FGF-21 levels in polyuria-polydipsia syndrome

The pathomechanism of primary polydipsia is poorly understood. Recent animal data reported a connection between fibroblast growth factor 21 (FGF-21) and elevated fluid intake independently of hormonal control by the hormone arginine-vasopressin (AVP) and osmotic stimulation. We therefore compared circulating FGF-21 levels in patients with primary polydipsia to patients with AVP deficiency (central diabetes insipidus) and healthy volunteers. In this prospective cohort study, we analyzed FGF-21 levels of 20 patients with primary polydipsia, 20 patients with central diabetes insipidus and 20 healthy volunteers before and after stimulation with hypertonic saline infusion targeting a plasma sodium level >= 150 mmol/L. The primary outcome was the difference in FGF-21 levels between the three groups. Baseline characteristics were similar between the groups except for patients with central diabetes insipidus being heavier. There was no difference in baseline FGF-21 levels between patients with primary polydipsia and healthy volunteers (122 pg/mL (52,277) vs 193 pg/mL (48,301), but higher levels in patients with central diabetes insipidus were observed (306 pg/mL (114,484);P=0.037). However, this was not confirmed in a multivariate linear regression analysis after adjusting for age, sex, BMI and smoking status. Osmotic stimulation did not affect FGF-21 levels in either group (difference to baseline: primary polydipsia -23 pg/mL (-43, 22);central diabetes insipidus 17 pg/mL (-76, 88);healthy volunteers -6 pg/mL (-68, 22);P=0.45). To conclude, FGF-21 levels are not increased in patients with primary polydipsia as compared to central diabetes insipidus or healthy volunteers. FGF-21 therefore does not seem to be causal of elevated fluid intake in these patients

Die Dauerausstellung im Jüdischen Museum

Am neunten September wurde die mit Spannung erwartete Dauerausstellung im Jüdischen Museum Berlin eröffnet. Sie erstreckt sich über zwei Etagen des vom us-amerikanischen Architekten Daniel Libeskind entworfenen, neu geschaffenen Museumsgebäudes auf einer Fläche von mehreren tausend Quadratmetern, chronologisch der Geschichte jüdischen Lebens in Deutschland folgend: Kulturhistorisch perspektiviert und mitunter repräsentativ personalisiert führt ein Parcours im obersten Gebäudegeschoss von frühen jüdischen Spuren im alten Germanien und den blühenden mittelalterlichen Gemeinden über das Leben der Händlerin und Geschäftsfrau Glikl bas Juda Leib und dem sogenannter "Land-und Hofjuden", weiter zur Bedeutung von "Bürgertum und Familie" und schließlich zum "Religiösen Leben" in Tradition und Wandel ..

Effekt der Glycosylierung von CD16 (Fc[gamma]RIII) auf das IgG-Bindungsverhalten

[no abstract

Mechanistische Untersuchung multivalenter Kohlenhydrat-Protein-Wechselwirkungen durch EPR-Spektroskopie

Durch Abstandsmessungen mit Spinsonden-EPR-Spektroskopie gelang der direkte Nachweis multivalenter Protein-Ligand-Interaktionen in Lösung. Man erhält ein detailliertes Bild des Mechanismus der Bindung divalenter Liganden an ein Lectin. Chelatbindung lässt sich so von monovalentem Binden mehrerer Moleküle unterscheiden

Mechanism of multivalent carbohydrate-protein interactions studied by EPR spectroscopy

From a distance: Distance measurements in the nanometer range by means of spin-label electron paramagnetic resonance provide structural evidence for multivalent protein–ligand interactions in solution (see picture; protein subunits: blue/green, ligand: black, spin labels: yellow circles, binding sites: yellow letters). The data show a detailed picture of the binding of divalent ligands to a lectin

The oncogenic fusion protein RUNX1-CBFA2T1 supports proliferation and inhibits senescence in t(8;21)-positive leukaemic cells-6

