La perdiu roja, reservori de la grip aviar d'alta patogenicitat

La influença aviària ha esdevingut una de les malalties animals més importants dins la sanitat animal i la salut pública, però encara conserva moltes preguntes per respondre al seu voltant. Com aquesta malaltia afecta les espècies aviàries més conegudes, com ànecs i gallines, ha estat àmpliament estudiat, però encara calen estudis de les espècies de producció menor com les perdius, els faisans i les guatlles, que poden actuar de reservori de la malaltia. Investigadors de la UAB han estudiat l'efecte de la infecció experimental en perdius roges amb dos tipus de virus de la grip aviar, altament patògens i de patogenicitat baixa, i han determinat que aquest animal és un reservori important del primer tipus, però no del segon.La influenza aviar, ha convertido en una de las enfermedades animales más importantes dentro de la sanidad animal y la salud pública, pero aún conserva muchas preguntas por responder a su alrededor. Como esta enfermedad afecta a las especies aviares más conocidas, como patos y gallinas, ha sido ampliamente estudiado, pero aún se necesitan estudios de las especies de producción menor como las perdices, los faisanes y las codornices, que pueden actuar de reservorio de la enfermedad. Investigadores de la UAB han estudiado el efecto de la infección experimental en perdices rojas con dos tipos de virus de la gripe aviar, altamente patógenos y de patogenicidad baja, y han determinado que este animal es un reservorio importante del primer tipo, pero no del segundo

The use of H-index to assess research priorities in poultry diseases

Identifying which diseases represent a priority is crucial to optimize resources for diagnostics, control, and prevention. Here, the impact of 111 poultry pathogens belonging to Viruses (n = 31), Bacteria (n = 33), and Other (n = 47) was assessed using the H-index. The overall mean H-indexes suggested that poultry Viruses have statistically greater impact than Bacteria, which in turn are statistically more relevant than Others. Among the 20 highest H-indexes, 45% were zoonotic, and almost a third was Office International des Epizooties-listed. Avian influenza virus (H-index 127), Salmonella enteritidis and Salmonella typhimurium (H-index 72), and Eimeria spp (H-index 70) ranked the highest in Virus, Bacteria, and Other, respectively. Pathogens that produce overt clinical diseases and economic damage, cause immunosuppression, and/or are zoonotic had the highest H-index scores. The evolution of citations of particular pathogens reflected severe poultry outbreaks and/or zoonotic outbreaks in relatively wide geographic areas. Also, the evolution of citations based on taxonomic groups mirrored major changes in poultry production practices and management throughout history. Thus, Others were the most cited pathogens until the 1970s and, following 3 decades of unpopularity because of widespread use of intensive production practices, regained importance in the 2000s thanks to welfare regulation changes. Citations for Bacteria increased especially from the 1990s onward, probably because of the ban of growth promoters in western countries and the need to find new control methods for bacterial and protozoal infections. In general, countries with the greatest poultry production and research budgets had higher research production, that is the United States of America (USA) and China. Interestingly, the United Kingdom was among the top research producers despite falling behind other countries in poultry production and research budget. Moreover, the USA exhibited the strongest poultry research production based on number and diversity of publications (Dcos-index). In conclusion, the H-index could be a valid, simple tool to prioritize funding or interest in poultry diseases, especially when used as a preliminary selection approach in combination with other metrics

Special Issue "Viral Shedding and Transmission in Zoonotic Diseases"

The papers published in this Special Issue represent only a glimpse of the vast diversity of viral infectious diseases, and the complexity of their interactions with the host, that have an impact on human and animal health [...

