Green tea glycolic extract-loaded liquid crystal systems: development, characterization and microbiological control

Liquid crystal systems (LCSs) have interesting cosmetic applications because of their ability to increase the therapeutic efficiency and solubility of active ingredients. The aim of the present research was to develop green tea glycolic extract-loaded LCSs, to characterize and to perform microbiological control. The ternary phase diagram was constructed using polysorbate 20, silicone glycol copolymer (SGC) - DC 193(r), and distilled water with 1.5% glycolic green tea extract. The systems were characterized by polarized light microscopy. Formulations selected were characterized as transparent viscous systems and transparent liquid system indicated mesophases lamellar structure. The results of the microbiological analysis of mesophilic aerobic microorganisms (bacteria and fungi) revealed that the above formulation showed a biologic loa

Development of nanotechnology-based drug delivery systems with olive vegetable oil for cutaneous application

Liquid-Crystalline Systems represent active compounds delivery systems that may be able to overcome the physical barrier of the skin, especially represented by the stratum corneum. To obtain these systems, aqueous and oily components are used with surfactants. Of the different association structures in such systems, the liquid-crystalline offer numerous advantages to a topical product. This manuscript presents the development of liquid-crystalline systems consisting, in which the oil component is olive oil, its rheological characterizations, and the location of liquid crystals in its phase map. Cytotoxic effects were evaluated using J-774 mouse macrophages as the cellular model. A phase diagram to mix three components with different proportions was constructed. Two liquid crystalline areas were found with olive oil in different regions in the ternary diagram with two nonionic surfactants, called SLC1 (S1) and SLC2 (S2). These systems showed lamellar liquid crystals that remained stable during the entire analysis time. The systems were also characterized rheologically with pseudoplastic behavior without thixotropy. The texture and bioadhesion assays showed that formulations were similar statistically (p < 0.05), indicating that the increased amount of water in S2 did not interfere with the bioadhesive properties of the systems. In vitro cytotoxic assays showed that formulations did not present cytotoxicity. Olive oil-based systems may be a promising platform for skin delivery of drugs.Os cristais líquidos representam um sistema de liberação de substâncias ativas capazes de vencer a barreira cutânea, representada especialmente pelo estrato córneo. Água, óleo e tensoativos são misturados para se obter esses sistemas. Diferentes estruturas podem ser formadas nesses sistemas, as quais oferecem muitas vantagens para os produtos de uso tópico. Esse trabalho visou ao desenvolvimento de sistemas líquido-cristalinos preparados com óleo de oliva, sua caracterização reológica e a identificação das fases cristalinas no diagrama ternário. Efeitos citotóxicos foram avaliados usando células de rato como modelo celular. Construiu-se um diagrama de fases que mistura três componentes em diferentes proporções. Duas áreas de cristal líquido, denominadas SLC1 (S1) e SLC2 (S2), foram encontradas com óleo de oliva em diferentes regiões no diagrama ternário preparado com dois diferentes tensoativos não-iônicos. Esses sistemas mostraram fase cristalina lamelar, que permaneceu estável durante o tempo estudado. Os sistemas foram também caracterizados reologicamente e apresentaram comportamento pseudoplástico com tixotropia. Os ensaios de textura e bioadesão mostraram que as formulações foram similares (p < 0.05), indicando que o aumento da quantidade de água em S2 não interferiu nas propriedades bioadesivas dos sistemas. Os ensaios de citotoxicidade mostraram que as formulações não foram citotóxicas. Sistemas à base de óleo de oliva são interessantes para a liberação de fármacos na pele

Desenvolvimento, caracterização e ação anti-biofilme oralde sistemas nanoestruturados bioadesivos contendo o peptídeo KSL-W

