Auswirkungen der Softwaregestaltung Vorstudie zur Technikfolgenabschaetzung

Available from TIB Hannover: F94B530+a / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEBundesministerium fuer Forschung und Technologie (BMFT), Bonn (Germany)DEGerman

Comparison of Different Approaches To Quantify Staphylococcus aureus Cells by Real-Time Quantitative PCR and Application of This Technique for Examination of Cheese

Two different real-time quantitative PCR (RTQ-PCR) approaches were applied for PCR-based quantification of Staphylococcus aureus cells by targeting the thermonuclease (nuc) gene. Purified DNA extracts from pure cultures of S. aureus were quantified in a LightCycler system using SYBR Green I. Quantification proved to be less sensitive (60 nuc gene copies/μl) than using a fluorigenic TaqMan probe (6 nuc gene copies/μl). Comparison of the LightCycler system and the well-established ABI Prism 7700 SDS with TaqMan probes revealed no statistically significant differences with respect to sensitivity and reproducibility. Application of the RTQ-PCR assay to quantify S. aureus cells in artificially contaminated cheeses of different types achieved sensitivities from 1.5 × 10(2) to 6.4 × 10(2) copies of the nuc gene/2 g, depending on the cheese matrix. The coefficients of correlation between log CFU and nuc gene copy numbers ranged from 0.979 to 0.998, thus enabling calculation of the number of CFU of S. aureus in cheese by performing RTQ-PCR

ROBL - a CRG beamline for radiochemistry and materials research at the ESRF

Der Bericht beschreibt die Rossendorfer Beamline (ROBL), die vom Forschungszentrum Rossendorf an der ESRF errichtet wurde. ROBL besteht aus zwei unabhaengigen Messplaetzen: einem kleinen radiochemischen Labor fuer Roentgen-Adsorptionsspektroskopie an offenen radioaktiven Proben und einem Vielzweckmessplatz fuer Materialuntersuchungen insbesondere mit Roentgendiffraktion und Reflektometrie. Der Radiochemie-Messplatz ist eine weltweit unikale Einrichtung an einer modernen Synchrontronstrahlungsquelle. (orig.)The paper describes the Rossendorf Beamline (ROBL) built by the Forschungszentrum Rossendorf at th ESRF. ROBL comprises two different and independently operating experimental stations: a radiochemistry laboratory for X-ray absorption spectroscopy of non-sealed radioactive samples and a general purpose materials research station for X-ray diffraction and reflectometry mainly of thin films and interfaces modified by ion beam techniques. The radiochemistry set-up is worldwide a unique installation at a modern synchrotron radiation source. (orig.)Available from TIB Hannover: RR 1847(256) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEDEGerman