Genetic characterization of Toxoplasma gondii isolates from pigs intended for human consumption in Brazil

AbstractThis study genetically Toxoplasma gondii isolates obtained from pigs intended for human consumption in northeastern Brazil; multilocus PCR-RFLP and sequencing techniques were utilized. Bioassays were conducted using the brain and tongue of 20 pig heads purchased at butcher shops in the city of Ilheus, Bahia, Brazil. Overall, 11 T. gondii isolates designated TgPgBr06-16 were identified. Application of multilocus PCR-RFLP with seven molecular markers (SAG1, SAG2, SAG3, BTUB, C22-8, PK1 and Apico) identified six different genotypes. Isolates TgPgBr 06, 08, 11, 12, 14 and 15 were indistinguishable by this technique, forming a single genotype; the remaining isolates were characterized as distinct genotypes. However, when five genetic markers (SAG1, SAG2, SAG3, BTUB and c22-8) were employed in multilocus PCR-sequencing, all eleven strains of T. gondii were shown to be different. All isolates differed from Type I, II and III clonal genotypes using both genotyping techniques. These results demonstrate that the multilocus PCR-RFLP assay underestimated the true diversity of the T. gondii population in this study. Thus, DNA sequencing is the preferred technique to infer the genetic diversity and population structure of T. gondii strains from Brazil. Moreover, it is necessary to develop new molecular markers to group and characterize atypical T. gondii isolates from South America

Use of Cellulosic Materials as Dye Adsorbents — A Prospective Study

Cellulose is the most abundant biopolymer of nature, and it is widely used in the synthesis of new materials as well as in the adsorption of dye. This study reports a literature review (articles) and technology review (patents) about publications and product invention, which contain information on the use of cellulose on the adsorption of dyes in the period 2004–2014. For this work, research database and keywords were used to find articles and patents related to the subject under review. Specific words were used to find articles and patents related to the subject under review. After a demanding research, 1 patent and 23 articles that contain the words “cellulose,” “dye,” and “adsorption or sorption” in their titles were assessed, and annual evolution studies were performed for publications and countries that publish more

Chemical Functionalization of Cellulosic Materials — Main Reactions and Applications in the Contaminants Removal of Aqueous Medium

The cellulose is the most abundant biopolymer in the world and presents a higher chemical variability for presence of several hydroxyl groups. These hydroxyl groups allow surface modification of biomaterials, with insertion of several chemical groups which change cellulose characteristics. This natural biopolymer and its derivatives have been used a lot as adsorbent, from several contaminants of aqueous medium due to biocompatibility, chemical degradability, and variability. Therefore, this chapter has the objective to review the literature about several cellulose surfaces or cellulosic material (incorporation of carboxymethyl, phosphorus, carboxyl, amines, and sulfur), presenting the main characteristics of reactions and showing its adsorption in application of aqueous medium (metals, dyes, and drugs), locating the main interactions between biomaterial/contaminant

Ictiofauna Dos Enclaves De Floresta úmida Nos Planaltos Da Ibiapaba E Do Araripe, Nordeste Do Brasil

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Humid highland forest enclaves are remnants of Atlantic Forest found in tablelands within the Caatinga biome (Northeastern Brazil), which emerged during interglacial periods in the Pleistocene. These ecosystems have a highly diverse and endemic fish fauna. Most earlier surveys have focused on the tableland of Borborema (Pernambuco and Paraíba States). In this study we surveyed the fish fauna of the humid forest enclaves in the tablelands of Ibiapaba and Araripe, based on samples collected in the rainy season (March and April) between 2009 and 2014. The 45 sampling points covered rivers, streams and reservoirs in five river basins belonging to three ecoregions. The species were listed according to drainage divide, and endemism was determined for each ecoregion and for the Caatinga. Our area was more species-rich (n=59) than Borborema (n=27). The samples included five introduced species and 29 species endemic to the Caatinga (49.1% of the sampled species). The distribution of Parotocinclus haroldoi was expanded to the Mid-Northeastern Caatinga ecoregion (Timonha river basin, Ceará State). Our study intends to make a significant contribution to current knowledge of the ichthyofauna in humid highland forest enclaves of semiarid Northeastern Brazil, identified as a priority in the conservation of the biodiversity in the Caatinga. © 2016, Universidade Estadual de Campinas UNICAMP. All rights reserved.164457463/2012-0, CNPq, Conselho Nacional de Desenvolvimento Científico e Tecnológico552009/2011-3, CNPq, Conselho Nacional de Desenvolvimento Científico e Tecnológico552086/2011-8, CNPq, Conselho Nacional de Desenvolvimento Científico e TecnológicoConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

EPIDEMIOLOGICAL PROFILE OF ARBOVIROSIS IN THE STATE OF MARANHÃO: DENGUE FROM 2010 TO 2020

Dengue is an arbovirus considered a growing problem in relation to public health worldwide. With this, the objective of this work is to describe, in a retrospective and analytical way, the epidemiological profile and the spatial distribution of dengue cases in the state of Maranhão between the years 2010 to 2020. This quantitative and retrospective study used secondary data provided by the State Department of Health (SES-MA) of confirmed and notified cases of the disease in a time frame between 2010 and 2020. In addition, geoprocessing techniques were also used to spatialize the data. The results indicate that there were oscillations in relation to confirmed and reported cases throughout this period in the state, especially in the north and southwest regions. Thus, Maranhão reveals a critical state in relation to the occurrence of the disease, which is possibly associated with environmental factors, infrastructure and lack of public policies related to basic sanitation.info:eu-repo/semantics/publishedVersio