Periodontal diseases affect a large part of the population, being one of the causes of tooth loss in humans. Their chronic manifestations can be divided briefly in gingivitis and periodontitis, depending on the existence or not of teeth support tissue involvement. It is known that its etiology involves, among other factors, the accumulation of dental biofilm supra- and subgingival. Thus, studies seek to assess the antimicrobial potential of substances that have action against multispecies biofilms. Recently, a growing interest it has been observed in the study of peptides that demonstrate antimicrobial action against microorganisms present in the dental biofilm, as the KSL-W peptide. Nanostructured systems of drug release, as the liquidcrystalline systems (LCS), have long been used because of increasing stability and the efficacy of drugs, beyond modulate their action, that make them suitable to be used like peptide carrier. The potentialization of delivery system can, possibly, be achieved with the presence of mucoadesivas substances, essential for the prolonged stay of the formulation in the action place. Thereby, it is intended to develop and characterize nanostructured systems mucoadesivos, as the LCS, for incorporation of the synthetic peptide KSL-W, and later, evaluate the in vitro antimicrobial action in multispecie biofilms grown on the surface dental root. For that, developed liquid-crystalline systems constituted by cetyl alcohol ethoxylate and propoxilado - Procetyl® AWS (surfactant, S), oleic acid (oily phase, OP) and dispersion of poloxamer 407 at concentrations of 0.5%, 0.75% e 1% (the aqueous phase, AP), obtaining the diagrams S1, S2 e S3. It was selected 4 points of each diagram keeping the concentration of surfactant in 50% and varying the proportions of the AP and OP, F27 - 50% S, 10% OP and 40% AP; F28 - 50% S, 20% OP and 30% AP; F29 - 50% S, 30% OP and 20% AP; F30 - 50% S, 40% OP and 10% AP. Posteriorly, the ...As doenças periodontais acometem grande parte da população, sendo uma das causas de perdas dentárias em humanos. As suas manifestações clínicas podem ser divididas genericamente em gengivite e periodontite, dependendo da existência ou não de envolvimento do tecido de sustentação dos dentes. Sabe-se que sua etiologia envolve, dentre outros fatores, o acúmulo de biofilme dental supra- e subgengival. Dessa maneira, estudos buscam avaliar o potencial antimicrobiano de substâncias que possuam ação contra biofilme dental multiespécie. Recentemente, um crescente interesse tem sido observado no estudo de peptídeos, como o KSL-W, que demonstram ação antimicrobiana contra micro-organismos presentes no biofilme dental. Sistemas nanoestruturados de liberação de fármacos, como os sistemas líquido-cristalinos (SLC), têm sido muito utilizados pelo fato de aumentarem a estabilidade e a eficácia de fármacos, além de modularem sua ação, o que os torna aptos a serem usados como carreadores de peptídeos. A potencialização destes sistemas de liberação pode, possivelmente, ser conseguida com a presença de substâncias mucoadesivas, essenciais para a permanência prolongada da formulação no local de ação. Portanto, o objetivo deste trabalho foi desenvolver e caracterizar sistemas nanoestruturados mucoadesivos, como os SLC, para incorporação do peptídeo sintético KSL-W, e posteriormente, avaliar a ação in vitro em biofilme multiespécie salivar. Para isso, foram desenvolvidos sistemas líquidocristalinos constituídos por álcool cetílico propoxilado e etoxilado - Procetyl® AWS (tensoativo, T), ácido oleico (fase oleosa, FO) e dispersão de poloxamer 407 nas concentrações de 0,5%, 0,75% e 1% (fase aquosa, FA), obtendo-se os diagramas S1, S2 e S3, respectivamente. Selecionou-se 4 pontos de cada um dos diagramas mantendo-se a concentração do T em 50% e variando-se as proporções de FA e FO, a saber F27 - 50% T, 10% FO...Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Nanotechnology-Based Drug Delivery Systems for Photodynamic Therapy of Cancer: A Review

Photodynamic therapy (PDT) is a promising alternative approach for improved cancer treatment. In PDT, a photosensitizer (PS) is administered that can be activated by light of a specific wavelength, which causes selective damage to the tumor and its surrounding vasculature. The success of PDT is limited by the difficulty in administering photosensitizers (PSs) with low water solubility, which compromises the clinical use of several molecules. Incorporation of PSs in nanostructured drug delivery systems, such as polymeric nanoparticles (PNPs), solid lipid nanoparticles (SLNs), nanostructured lipid carriers (NLCs), gold nanoparticles (AuNPs), hydrogels, liposomes, liquid crystals, dendrimers, and cyclodextrin is a potential strategy to overcome this difficulty. Additionally, nanotechnology-based drug delivery systems may improve the transcytosis of a PS across epithelial and endothelial barriers and afford the simultaneous co-delivery of two or more drugs. Based on this, the application of nanotechnology in medicine may offer numerous exciting possibilities in cancer treatment and improve the efficacy of available therapeutics. Therefore, the aim of this paper is to review nanotechnology-based drug delivery systems for photodynamic therapy of cancer