<p><b>Copyright information:</b></p><p>Taken from "The oncogenic fusion protein RUNX1-CBFA2T1 supports proliferation and inhibits senescence in t(8;21)-positive leukaemic cells"</p><p>BMC Cancer 2004;4():44-44.</p><p>Published online 6 Aug 2004</p><p>PMCID:PMC512292.</p><p>Copyright © 2004 Martinez et al; licensee BioMed Central Ltd.</p>A was isolated on day 8 and analyzed by real-time RT-PCR. The columns and error bars represent the means and standard deviations of three independent experiments. B. Cell cycle distribution of siRNA-treated cells in the absence and presence of growth factors. Kasumi-1 cells were analyzed on day 8 by FACS analysis as described in Materials and Methods. C. Amount of S phase cells in dependence on the length of RUNX1-CBFA2T1 depletion. D. Amount of senescent cells dependence on the length of RUNX1-CBFA2T1 depletion. Growth factors are indicated below the graphs. The data shown in B, C and D were obtained from the same time course experiment. Cells were electroporated with the indicated siRNAs at days 0, 4, 8 and 12, and were analyzed on days 4, 8, 12 and 16

The oncogenic fusion protein RUNX1-CBFA2T1 supports proliferation and inhibits senescence in t(8;21)-positive leukaemic cells-3

<p><b>Copyright information:</b></p><p>Taken from "The oncogenic fusion protein RUNX1-CBFA2T1 supports proliferation and inhibits senescence in t(8;21)-positive leukaemic cells"</p><p>BMC Cancer 2004;4():44-44.</p><p>Published online 6 Aug 2004</p><p>PMCID:PMC512292.</p><p>Copyright © 2004 Martinez et al; licensee BioMed Central Ltd.</p>rmined at the indicated days using trypan blue counting. Arrows indicate electroporations. B. Graphical representation of cell doubling times. The columns represent the means of three independent experiments. Error bars indicate standard deviations. *, p < 0.05 according to Student's t-test. C. Graphical representation of the cell cycle phase distribution. Kasumi-1 cells were electroporated at days 0 and 4, and were examined at day 8 using FACS analysis. The columns and error bars represent the mean values and standard deviations of three independent experiments. †, p < 0.01 according to Student's t-test. C. RUNX1-CBFA2T1 suppression is associated with increased CDKN1B (p27) levels. After electroporation at days 0 and 4, total cell lysates were prepared at day 8 and analyzed using immunoblotting. After CDKN1B detection, the membrane was stripped and reprobed with an anti-tubulin antibody. The siRNAs are indicated on top. Arrows on the left mark RUNX1-CBFA2T1, RUNX1, CDKN1B (p27) and tubulin proteins. The relative ratios between CDKN1B and tubulin are indicated below the blot

The oncogenic fusion protein RUNX1-CBFA2T1 supports proliferation and inhibits senescence in t(8;21)-positive leukaemic cells-0

<p><b>Copyright information:</b></p><p>Taken from "The oncogenic fusion protein RUNX1-CBFA2T1 supports proliferation and inhibits senescence in t(8;21)-positive leukaemic cells"</p><p>BMC Cancer 2004;4():44-44.</p><p>Published online 6 Aug 2004</p><p>PMCID:PMC512292.</p><p>Copyright © 2004 Martinez et al; licensee BioMed Central Ltd.</p> electroporation with 100 nM siRNAs and analyzed by immunoblotting. The electroporated cells and siRNAs are indicated on top. Arrows on the right mark RUNX1-CBFA2T1 and RUNX1 proteins. Markers are shown on the left, and the relative ratios between RUNX1-CBFA2T1 and RUNX1 are indicated below the blot. B. Time course of siRNA-mediated RUNX1-CBFA2T1 depletion. Kasumi-1 nuclear lysates were isolated at the indicated days after electroporation with 100 nM siRNA and analyzed by immunoblotting. Values were normalized to the control siRNA siAGF6