Modeling and control of the acrylic acid synthesis by fermentative process

Orientador: Rubens Maciel FilhoTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia QuimicaResumo: A síntese de ácido acrílico via processo fermentativo é um assunto recente, com poucas informações disponíveis na literatura e, tem despertado cada vez mais interesse de pesquisadores. Através da modelagem matemática, é possível obter uma melhor compreensão de um processo em sua totalidade, através de analogias abstratas que possibilitam a predição de um fenômeno. O objetivo deste trabalho consiste no desenvolvimento de modelos determinísticos para representar o processo biotecnológico de síntese de ácido acrílico a partir de uma rota metabólica alternativa, visando estudos do comportamento estacionário/dinâmico do processo, aplicação de técnicas de planejamento experimental para identificação dos parâmetros mais relevantes e otimização dos parâmetros operacionais, com a finalidade de compreender o processo e apresentar uma metodologia alternativa para a sua produção. A partir dos modelos desenvolvidos foi possível obter perfis de concentração dos principais componentes do processo. Depois de identificadas as influências dos parâmetros operacionais, cinéticos e de projeto sobre o processo, os parâmetros operacionais de cada modelo foram otimizados através da aplicação da metodologia de superfície de resposta e do método de otimização por programação quadrática sucessiva. Com a otimização dos parâmetros foi possível encontrar condições de operação que aumentaram a concentração e o rendimento final de ácido acrílico, como também, perceber as limitações dos métodos de otimização usados. Nos modelos com cinética estruturada foram aplicadas estratégias de controle, através das quais foi possível encontrar condições ótimas, podendo com isso operar o biorreator de forma eficiente e segura, além de aumentar o rendimento final de ácido acrílico. Através da realização deste trabalho, pode-se concluir que os modelos desenvolvidos constituem um conjunto de ferramentas interessantes para predizer e investigar o comportamento do processo de síntese de ácido acrílico via processo fermentativo, uma vez que, a literatura ainda não dispõe de trabalhos de modelagem para a síntese de ácido acrílico via rotas fermentativas. Também, servir de apoio para estudos de manipulação genética visando obter microrganismos com capacidade de produzir ácido acrílico em condições economicamente competitivasAbstract: The acrylic acid synthesis by fermentative process is a recent subject, with few available information in literature and it has demonstrated each time more interest of researchers. Through the mathematical modeling, it is possible to obtain a better understanding of the process behavior if mechanistic models are developed. The purpose of this work is the deterministic model development for the biotechnological process of acrylic acid synthesis through an alternative metabolic route, aiming the studies of both the steady state and dynamic behavior of the process. To investigate the parameters interactions as well as to identify the parameters with the most significant impact on the model experimental design was used and operational parameters were optimized. The purpose is to understand the process and to present an alternative methodology for its production. From of the developed models was possible to obtain the concentration profiles of the process components main. After identified the influence of the operational, kinetic and design parameters about the process, the operational parameters were optimized through the application of the response surface methodology and the successive quadratic programming optimization method. With the optimization of parameters was possible to find out the optimal operational conditions and thus to increase the yield and acrylic acid concentration as well as to perceive the limitation of optimization methods used. In the models with structured kinetic were applied control strategies, where was possible to operate the bioreactor safe and efficiently as well as to increase the acrylic acid final yield. With the realization of this work, it follows that the models developed consist of a set of interesting tools to predict and investigate the behavior of the acrylic acid synthesis by fermentative process, whereas in the literature are not yet available works about modeling of the acrylic acid synthesis from fermentative routes, as well as can to serve as support for studies of genetic manipulation aiming at to obtain microorganisms with capacity to produce acrylic acid in competitive economically conditionsDoutoradoDesenvolvimento de Processos QuímicosDoutor em Engenharia Químic

A simple biorefinery concept to produce 2g-lactic acid from Sugar Beet Pulp (SBP): A high-value target approach to valorize awaste stream

Lactic acid is a high-value molecule with a vast number of applications. Its production in the biorefineries model is a possibility for this sector to aggregate value to its production chain. Thus, this investigation presents a biorefinery model based on the traditional sugar beet industry proposing an approach to produce lactic acid from a waste stream. Sugar beet is used to produce sugar and ethanol, and the remaining pulp is sent to animal feed. Using Bacillus coagulans in a continuous fermentation, 2781.01 g of lactic acid was produced from 3916.91 g of sugars from hydrolyzed sugar beet pulp, with a maximum productivity of 18.06 g L-1h-1. Without interfering in the sugar production, ethanol, or lactic acid, it is also possible to produce pectin and phenolic compounds in the biorefinery. The lactic acid produced was purified by a bipolar membrane electrodialysis and the recovery reached 788.80 g/L with 98% w/w purity. © 2020 by the authors

A Simple Biorefinery Concept to Produce 2G-Lactic Acid from Sugar Beet Pulp (SBP): A High-Value Target Approach to Valorize a Waste Stream

Lactic acid is a high-value molecule with a vast number of applications. Its production in the biorefineries model is a possibility for this sector to aggregate value to its production chain. Thus, this investigation presents a biorefinery model based on the traditional sugar beet industry proposing an approach to produce lactic acid from a waste stream. Sugar beet is used to produce sugar and ethanol, and the remaining pulp is sent to animal feed. Using Bacillus coagulans in a continuous fermentation, 2781.01 g of lactic acid was produced from 3916.91 g of sugars from hydrolyzed sugar beet pulp, with a maximum productivity of 18.06 g L−1h−1. Without interfering in the sugar production, ethanol, or lactic acid, it is also possible to produce pectin and phenolic compounds in the biorefinery. The lactic acid produced was purified by a bipolar membrane electrodialysis and the recovery reached 788.80 g/L with 98% w/w purity