Green tea glycolic extract-loaded liquid crystal systems: development, characterization and microbiological control

ABSTRACT Liquid crystal systems (LCSs) have interesting cosmetic applications because of their ability to increase the therapeutic efficiency and solubility of active ingredients. The aim of the present research was to develop green tea glycolic extract-loaded LCSs, to characterize and to perform microbiological control. The ternary phase diagram was constructed using polysorbate 20, silicone glycol copolymer (SGC) - DC 193(r), and distilled water with 1.5% glycolic green tea extract. The systems were characterized by polarized light microscopy. Formulations selected were characterized as transparent viscous systems and transparent liquid system indicated mesophases lamellar structure. The results of the microbiological analysis of mesophilic aerobic microorganisms (bacteria and fungi) revealed that the above formulation showed a biologic load <10 CFU/mL in all samples. In conclusion, liquid crystalline systems that have presented formation of a lamellar mesophases were developed. Furthermore, the formulation and products tested presented the adequate microbiological quality in accordance with official recommendations

Development of nanotechnology-based drug delivery systems with olive vegetable oil for cutaneous application

ABSTRACT Liquid-Crystalline Systems represent active compounds delivery systems that may be able to overcome the physical barrier of the skin, especially represented by the stratum corneum. To obtain these systems, aqueous and oily components are used with surfactants. Of the different association structures in such systems, the liquid-crystalline offer numerous advantages to a topical product. This manuscript presents the development of liquid-crystalline systems consisting, in which the oil component is olive oil, its rheological characterizations, and the location of liquid crystals in its phase map. Cytotoxic effects were evaluated using J-774 mouse macrophages as the cellular model. A phase diagram to mix three components with different proportions was constructed. Two liquid crystalline areas were found with olive oil in different regions in the ternary diagram with two nonionic surfactants, called SLC1 (S1) and SLC2 (S2). These systems showed lamellar liquid crystals that remained stable during the entire analysis time. The systems were also characterized rheologically with pseudoplastic behavior without thixotropy. The texture and bioadhesion assays showed that formulations were similar statistically (p < 0.05), indicating that the increased amount of water in S2 did not interfere with the bioadhesive properties of the systems. In vitro cytotoxic assays showed that formulations did not present cytotoxicity. Olive oil-based systems may be a promising platform for skin delivery of drugs

A curcumin-loaded liquid crystal precursor mucoadhesive system for the treatment of vaginal candidiasis

Women often develop vaginal infections that are caused primarily by organisms of the genus Candida. The current treatments of vaginal candidiasis usually involve azole-based antifungals, though fungal resistance to these compounds has become prevalent. Therefore, much attention has been given to molecules with antifungal properties from natural sources, such as curcumin (CUR). However, CUR has poor solubility in aqueous solvents and poor oral bioavailability. This study attempted to overcome this problem by developing, characterizing, and evaluating the in vitro antifungal action of a CUR-loaded liquid crystal precursor mucoadhesive system (LCPM) for vaginal administration. A low-viscosity LCPM (F) consisting of 40% wt/wt polyoxpropylene-(5)-polyoxyethylene-(20)-cetyl alcohol, 50% wt/wt oleic acid, and 10% wt/wt chitosan dispersion at 0.5% with the addition of 16% poloxamer 407 was developed to take advantage of the lyotropic phase behavior of this formulation. Notably, F could transform into liquid crystal systems when diluted with artificial vaginal mucus at ratios of 1:3 and 1:1 (wt/wt), resulting in the formation of F30 and F100, respectively. Polarized light microscopy and rheological studies revealed that F behaved like an isotropic formulation, whereas F30 and F100 behaved like an anisotropic liquid crystalline system (LCS). Moreover, F30 and F100 presented higher mucoadhesion to porcine vaginal mucosa than F. The analysis of the in vitro activity against Candida albicans revealed that CUR-loaded F was more potent against standard and clinical strains compared with a CUR solution. Therefore, the vaginal administration of CUR-loaded LCPMs represents a promising platform for the treatment of vaginal candidiasis