STUDY OF PREVALENCE AND ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF BACTERIAL ISOLATES IN A TERTIARY CARE HOSPITAL

Objective: Many human illnesses are bacterial in origin which can be treated with appropriate antibiotics and selection of these is mostly based on culture and sensitivity. The problem of antimicrobial resistance has burgeoned throughout the world both in inpatients and outpatients. We must work together to preserve the power of antimicrobials so as to use these effectively in treating diseases. The aim of the study is to find out the prevalence of infection and sensitivity pattern among bacterial pathogens in a tertiary care hospital.Methods: This retrospective study was carried out in Vinayaka Missions Medical College and Hospital, Karaikal, Puducherry (U. T) to study the culture and sensitivity pattern of clinical isolates from blood, urine, sputum, wound, ear/throat swab for one year (June 2012- June 2013). The positive cultures and their antibiotic susceptibility testing were performed under the guidelines of Clinical and Laboratory Standard Institute (CLSI). The lab data from Microbiology department were utilized and filled in a proforma and analyzed.Results: Out of 788 samples, 296 were culture positive. Isolated bacteria were mostly Gram negative organisms (GNO) of which Klebsiella (41.55%) was commonly followed by Pseudomonas (15.20%), Escherichia coli(4.05%) and Proteus (3.71%). Among Gram positive organisms (GPO) Staphylococcus (35.47%) was common. Imipenem (100%), Gentamycin (90.20%), Amikacin (89.14%), were the antimicrobials most sensitive for GNO, while Ceftriaxone (100%), Cefotaxime(100%),Nitrofurantoin(96.15%) and Linezolid(92.13%) were most sensitive for GPO. Widespread resistance to Nalidixic acid (99%),Ampicillin (85.90%), Cefuroxime (75%) is seen in both groups, while Cefazolin(79.25%) and Norfloxacin(83%) was noted for GNO, resistance to Amoxicillin with clavulanic acid (81.66%) and Imipenem(55.55%) was noted for GPO.Conclusion: The technical abilities to detect novel, resistant mechanisms and to overcome the microbial resistance has to be improved. Antibiotic policies agreed among clinicians, microbiologists and pharmacologists will guide good prescribing, provide maximum coverage for treating infections and ensure antibiotic cycling.Â

An interesting rare case of spontaneous pregnancy with imperforate hymen and high transverse vaginal septum with bicornuate uterus

Congenital anatomic disorders of the female reproductive tract may result from genetic mutation, developmental arrest and abnormal hormonal exposure during embryonic development. These anomalies have a huge impact on the woman’s menstrual, sexual and reproductive functions. Pregnancy with these anatomic disorders is quite challenging. Here we present a rare interesting case of spontaneous pregnancy with multiple anatomic disorders namely- imperforate hymen, high transverse vaginal septum and bicornuate uterus

The Removal of Random Valued Impulse Noise Using Contrast Enhancement and Decision Based Filter

Digital images are transmitted in noisy environment and it will frequently affected by impulse noise .To remove this noise from the image is a fundamental problem of image processing. There are various types of noise in an image especially salt and pepper noise and random valued impulse noise. This paper introduces a new filtering scheme based on contrast enhancement filter and decision based filter for removing the random valued impulse noise. The application of a nonlinear function to increasing the difference between noise pixels and noise-free and results in efficient detection of noisy pixels. As the performance of a filtering system, in general, depends on the number of iterations used, the effective stopping criterion based on noisy image characteristics to determine the number of iterations is also proposed. This proposed method removes only the corrupted pixel by its neighboring pixel values. As a result of this, the proposed method removes the noise effectively and preserves the edges without any loss up to 80% of noise level

