Freedom of Choice as Control over Outcomes

As interactions between individuals are introduced into the freedom of choice literature by the mean of game forms, new issues appear. In particular, in this paper it is argued that individuals face uncertainty with respect to outcomes as they lose the control they implicitely exert over options in the opportunity set framework. A criterion is proposed as to compare alternative game forms in terms of the control they offer to individuals. The CardMin criterion suggests that any game form should be judged on the basis of the strategy offering the lowest number of pairwise different outcomes. An axiomatic characterization is provided in the case of two individuals.Freedom of Choice, Control, Axiomatic, Game Forms

Appraising Diversity with an Ordinal Notion of Similarity: An Axiomatic Approach

This paper provides an axiomatic characterization of two rules for comparing alternative sets of objects on the basis of the diversity that they offer. The framework considered assumes a finite universe of objects and an a priori given ordinal quadernary relation that compares alternative pairs of objects on the basis of their ordinal dissimilarity. Very few properties of this quadernary relation are assumed (beside completeness, transitivity and a very natural form of symmetry). The two rules that we characterize are the maxi-max criterion and the lexi-max criterion. The maxi-max criterion considers that a set is more diverse than another if and only if the two objects that are the most dissimilar in the former are weakly as dissimilar as the two most dissimilar objects in the later. The lexi-max criterion is defined as usual as the lexicographic extension of the maxi-max criterion. Some connections with the broader issue of measuring freedom of choice are also provided.Diversity, Measurement, Axioms, Freedom of choice

Use of sigma factor M from Bacillus subtilis in the development of an orthogonal expression system in Escherichia coli

Background: Technological advances in synthetic biology, systems biology, and metabolic engineering have boosted applications of industrial biotechnology for an increasing number of complex and high added-value molecules. In general, the transfer of multi- gene or poorly understood heterologous pathways into the production host leads to imbalances due to lack of adequate regulatory mechanisms. Hence, fine-tuning expression of synthesis pathways in specific conditions is mandatory. Objectives: Here we develop a new genetic circuit for regulated expression specifically in stationary phase due to clear advantages during this period (reduction of toxicity, competition). Methods: This circuit consists of a heterologous sigma factor () recognizing specific promoter sequences, which are not recognised by the native factors of E. coli and is expressed upon entering the stationary phase. First, several factors of B. subtilis were tested for their orthogonality in E. coli on the level of promoter recognition, by using a red-fluorescent reporter system. Secondly, the potential of factors of B. subtilis to work together with the E. coli core RNA polymerase was tested, by expressing these proteins together with their promoters. Based on the results a specific factor will be chosen for further optimalisation and the corresponding gene can be cloned in the S factor operon of E. coli, which is most abundantly expressed in stationary conditions. Conclusions: Combining all these elements should allow us to create an orthogonal genetic circuit that is able to transcribe specific genes under stationary phase with a limited influence on the host cell’s metabolism

A Renewed Analysis of Cheating in Contests: Theory and Evidence from Recovery Doping

In rank-order tournaments, players have incentives to cheat in order to increase their probability of winning the prize. Usually, cheating is seen as a technology that allows individuals to illegally increase their best potential performances. This paper argues that cheating can alternatively be seen as a technology that ensures that the best performances are reached more often. We call this technology recovery doping and show that it yields new insights on the effects of cheating: recovery doping lowers performance uncertainty, thereby changing the outcome of the contest in favour of the best players. We develop this theory in a game with player heterogeneity and performance uncertainty and then study the results of the cross-country skiing World Cup between 1987 and 2006. In line with our theoretical predictions, race-specific rankings were remarkably stable during the 1990s, subsequently becoming more volatile. This pattern reflects the rise and fall of synthetic EPO and the emergence of blood testing and profiling

Phenomenology for Introductory Architectural Analysis Courses: The pentagon methodological approach

As a consequence of fruitful discussions about joining theory and practice both in design research and educational design programmes, this article aims to explore phenomenological parameters in the framework of an exercise for Engineer-architecture students from the University of Leuven in Belgium. Relying upon the arguments of recognised architects regarding the importance of the phenomenological approach in the field of architecture, it is intended to propose a five-step method (pentagon) to add to architectural analytical exercises. The paper argues that an explicit phenomenological awareness within architectural design education should be addressed in addition to the potential references to architectural phenomenology in theoretical courses or in the discourse of architectural design teachers during the studio courses. This article begins this process through the discussion of one example: ‘Integrated Seminar on Housing’ which is taught in the first semester of the bachelor programme. A qualitative review of theoutcomes of the exercise stresses a positive effect in the development of students’ skills that are not an explicit focus of methodologies related to programmatic or technical skills. The conclusions encourage the development of the experimental study to improve the complementarity of the phenomenological approach with the more technical methodologies. In the final reflectionsabout the results of the pentagon methodological approach some evidence is provided in respect to the article’s claims

A sigma factor toolbox for orthogonal gene expression in Escherichia coli

Synthetic genetic sensors and circuits enable programmable control over timing and conditions of gene expression and, as a result, are increasingly incorporated into the control of complex and multi-gene pathways. Size and complexity of genetic circuits are growing, but stay limited by a shortage of regulatory parts that can be used without interference. Therefore, orthogonal expression and regulation systems are needed to minimize undesired crosstalk and allow for dynamic control of separate modules. This work presents a set of orthogonal expression systems for use in Escherichia coli based on heterologous sigma factors from Bacillus subtilis that recognize specific promoter sequences. Up to four of the analyzed sigma factors can be combined to function orthogonally between each other and toward the host. Additionally, the toolbox is expanded by creating promoter libraries for three sigma factors without loss of their orthogonal nature. As this set covers a wide range of transcription initiation frequencies, it enables tuning of multiple outputs of the circuit in response to different sensory signals in an orthogonal manner. This sigma factor toolbox constitutes an interesting expansion of the synthetic biology toolbox and may contribute to the assembly of more complex synthetic genetic systems in the future