Helicteres isora L: regeneration through meristem culture

Helicteres isora Linn (Sterculiaceae) is an endangered plant species of medicinal importance. Rapid regeneration of this plant was achieved via shoot tip explants with highest response of shoot induction (100%) on MS basal medium supplemented with 12 μM 6-ben-zylaminopurine (BAP) and 16 μM kinetin (Kin) alone. This combination of phytohormones was found suitable for complete plant regeneration through meristem culture. The developed method can be successfully employed for large-scale regeneration and conservation of this medicinal plant

The Future: Machine learning

Learning is a process or activity of understanding and improving one’s then it may be human or machineability to perform a operations efficiently. Machine learning is one of sub-field of computer science, which enables computers to learn and analyze thinks without being or writing explicitly programmed. It basically evolved from AI(artificial intelligence) via pattern recognition and computational learning theory. It also explores the area of algorithms, which can make high end predictions on data. Currently it deployed in a wide range of computing tasks, where designing efficient algorithms and programs becomes rather difficult, such as email spam filtering, optical character recognition, search engine improvement etc.Advance machine learning will basically concentrate on modeling, generation, and prediction of multiple inter-dependent variables

Effect of sugars on production of β-sitosterol from in vitro callus culture of Boerhaavia diffusa L.

The establishment of friable callus was achieved on Murashige and Skoog (MS) medium fortified with 18 μM of 2,4-dichlorophenoxyacetic acid in combination with 2 μM of 6-benzylaminopurine (BAP). The effect of different sugars such as 3% sucrose, 3% glucose and 3% galactose on the growth of callus tissue and β-sitosterol accumulation was tested. Quantification of β-sitosterol was carried out with the help of high performance liquid chromatography. High growth of callus tissue was observed on MS medium, containing sucrose and glucose as carbon sources and maximum β-sitosterol content was observed in 60 days old callus tissue with 3% glucose as carbon source

Motor hyperactivity of the iron-deficient rat - an animal model of restless legs syndrome.

BackgroundAbnormal striatal dopamine transmission has been hypothesized to cause restless legs syndrome. Dopaminergic drugs are commonly used to treat restless legs syndrome. However, they cause adverse effects with long-term use. An animal model would allow the systematic testing of potential therapeutic drugs. A high prevalence of restless legs syndrome has been reported in iron-deficient anemic patients. We hypothesized that the iron-deficient animal would exhibit signs similar to those in restless legs syndrome patients.MethodsAfter baseline polysomnographic recordings, iron-deficient rats received pramipexole injection. Then, iron-deficient rats were fed a standard rodent diet, and polysomnographic recording were performed for 2 days each week for 4 weeks.ResultsIron-deficient rats have low hematocrit levels and show signs of restless legs syndrome: sleep fragmentation and periodic leg movements in wake and in slow-wave sleep. Iron-deficient rats had a positive response to pramipexole treatment. After the iron-deficient rats were fed the standard rodent diet, hematocrit returned to normal levels, and sleep quality improved, with increased average duration of wake and slow-wave sleep episodes. Periodic leg movements decreased during both waking and sleep. Hematocrit levels positively correlated with the average duration of episodes in wake and in slow-wave sleep and negatively correlated with periodic leg movements in wake and in sleep. Western blot analysis showed that striatal dopamine transporter levels were higher in iron-deficient rats.ConclusionsThe iron-deficient rat is a useful animal model of iron-deficient anemic restless legs syndrome. © 2017 International Parkinson and Movement Disorder Society

Occurrence of Some Freshwater Rotifers from Visapur Dam, Ahmednagar, Maharashtra, India

Rotifers from a reservoir (Vispaur Dam, Ahmednagar, Maharashtra) were studied for a period of seven months. Samples were collected using a 50-micron mesh size plankton net, preserved in 4% formalin and identified under a compound microscope using standard rotifer literature.  Nineteen species belonging to 9 families were seen in the studied samples, with family Brachionidae being species rich with 9 species. The genus Brachionus was well represented with 5 species. The results are compared with studies done on rotifers from reservoirs all over Maharashtra state. Detailed studies in the future are required for more exact estimate of rotifer diversity. Detailed studies in future will add to the checklist of rotifers from the studied waterbody

Spectral-domain optical coherence tomography in choroidal rupture following firecracker injury

In India, firecrackers are an integral and essential part of the celebrations. Injuries from firecrackers can lead to serious and irreparable visual damage. We report a case of choroidal rupture following injury caused by firecracker and correlate its optical coherence tomography findings. As ocular firecracker injuries result in significant morbidity, public education regarding proper use of firecrackers may help in reducing the incidence of ocular injuries

Sterculiaceae – A critical appraisal on plant tissue culture studies in medicinally important plants

The members of Sterculiaceae are valuable source of different kinds of useful products like wood, medicine, fiber, firewood and timber suitable for furniture, as well as decorative plants, pharmaceutical products and aestic items. In recent years the relevance of plant tissue culture methods has gained thrust to meet the growing demands for pharmaceutical industries since the genetic diversity of medicinal plants is decreasing hap hazardously. Conventional propagation and high demand of planting material are the major constraints for the large scale multiplication of medicinal species which can be met economically and efficiently in short span of time by in vitro propagation. Micropropagation is an alternative means of propagation that can be engaged in the conservation of flora in relatively shorter time. Tissue culture is useful for multiplying and conserving the useful species which are difficult to regenerate and thus can be saved from extinction. There is an only partial advancement explored at the research level to suggest tissue culture studies in Sterculiaceae. Conclusively, this appraisal is the first of its kind which emphasizes the procedures available for in vitro propagation along with some of the remarkable achievements carried in some pharmaceutically and commercially important plants by in vitro culture techniques of plants from this family Keywords: Pharmaceutical, medicinal, propagation, in vitro, Sterculiacea