EXPLORING THE COMPETENCE OF PHYTOCHEMICAL COMPOUNDS TO COMBAT ANTHRACNOSE DISEASE OF CUCUMBER -AN IN SILICO APPROACH

Objective: Anthracnose is one of the diseases of cucumber caused by the fungus Colletotrichum lagenarium. The enzymes of fungal melanin biosynthesis pathway act as a potential target for the synthetic fungicides. The present study aims to identify the rational phytochemical compound that exhibits inhibitory activity towards a key enzyme scytalone dehydratase (SCD) of melanin biosynthesis pathway. Methods: Phytochemical compounds with antifungal activity were screened from the conventionally used medicinal plants and docked with the target enzyme SCD. Results: The interaction amongst phytocompounds and the target SCD were evaluated. Five out of 20 compounds exhibited higher binding affinity compared to that of synthetic fungicide carpropamid. Conclusion: The current study revealed that the compounds exhibiting significant interaction with SCD might act as an efficient fungicide for the control of anthracnose disease of cucumber

Fast Subpixel Full Search Motion Estimation

Motion estimation is one of the most important part in video coding, where only the difference between the current and reference frames will be coded by the encoder.There are many advancements happening in motion estimation techniques. The proposed algorithm provides high precision matching and even reduces the errors during compensation. The algorithm also reduces the computation time when compared to traditional Block matching techniques. It mainly aims at the motion estimation with subpixelaccuracy without interpolation, it is the combination of Block matching and the optical flow method.Fast computation may be evaluated by experimental results while even motion vectors are more accurate reducing the PSNR

Life cycle and morphometry of Rugose spiraling whitefly, Aleyrodicus rugioperculatus Martin (Hemiptera: Aleyrodidae) on coconut

The present study investigated the biology and morphometric analysis of rugose spiralling whitefly (RSW), Aleyrodicus rugioperculatus on coconut under mini net house condition at Department of Agricultural Entomology, Tamil Nadu Agricultural University-Coimbatore during 2019-2020. The biology of rugose spiralling whitefly was studied by clip cage method and morphometrics were done using Leica image analyser. Total lifecycle of rugose spiraling whitefly was 56.23 ± 2.20 days. Developmental period of egg, nymphal, pupal and adult period was 8.47 ± 0.26, 17.46 ± 0.76, 10.30 ± 0.29 and 20.00 ± 1.00 days, respectively. In morphometrics, Length and width of egg (0.31 ± 0.01 mm and 0.11± 0.02 mm), nymphal (0.94 ± 0.01 mm and 0.82 ± 0.01 mm), pupal (1.23 ± 0.01 mm and 1.00 ± 0.01 mm) was recorded. A nymphal parasitoid, Encarsia guadeloupae can be potential natural enemy for effective management of rugose spiraling whitefly.

Impact of Lysinibacillus macroides, a potential plant growth promoting rhizobacteria on growth, yield and nutritional value of tomato Plant (Solanum lycopersicum L. f1 hybrid Sachriya)

Plant growth promoting bacteria enhance the growth in plants by solubilizing insoluble minerals, producing phytohormones and by secreting enzymes that resist pathogen attack. The present study was aimed at identifying the potential of Lysinibacillus macroides isolated from pea plant possessing rich microbial rhizobiome diversity in promoting the growth of tomato plant (Solanum lycopersicum L). Potential of L. macroides in the promotion of S. lycopersicum L. growth by increased shoot length, terminal leaf length and breadth was assessed. Anatomical sectioning of stem and root revealed no varied cellular pattern indicating that the supplemented bioculture is not toxic to S. lycopersicum. Plantlets treated with L. macroides along with organic compost showed an increased total phenol content (17.58±0.4 mg/g) compared to control samples (12.44±0.41 mg/g). Carbohydrate content was noticed to be around 1.3 folds higher in the L. macroides plus compost mixture supplemented slots compared to control sample. Significant increase in shoot length was evident in the L. macroides plus compost supplied slots (23.4±2.7 cm). Plant growth promoting properties might be due to the nitrogen fixing activity of the bacteria which enrich the soil composition along with the nutrients supplied by the organic compost. Rich microbial rhizobiome diversity in pea plant and the usage of L. macroides from a non-conventional source improves the diversity of the available PGPR for agricultural practices. Further research is needed to detect the mechanism of growth promotion and to explore the plant microbe interaction pathway