to be more efficient in the consumption of the resource. We first analyzed the relationship between the nestedness of the scavenger assemblages and climatic variables (i.e. temperature, precipitation, temperature variability and precipitation variability), ecosystem productivity and biomass (i.e. NDVI) and degree of human impact (i.e. human footprint) using 53 study sites in 22 countries across five continents. Then, we related structure (i.e. nestedness) with its function (i.e. carrion consumption rate). We found a more nested structure for scavenger assemblages in regions with higher NDVI values and lower human footprint. Moreover, more organized assemblages were more efficient in the consumption of carrion. However, our results did not support the prediction that the structure of the scavenger assemblages is directly related to climate. Our findings suggest that the nested structure of vertebrate scavenger assemblages affects its functionality and is driven by anthropogenic disturbance and ecosystem productivity worldwide. Disarray of scavenger assemblage structure by anthropogenic disturbance may lead to decreases in functionality of the terrestrial ecosystems via loss of key species and trophic facilitation processes

Scavenging in the Anthropocene: Human impact drives vertebrate scavenger species richness at a global scale

Understanding the distribution of biodiversity across the Earth is one of the most challenging questions in biology. Much research has been directed at explaining the species latitudinal pattern showing that communities are richer in tropical areas; however, despite decades of research, a general consensus has not yet emerged. In addition, global biodiversity patterns are being rapidly altered by human activities. Here, we aim to describe large‐scale patterns of species richness and diversity in terrestrial vertebrate scavenger (carrion‐consuming) assemblages, which provide key ecosystem functions and services. We used a worldwide dataset comprising 43 sites, where vertebrate scavenger assemblages were identified using 2,485 carcasses monitored between 1991 and 2018. First, we evaluated how scavenger richness (number of species) and diversity (Shannon diversity index) varied among seasons (cold vs. warm, wet vs. dry). Then, we studied the potential effects of human impact and a set of macroecological variables related to climatic conditions on the scavenger assemblages. Vertebrate scavenger richness ranged from species‐poor to species rich assemblages (4–30 species). Both scavenger richness and diversity also showed some seasonal variation. However, in general, climatic variables did not drive latitudinal patterns, as scavenger richness and diversity were not affected by temperature or rainfall. Rainfall seasonality slightly increased the number of species in the community, but its effect was weak. Instead, the human impact index included in our study was the main predictor of scavenger richness. Scavenger assemblages in highly human‐impacted areas sustained the smallest number of scavenger species, suggesting human activity may be overriding other macroecological processes in shaping scavenger communities. Our results highlight the effect of human impact at a global scale. As speciesrich assemblages tend to be more functional, we warn about possible reductions in ecosystem functions and the services provided by scavengers in human‐dominated landscapes in the Anthropocene

Vertebrate Scavenger Diversity and Ecosystem Services Along An Elevational Gradient in Central Nepal

