Etude épidémiologique et cliniques des pleuropneumopathies infectieuses de l'enfant au centre hospitalier et universitaire de Reims de 1996 à 2006 (Actualisation de leur prise en charge)

REIMS-BU Santé (514542104) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

Devenir respiratoire à l'âge adulte de nourrissons ayant présenté une première bronchiolite avec hospitalisation avant l'âge de 24 mois

REIMS-BU Santé (514542104) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

Etude épidemiologique et clinique des infections respiratoires à mycoplasma pneumoniae de l'enfant au centre hospitalier et universitaire de Reims de janvier 1999 à juillet 2005

REIMS-BU Santé (514542104) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

Epidémiologie et impact du Staphylococcus aureus résistant à la méticilline dans la cohorte pédiatrique du CRCM de Reims

REIMS-BU Santé (514542104) / SudocSudocFranceF

Corps étrangers respiratoires de l'enfant

REIMS-BU Santé (514542104) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

Early Onset of Sleep-Disordered Breathing in Two Children With SEPN1 -Related Myopathies

International audienceSelenoprotein-related myopathy (SEPN1-RM) is a rare disease with a variable clinical presentation. The selenoprotein N1 gene (SEPN1) mutation causing this congenital muscular dystrophy was identified in 2001. Sleep-disordered breathing (SDB) may occur in young SEPN1-RM patients who are still able to walk. We report here the cases of two children with SEPN1-RM who presented with SDB at the ages of 7 and 12 years and for whom long-term nocturnal noninvasive ventilation yielded significant improvement. Based upon literature review and our current cases, it seems that there is no obvious relationship between time since SDB onset and outcome of pulmonary function tests or limb muscle weakness. We therefore suggest that sleep-disordered breathing should systematically be screened for in patients with SEPN1-RM, at regular intervals using nocturnal polysomnography

42 Nasal epithelial brushing : a valuable method to study airway epithelial cells in CF and non-CF infants

To better understand the early stages of cystic fribrosis disease (CF), it is of major interest to study respiratory epithelial cells obtained as early as possible in humans. Nasal epithelial brushing, a much less invasive technique than broncho-alveolar purpose of the present study was to assess its feassibility in CF infants

Feasibility of nasal epithelial brushing for the study of airway epithelial functions in CF infants.

International audienceBACKGROUND: For a better understanding of the early stages of cystic fibrosis (CF), it is of major interest to study respiratory epithelial cells obtained as early as possible. Although bronchoalveolar lavage has been proposed for this purpose, nasal brushing, which is a much less invasive technique, has seldom been used in CF infants. The aim of the present study was to examine in a few infants the feasibility of a nasal brushing technique for studies of airway epithelial functions in very young CF infants. METHODS: In 5 CF (median age 12, range 1-18 months) and 10 control infants (median age 5, range 1-17 months), a nasal brushing was performed by means of a soft sterile cytology brush, after premedication with oral paracetamol (15 mg/kg body weight) and rectal midazolam (0.2 mg/kg body weight). Samples were used for microbiological, cytological and functional studies. RESULTS: The procedure was well tolerated. Number of cells collected was similar in CF and non-CF patients (CF: median 230x10(3), range 42x10(3)-900x10(3); non-CF: median 340x10(3), range 140x10(3)-900x10(3)). Median number of viable cells was 67% (range 31-84%). Freshly obtained samples were successfully used for studies of ciliary beating frequency and cAMP-dependent chloride efflux. In 7 out of 17 cell cultures, confluence was obtained (CF: 2 out of 7; non-CF: 5 out of 10). The feasibility of studying protein release and mRNA expression of IL-8, IL-6 and TNF-alpha, under basal conditions and after stimulation by Pseudomonas aeruginosa, was demonstrated. CONCLUSIONS: By means of a simple nasal brushing technique easily performed and well tolerated, it is feasible, in infants, to harvest respiratory cells in sufficient amounts to study the airway epithelium using a broad range of techniques including cell culture

Real-world assessment of LCI following lumacaftor-ivacaftor initiation in adolescents and adults with cystic fibrosis

International audienc