La influença aviària en aus domèstics i salvatges

Una tesi doctoral llegida al CReSA a la UAB ha estudiat el virus de la influença aviària, o grip aviària, en tres espècies d'aus autòctones que tant poden ser salvatges com domèstiques: la perdiu roja, la guatlla europea i el falcó sacre-grifó. Els resultats d'aquest treball mostren que totes tres espècies podrien representar papers importants en el cas que es produïssin brots de diferents tipus de soques. Així, el coneixement de la dinàmica de la infecció i la transmissió en aquestes aus és crucial per a una millor vigilància i gestió dels brots d'aquest virus a Catalunya.Una tesis doctoral leída en el CReSA en la UAB ha estudiado el virus de la influenza aviar, o gripe aviar, en tres especies de aves autóctonas que se encuentran en el término medio entre aves salvajes y domésticas: la perdiz roja, la codorniz europea y el halcón gerifalte-sacre. Los resultados de este trabajo muestran que las tres especies podrían representar papeles importantes en el caso de que se dieran brotes de diferentes tipos de cepas. Así, el conocimiento de la dinámica de la infección en estas aves es crucial para una mejor vigilancia y gestión de los brotes de este virus en Cataluña

Pathobiology and innate immune responses of gallinaceous poultry to clade 2.3.4.4A H5Nx highly pathogenic avian influenza virus infection

In the 2014-2015 Eurasian lineage clade 2.3.4.4A H5 highly pathogenic avian influenza (HPAI) outbreak in the U.S., backyard flocks with minor gallinaceous poultry and large commercial poultry (chickens and turkeys) operations were affected. The pathogenesis of the first H5N8 and reassortant H5N2 clade 2.3.4.4A HPAI U.S. isolates was investigated in six gallinaceous species: chickens, Japanese quail, Bobwhite quail, Pearl guinea fowl, Chukar partridges, and Ring-necked pheasants. Both viruses caused 80-100% mortality in all species, except for H5N2 virus that caused 60% mortality in chickens. The surviving challenged birds remained uninfected based on lack of clinical disease and lack of seroconversion. Among the infected birds, chickens and Japanese quail in early clinical stages (asymptomatic and listless) lacked histopathologic findings. In contrast, birds of all species in later clinical stages (moribund and dead) had histopathologic lesions and systemic virus replication consistent with HPAI virus infection in gallinaceous poultry. These birds had widespread multifocal areas of necrosis, sometimes with heterophilic or lymphoplasmacytic inflammatory infiltrate, and viral antigen in parenchymal cells of most tissues. In general, lesions and antigen distribution were similar regardless of virus and species. However, endotheliotropism was the most striking difference among species, with only Pearl guinea fowl showing widespread replication of both viruses in endothelial cells of most tissues. The expression of IFN-γand IL-10 in Japanese quail, and IL-6 in chickens, were up-regulated in later clinical stages compared to asymptomatic birds

A 10-Year Retrospective Study of Inclusion Body Hepatitis in Meat-Type Chickens in Spain (2011–2021)

A surge in fowl adenovirus (FAdV) causing inclusion body hepatitis (IBH) outbreaks has occurred in several countries in the last two decades. In Spain, a sharp increase in case numbers in broilers and broiler breeder pullets arose since 2011, which prompted the vaccination of breeders in some regions. Our retrospective study of IBH cases in Spain from 2011 to 2021 revealed that most cases were reported in broilers (92.21%) and were caused by serotypes FAdV-8b and -11, while cases in broiler breeder pullets were caused by serotypes FAdV-2, -11, and -8b. Vertical transmission was the main route of infection, although horizontal transmission likely happened in some broiler cases. Despite the inconsistent and heterogeneous use of vaccines among regions and over time, the number of cases mirrored the use of vaccines in the country. While IBH outbreaks were recorded year-long, significantly more cases occurred during the cooler and rainier months. The geographic distribution suggested a widespread incidence of IBH and revealed the importance of a highly integrated system. Our findings contribute to a better understanding of FAdV infection dynamics under field conditions and reiterate the importance of surveillance, serological monitoring of breeders, and vaccination of breeders against circulating serotypes to protect progenies.info:eu-repo/semantics/publishedVersio

Pathobiology and innate immune responses of gallinaceous poultry to clade 2.3.4.4A H5Nx highly pathogenic avian influenza virus infection