A growing number of studies recognize the ecological significance of vertebrate scavengers, and several species belonging to this diverse, functional guild are of high conservation importance around the globe. Studies on taxonomic and functional components of biodiversity often use elevation gradients to comprehensively examine patterns and drivers across multiple spatial scales. Yet, there are relatively few elevational studies on large vertebrates or multi-taxa guilds, and the related variation of their ecosystem services. In particular, scavenger research has largely focused on local-scale studies or regional/global comparisons of local-scale investigations. Moreover, these studies primarily consider taxonomic community characteristics and the patterns of functional diversity remain understudied for vertebrate scavenger communities. In this dissertation, I examine the taxonomic and functional characteristics of vertebrate scavenger assemblages along an elevation and biome gradient in the Chitwan-Annapurna landscape of Central Nepal. Specifically, I present an integration of ecological and socioecological approaches, across four chapters, to develop our understanding of how distribution, structure, function and human perception vary in relation to scavengers across a species-rich, heterogeneous landscape. By monitoring 43 vertebrate scavenger assemblages with remote, motion-triggered cameras, I collected data on community characteristics across three main elevation bands (i.e. lowland, midland, highland) of the gradient. Then, I compiled relevant functional trait characteristics for all recorded species to calculate a range of functional diversity measures that were used across all four chapters. In the first section of the dissertation, I characterize the taxonomic and functional patterns of diversity (Chapter 1) and community structure (Chapter 2) across the gradient. I test a selection of leading hypotheses related to biodiversity distribution, and scavenger diversity in specific, to evaluate the effects of elevation, and other biotic or abiotic factors. For both taxonomic and functional components, I found that richness is jointly driven by elevation and human impact, whereas other environmental factors (i.e. canopy cover, annual temperature range) and human impact shape community composition at local assemblages. While elevation played an overarching role in the distribution of richness within each elevation band as a function of human impact, compositional differences between local assemblages were largely a consequence of environmental filtering, characterized by high taxonomic species turnover and functional redundancy. The latter portion of this dissertation, I focus on the taxonomic and functional characteristics that influence scavengers’ capacity to provide ecosystem functions, and the human-dimension in regard to the perception of these roles across the landscape. I test hypotheses related to the relative contribution of taxonomic and functional trait-based indices on the efficiency of key ecological processes provided by scavengers. I found that carcass detection was influenced by a combination of overall biomass and functional richness, whereas biomass and a greater proportion of carnivores drove carcass consumption rate. Also, I find preliminary evidence of the diversity-stability relationship in a vertebrate scavenging guild, related to the ecosystem function of carcass consumption at assemblages with greater overall biomass and functional richness. Finally, I consider the social perceptions of an important stakeholder in this landscape (i.e. livestock farmers) on the ecosystem service provisioning and functional importance of species in this diverse guild, and assess the influence of sociodemographic traits that may drive these attitudes. I find that farmers only perceive avian scavengers as beneficial ecosystem service providers. However, there was species-specific variation regarding their perception of functional importance. Accordingly, the two responses were coupled for obligate scavengers and decoupled for facultative scavengers that suggests disconnect between the appreciation of ecosystem service and knowledge of ecosystem function for several species (other than vultures) in this guild. Relatedly, I find that affluence-related traits drove positive perceptions of ecosystem service provisioning and local ecological knowledge was related to increased valuation of functional importance. Overall, I highlight the potential role of formal education in shaping positive attitudes towards vertebrate scavengers in this landscape. Correspondingly, I emphasize the need to address latent human-wildlife conflicts that might restrict any perceived value of ecosystem services for facultative scavengers, in spite of environmental education and other forms of local ecological knowledge. The collective findings in this dissertation provides a deeper understanding of the patterns and mechanisms of diversity and ecosystem services for a unique vertebrate scavenger guild, which occurs in a region and at a landscape-scale that is understudied in existing literature. Through this work I build on our scientific knowledge of scavenger ecology and biodiversity-elevation research for vertebrate guilds. I present novel findings of distribution patterns and underlying processes that shape the diversity and ecosystem function of a critical vertebrate guild in a biodiversity hotspot, and explore the multifaceted socioecological relationship between scavengers and humans. Ultimately, these findings may also help inform the identification of key conservation areas, maintain key ecosystem processes, and facilitate inclusive conservation initiatives within the landscape

Dynamics of Cardiovascular Muscle Using a Non-Linear Symmetric Oscillator

In this paper, a complete non-linear symmetric oscillator model using the Hamiltonian approach has been developed and used to describe the cardiovascular conduction process’s dynamics, as the signal generated from the cardiovascular muscle is non-deterministic and random. Electrocardiogram (ECG) signal is a significant factor in the cardiovascular system as most of the medical diagnoses can be well understood by observing the ECG signal’s amplitude. A non-linear cardiovascular muscle model has been proposed in this study, where a modified vanderPol symmetric oscillator-based equation is used. Gone are the days whena non-linear system had been designed using the describing function technique. It is better to design a non-linear model using the Hamiltonian dynamical equation for its high accuracy and flexibility. Varying a non-linear spring constant using this type of approach is more comfortable than the traditional describing function technique. Not only that but different initial conditions can also be taken for experimental purposes. It never affects the overall modeling. The Hamiltonian approach provides the energy of an asymmetric oscillatory system of that cardiovascular conduction system. A non-linear symmetric oscillator was initially depicted by the non-linear mass-spring (two degrees of freedom) model. The motion of an uncertain non-linear cardiovascular system has been solved considering second-order approximation, which also demonstrates the possibility of introducing spatial dimensions. Finally, the model’s natural frequency expression has also been simulated and is composed of the previously published result

