Seed Germination and Seedling Growth in Solanum Species to Water Stress under in vitro Conditions

A study on seed germination and seedling growth was conducted with five cultivars of Solanum melongena L. (cvs. Arka Nidhi, B.P.L.H.-1, Arka Neelakanth, Arka Keshav and Mattu Gulla) and a wild species Solanum macrocarpon L. under different levels of osmotic potential induced by polyethylene glycol (PEG 8000). Germination declined progressively in response to decreasing (more negative) water potential, and no germination was found beyond - 0.4MPa in any of the cultivars/species. Except for cvs. Arka Nidhi, B.P.L.H.-1 and Mattu Gulla, no germination was seen at -0.4MPa Cultivar Arka Neelkanth failed to germinate under any of the osmotic concentrations tested. Response in term of root growth was better in Arka Neelkanth, followed by Arka Nidhi and B.P.L.H.-1, upto transfer from different levels of osmotic potential to Control (0MPa). Germination of primed seeds within 24h indicates that many processes leading to normal germination would have been completed during the priming process itself. In contrast to germination, growth extension in radicle was less sensitive to water stress

Compendium of Climate-smart Agriculture Technologies and Practices

Nepal is a vulnerable country to the impacts of climate change. The adverse effects of climate change have decreased agricultural production and productivity. Therefore, technologies and strategies to develop resilience agriculture and increased agriculture productivity are urgently needed to create climate-smart technologies and help the existing technologies to adapt to climate change. This compendium presents climate-smart agriculture (CSA) technologies and interventions for enhancing food security with adaptation and mitigation as co-benefits. CSA involves smart farming practices and strategies that help develop resilience agriculture, increase crop and livestock productivity, reduce greenhouse gas emissions, and enhance food security goals. This compendium has been developed for extension workers to support up-scaling climate-smart technologies and build climate resilience villages that enhance food, nutrition, and income, mainly for marginal communities striving in marginal areas

Breeding tomato (Solanum lycopersicum L.) for resistance to biotic and abiotic stresses

Tomato (Solanum lycopersicum L.) is an important vegetable crop cultivated in the tropical and sub-tropical regions of the world. Low productivity in India is due to occurrence of both biotic and abiotic stresses. Among the biotic stresses, tomato leaf curl disease, bacterial wilt, early blight and Groundnut Bud Necrosis Virus disease have become serious production constraints causing considerable yield loss in the major tomato growing areas of the country. Adoption of multiple disease resistant varieties or F1 hybrids would be the most appropriate way to address these diseases. At ICAR-IIHR, Bengaluru systematic breeding strategies were employed to pyramid genes for resistance to early blight, bacterial wilt and tomato leaf curl diseases and to develop advanced breeding lines& F1 hybrids with triple disease resistance. Stable source of resistance to early blight and bi-partite begomo-virus (Tomato Leaf Curl New Delhi Virus) has been identified in Solanum habrochaites LA-1777. Validation with molecular markers linked to tomato leaf curl virus resistance revealed that LA-1777 carryTy2 and other putative resistant genes. Several high yielding dual purpose hybrids were also developed for fresh market and processing with high level of resistance to multiple diseases. Cherry tomato lines have also been bred for high TSS, total carotenoids, total phenols, flavonoids, vitamin C, acidity and lycopene content. IIHR-249-1, IIHR-2101 (Solanum habrochaites LA-1777), IIHR- 2866 and IIHR-2864 recorded high values for quality parameters like total carotenoids, lycopene, vitamin C, total phenols, flavonoids and TSS. Drought tolerant root stock has been developed by an interspecific cross between S. habrochaites LA-1777 and S. lycopersicum (15 SB SB). Resistant sources have also been identified against Tuta absoluta, a serious insect pest reported from major tomato growing areas in the country in recent time. High temperature tolerant breeding lines are in pipe line

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

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Not AvailablePhenotyping approaches, using high throughput imaging techniques, are being adopted over the traditional methodologies which are manpower intensive, time consuming and low throughput. However, the effectiveness of high throughput plant phenotyping through imaging in plant phenomics facility essentially requires establishing relationship between plant areas quantified through imaging and the actual biomass. The present study was conducted with an aim to standardise the methodology for digital quantification of tomato biomass using plant phenomics facility. A strong linear relationship was observed between the actual tomato plant fresh mass, digital biomass and projected shoot area. The correlations between plant fresh mass, plant digital biomass and projected shoot area were highly significant at 30, 45 and 60 days after transplanting, but at 75 days no correlation was observed.Hence, the present study clearly demonstrated that the growth of tomato plants could be monitored through digital imaging using either projected shoot area or digital biomass till 60 days after transplanting across genotypes for high throughput phenotyping.ICAR-NICR