In the 2014–2015 Eurasian lineage clade 2.3.4.4A H5 highly pathogenic avian infuenza (HPAI) outbreak in the U.S., backyard focks with minor gallinaceous poultry and large commercial poultry (chickens and turkeys) operations were afected. The pathogenesis of the frst H5N8 and reassortant H5N2 clade 2.3.4.4A HPAI U.S. isolates was investi‑ gated in six gallinaceous species: chickens, Japanese quail, Bobwhite quail, Pearl guinea fowl, Chukar partridges, and Ring-necked pheasants. Both viruses caused 80–100% mortality in all species, except for H5N2 virus that caused 60% mortality in chickens. The surviving challenged birds remained uninfected based on lack of clinical disease and lack of seroconversion. Among the infected birds, chickens and Japanese quail in early clinical stages (asymptomatic and listless) lacked histopathologic fndings. In contrast, birds of all species in later clinical stages (moribund and dead) had histopathologic lesions and systemic virus replication consistent with HPAI virus infection in gallinaceous poultry. These birds had widespread multifocal areas of necrosis, sometimes with heterophilic or lymphoplasmacytic infam‑ matory infltrate, and viral antigen in parenchymal cells of most tissues. In general, lesions and antigen distribution were similar regardless of virus and species. However, endotheliotropism was the most striking diference among species, with only Pearl guinea fowl showing widespread replication of both viruses in endothelial cells of most tis‑ sues. The expression of IFN-γ and IL-10 in Japanese quail, and IL-6 in chickens, were up-regulated in later clinical stages compared to asymptomatic birds.info:eu-repo/semantics/publishedVersio

Pathobiology and innate immune responses of gallinaceous poultry to clade 2.3.4.4A H5Nx highly pathogenic avian influenza virus infection

International audienceAbstractIn the 2014–2015 Eurasian lineage clade 2.3.4.4A H5 highly pathogenic avian influenza (HPAI) outbreak in the U.S., backyard flocks with minor gallinaceous poultry and large commercial poultry (chickens and turkeys) operations were affected. The pathogenesis of the first H5N8 and reassortant H5N2 clade 2.3.4.4A HPAI U.S. isolates was investigated in six gallinaceous species: chickens, Japanese quail, Bobwhite quail, Pearl guinea fowl, Chukar partridges, and Ring-necked pheasants. Both viruses caused 80–100% mortality in all species, except for H5N2 virus that caused 60% mortality in chickens. The surviving challenged birds remained uninfected based on lack of clinical disease and lack of seroconversion. Among the infected birds, chickens and Japanese quail in early clinical stages (asymptomatic and listless) lacked histopathologic findings. In contrast, birds of all species in later clinical stages (moribund and dead) had histopathologic lesions and systemic virus replication consistent with HPAI virus infection in gallinaceous poultry. These birds had widespread multifocal areas of necrosis, sometimes with heterophilic or lymphoplasmacytic inflammatory infiltrate, and viral antigen in parenchymal cells of most tissues. In general, lesions and antigen distribution were similar regardless of virus and species. However, endotheliotropism was the most striking difference among species, with only Pearl guinea fowl showing widespread replication of both viruses in endothelial cells of most tissues. The expression of IFN-γ and IL-10 in Japanese quail, and IL-6 in chickens, were up-regulated in later clinical stages compared to asymptomatic birds

Efficacy of multivalent recombinant herpesvirus of turkey vaccines against high pathogenicity avian influenza, infectious bursal disease, and Newcastle disease viruses

Vaccines are an essential tool for the control of viral infections in domestic animals. We generated recombinant vector herpesvirus of turkeys (vHVT) vaccines expressing computationally optimized broadly reactive antigen (COBRA) H5 of avian influenza virus (AIV) alone (vHVT-AI) or in combination with virus protein 2 (VP2) of infectious bursal disease virus (IBDV) (vHVT-IBD-AI) or fusion (F) protein of Newcastle disease virus (NDV) (vHVT-ND-AI). In vaccinated chickens, all three vHVT vaccines provided 90–100% clinical protection against three divergent clades of high pathogenicity avian influenza viruses (HPAIVs), and significantly decreased number of birds and oral viral shedding titers at 2 days post-challenge compared to shams. Four weeks after vaccination, most vaccinated birds had H5 hemagglutination inhibition antibody titers, which significantly increased post-challenge. The vHVT-IBD-AI and vHVT-ND-AI vaccines provided 100% clinical protection against IBDVs and NDV, respectively. Our findings demonstrate that multivalent HVT vector vaccines were efficacious for simultaneous control of HPAIV and other viral infections.info:eu-repo/semantics/publishedVersio