Dynamics of Cardiovascular Muscle Using a Non-Linear Symmetric Oscillator

In this paper, a complete non-linear symmetric oscillator model using the Hamiltonian approach has been developed and used to describe the cardiovascular conduction process’s dynamics, as the signal generated from the cardiovascular muscle is non-deterministic and random. Electrocardiogram (ECG) signal is a significant factor in the cardiovascular system as most of the medical diagnoses can be well understood by observing the ECG signal’s amplitude. A non-linear cardiovascular muscle model has been proposed in this study, where a modified vanderPol symmetric oscillator-based equation is used. Gone are the days whena non-linear system had been designed using the describing function technique. It is better to design a non-linear model using the Hamiltonian dynamical equation for its high accuracy and flexibility. Varying a non-linear spring constant using this type of approach is more comfortable than the traditional describing function technique. Not only that but different initial conditions can also be taken for experimental purposes. It never affects the overall modeling. The Hamiltonian approach provides the energy of an asymmetric oscillatory system of that cardiovascular conduction system. A non-linear symmetric oscillator was initially depicted by the non-linear mass-spring (two degrees of freedom) model. The motion of an uncertain non-linear cardiovascular system has been solved considering second-order approximation, which also demonstrates the possibility of introducing spatial dimensions. Finally, the model’s natural frequency expression has also been simulated and is composed of the previously published result

The Impact of Climate Change on Biodiversity in Nepal: Current Knowledge, Lacunae, and Opportunities

Nepal has an extreme altitudinal range from 60–8850 m with heterogeneous topography and distinct climatic zones. The country is considered a biodiversity hotspot, with nearly a quarter of the land area located in protected areas. Nepal and the surrounding Himalayan region are particularly vulnerable to climate change because of their abrupt ecological and climatic transitions. Tens of millions of people rely on the region’s ecosystem services, and observed and modeled warming trends predict increased climate extremes in the Himalayas. To study the ecological impacts of climate change in Nepal and inform adaptation planning, we review the literature on past, present, and predicted future climatic changes and their impacts on ecological diversity in Nepal. We found few studies focusing on organisms, while research on species and communities was more common. Most studies document or predict species range shifts and changes in community composition. Results of these few investigations highlight major lacunae in research regarding the effects of changing climate on species comprising the Himalayan biota. Further empirical work is needed at all levels of biological organization to build on information regarding direct ecological impacts of climatic changes in the region. Countries face an ever-increasing threat of climate change, and Nepal has strong physiographic, elevational, and climatic gradients that could provide a useful model for studying the effects of climate change on a mountainous, and highly biodiverse, area

Table_4_A pro-oxidant combination of resveratrol and copper down-regulates hallmarks of cancer and immune checkpoints in patients with advanced oral cancer: Results of an exploratory study (RESCU 004).docx