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Not AvailableBanana is a non- model crop plant, and one of the most important crops in the tropics and sub tropics. Heat stress is the major abiotic stress affecting banana crop production because of its long growth period and is likely to become a threat due to global warming. To understand an acquired thermotolerance phenomenon at the molecular level, the RNA-seq approach was employed by adapting TIR method. A total of 136.38 million high quality reads were assembled. Differentially expressed genes under induction (I) was 3936, I + L was 2268 and lethal stress was 907 compared to control. Gene ontology and DGE analysis showed that genes related to heat shock factors, heat shock proteins, stress associated proteins, ROS scavenging, fatty acid metabolism, protein modification were significantly up regulated during induction, thus preparing the organism or tissue at molecular and cellular level for acquired thermotolerance. KEGG pathway analysis revealed the significant enrichment of pathways involved in protein processing, MAPK signaling and HSPs which indicates that these processes are conserved and involved in thermo tolerance. Thus, this study provides insights into the acquired thermotolerance phenomena in plants especially banana.ICAR-NICR

Evaluation of Solanum lycopersicum L. genotypes for high temperature stress tolerance employing temperature induction response technique

680-689Solanum lycopersicum L., an important vegetable crop (Tomato) in most regions of the world, is affected by high temperature stress at various stages of its growth. Likely increase in periodicity of high temperature episodes under climate change conditions would further affect the tomato production. To sustain productivity and yields under climate change situations, there is an urgent need to develop suitable cultivars as an adaptation strategy. For this endeavour, identification of high temperature tolerant lines for crop improvement is a prerequisite. Hence in the present study, temperature induction response (TIR) technique was employed to evaluate thermotolerance in 52 tomato genotypes. Two day old seedlings were subjected to an initial induction treatment i.e., gradual temperature increase from 33-43°C for 3 h followed by 50°C for 3 h as challenging temperature. Wide variability was seen for seedling survival and vigour. In genotypes IIHR-2202, IIHR-2836 and IIHR-2841 seedling survival was as high as 90%, while H-329, H-371, IIHR-2745 and H-335 showed least reduction in growth and also had better seedling vigour index. Of the 52 genotypes, 21 were identified as tolerant, 12 moderately tolerant and 19 susceptible. This TIR technique is simple, quick and less expensive than whole plant screening. Hence, it could be used by plant physiologists and plant breeders for screening seedlings at an early stage in the phenotyping and crop improvement programmes

Seed germination and seedling growth in Solanaceous species to water stress under in vitro conditions

Not AvailableA study on seed germination and seedling growth was conducted with five cultivars of Solanum melongena L. (cvs. Arka Nidhi, B.P.L.H.-1, Arka Neelakanth, Arka Keshav and Mattu Gulla) and a wild species Solanum macrocarpon L. under different levels of osmotic potential induced by polyethylene glycol (PEG 8000). Germination declined progressively in response to decreasing (more negative) water potential, and no germination was found beyond -0.4MPa in any of the cultivars/species. Except for cvs. Arka Nidhi, B.P.L.H.-1 and Mattu Gulla, no germination was seen at -0.4MPa Cultivar Arka Neelkanth failed to germinate under any of the osmotic concentrations tested. Response in term of root growth was better in Arka Neelkanth, followed by Arka Nidhi and B.P.L.H.-1, upto transfer from different levels of osmotic potential to Control (0MPa). Germination of primed seeds within 24h indicates that many processes leading to normal germination would have been completed during the priming process itself. In contrast to germination, growth extension in radicle was less sensitive to water stress.ICAR under NICRA projec

Response of brinjal genotypes to drought and flooding stress

Not AvailableA study was conducted to evaluate the plant responses to drought and flooding in eight genotypes of brinjal (Solanum melongena L. cvs. I.I.H.R.3, BMG-1, BPLH-1, Arka Kesav, Arka Neelkanth, Arka Nidhi, Arka Shirish, Mattu Gulla) and a wild type Solanum macrocarpon. The results indicated the greater sensitivity of stomata under flooding compared to drought in brinjal genotypes. Genotype BMG- 1 performed photosynthetically better under drought, while under flooding Arka Nidhi and Arka Keshav has shown higher photosynthetic rate. The closure of stomata in drought was gradual, while during flooding the closure was quick as shown the differential response of stomatal conductance in brinjal genotypes under these two conditions. During flooding, the better water balance was maintained by Arka Shirish and Arka Neelkanth, while during drought, Arka Keshav and Arka Neelkanth had the better water balance as indicated by greater RWC and osmotic potential values. The study further envisage that apart from the plant performance during stress, the recovery potential is an important aspects and particularly in flooding where the recovery process seems to be very slow as compared to drought. The better recovery after releasing flooding was found in Arka Keshav, Arka Neelkanth, IIHR-3 and BPLH-1 followed by S. macrocarpon and Mattu Gulla, while after releasing the drought stress, better recovery was observed in BMG-1, Arka Shirish, Arka Neelkanth, Arka Nidhi, S, macrocarpon, and BPLH-1.ICAR under NICRA projec