BackgroundOur earlier studies have shown that cell-free chromatin particles (cfChPs) that are released from dying cancer cells are readily internalised by bystander cells leading to activation of two hallmarks of cancer viz. genome instability and inflammation. These hallmarks could be down-regulated by deactivating cfChPs via medium of oxygen radicals generated upon admixing small quantities of the nutraceuticals resveratrol (R) and copper (Cu). In this exploratory study, we investigated whether oral administration of R and Cu (R-Cu) would down-regulate the hallmarks of cancer and immune checkpoints in advanced squamous cell carcinoma of oral cavity (OSCC).Patients and methodsThe study comprised of 25 patients divided into 5 equal groups. Five patients acted as controls; the remaining 20 were given R-Cu in four escalating doses. The lowest dose of R-Cu was 5.6mg and 560ng respectively, and the highest dose was 500mg and 5mg respectively. An initial biopsy was taken from patients at first presentation, and a second biopsy was taken 2 weeks later on the operating table. R-Cu was administered orally twice daily in the intervening period. Confocal microscopy was performed on tumour sections after fluorescent immuno-staining with anti-DNA and anti-histone antibodies to detect presence of cfChPs in the tumour micro-environment (TME). Immunofluorescence analysis was performed for 23 biomarkers representing the 10 Hallmarks of cancer, including 5 immune checkpoints, defined by Hanahan and Weinberg.ResultsConfocal microscopy detected copious presence of cfChPs in TME of OSCC, which were eradicated/deactivated following two-week treatment with R-Cu. Eradication of cfChPs from TME was associated with marked down-regulation of 21/23 biomarkers, including the five immune checkpoints. The lower two doses of R-Cu were more effective than the higher doses. No adverse effects attributable to R-Cu were observed.ConclusionThese results suggest that cfChPs released into TME from dying cancer cells are global instigators for cancer hallmarks and immune checkpoints in surviving cancer cells. The ability of R-Cu to deactivate cfChPs raises the prospect of a novel and non-toxic form of cancer treatment which sans killing of cancer cells, and instead induces healing by down-regulating cancer hallmarks and immune check-points.Clinical Trial Registrationhttp://ctri.nic.in/Clinicaltrials/pmaindet2.php?trialid=19801&EncHid=&userName=CTRI/2018/03/012459.</p

Image_4_A pro-oxidant combination of resveratrol and copper down-regulates hallmarks of cancer and immune checkpoints in patients with advanced oral cancer: Results of an exploratory study (RESCU 004).png

BackgroundOur earlier studies have shown that cell-free chromatin particles (cfChPs) that are released from dying cancer cells are readily internalised by bystander cells leading to activation of two hallmarks of cancer viz. genome instability and inflammation. These hallmarks could be down-regulated by deactivating cfChPs via medium of oxygen radicals generated upon admixing small quantities of the nutraceuticals resveratrol (R) and copper (Cu). In this exploratory study, we investigated whether oral administration of R and Cu (R-Cu) would down-regulate the hallmarks of cancer and immune checkpoints in advanced squamous cell carcinoma of oral cavity (OSCC).Patients and methodsThe study comprised of 25 patients divided into 5 equal groups. Five patients acted as controls; the remaining 20 were given R-Cu in four escalating doses. The lowest dose of R-Cu was 5.6mg and 560ng respectively, and the highest dose was 500mg and 5mg respectively. An initial biopsy was taken from patients at first presentation, and a second biopsy was taken 2 weeks later on the operating table. R-Cu was administered orally twice daily in the intervening period. Confocal microscopy was performed on tumour sections after fluorescent immuno-staining with anti-DNA and anti-histone antibodies to detect presence of cfChPs in the tumour micro-environment (TME). Immunofluorescence analysis was performed for 23 biomarkers representing the 10 Hallmarks of cancer, including 5 immune checkpoints, defined by Hanahan and Weinberg.ResultsConfocal microscopy detected copious presence of cfChPs in TME of OSCC, which were eradicated/deactivated following two-week treatment with R-Cu. Eradication of cfChPs from TME was associated with marked down-regulation of 21/23 biomarkers, including the five immune checkpoints. The lower two doses of R-Cu were more effective than the higher doses. No adverse effects attributable to R-Cu were observed.ConclusionThese results suggest that cfChPs released into TME from dying cancer cells are global instigators for cancer hallmarks and immune checkpoints in surviving cancer cells. The ability of R-Cu to deactivate cfChPs raises the prospect of a novel and non-toxic form of cancer treatment which sans killing of cancer cells, and instead induces healing by down-regulating cancer hallmarks and immune check-points.Clinical Trial Registrationhttp://ctri.nic.in/Clinicaltrials/pmaindet2.php?trialid=19801&EncHid=&userName=CTRI/2018/03/012459.</p