Using Facial EMG to Track Emotion During Language Comprehension: Past, Present, and Future

Beyond recognizing words, parsing sentences, building situation models, and other cognitive accomplishments, language comprehension always involves some degree of emotion too, with or without awareness. Language excites, bores, or otherwise moves us, and studying how it does so is crucial. This chapter examines the potential of facial electromyography (EMG) to study language-elicited emotion. After discussing the limitations of self-report measures, we examine various other tools to tap into emotion, and then zoom in on the electrophysiological recording of facial muscle activity. Surveying psycholinguistics, communication science, and other fields, we provide an exhaustive qualitative review of the relevant facial EMG research to date, exploring 55 affective comprehension experiments with single words, phrases, sentences, or larger pieces of discourse. We discuss the outcomes of this research, and evaluate the various practices, biases, and omissions in the field. We also present the fALC model, a new conceptual model that lays out the various potential sources of facial EMG activity during language comprehension. Our review suggests that facial EMG recording is a powerful tool for exploring the conscious as well as unconscious aspects of affective language comprehension. However, we also think it is time to take on a bit more complexity in this research field, by for example considering the possibility that multiple active generators can simultaneously contribute to an emotional facial expression, by studying how the communicator’s stance and social intention can give rise to emotion, and by studying facial expressions not just as indexes of inner states, but also as social tools that enrich everyday verbal interactions

The ab-anisotrophy of twinfree YBa₂Cu₃O_7-δ films above and below T_c

High quality twin-free c-axis oriented YBa2Cu3O7 films were grown by pulsed laser deposition on NdGaO3. We find resistive anisotropies between 1 < a/b < 1.6. The resistive anisotropy above Tc appears to be correlated to the anisotropy in the critical current below Tc. The normal state properties of the chains along b seem to have a significant influence on the superconducting properties of YBa2Cu3O7

When the Truth Is Not Too Hard to Handle: An Event-Related Potential Study on the Pragmatics of Negation

Our brains rapidly map incoming language onto what we hold to be true. Yet there are claims that such integration and verification processes are delayed in sentences containing negation words like not. However, studies have often confounded whether a statement is true and whether it is a natural thing to say during normal communication. In an event-related potential (ERP) experiment, we aimed to disentangle effects of truth value and pragmatic licensing on the comprehension of affirmative and negated real-world statements. As in affirmative sentences, false words elicited a larger N400 ERP than did true words in pragmatically licensed negated sentences (e.g., “In moderation, drinking red wine isn't bad/good…”), whereas true and false words elicited similar responses in unlicensed negated sentences (e.g., “A baby bunny's fur isn't very hard/soft…”). These results suggest that negation poses no principled obstacle for readers to immediately relate incoming words to what they hold to be true

Phonological facilitation of grammatical gender retrieval.

In Dutch, the gender of nouns is marked by the definite articles de (common gender) and het (neuter gender). Most models of language production assume that gender information is retrieved via the noun's syntactic representation (or lemma). The authors test Caramazza's (1997) alternative proposal, according to which gender information is retrieved via the noun's phonological word form (or lexeme). In three picture-word experiments, which differed in the tasks to be performed (noun production, article+noun production, article production, and gender decision), clear phonological effects were obtained in tasks involving the retrieval of the noun's gender information. It is argued that traditional models of language production have difficulty in accounting for the occurrence and/or the size of these effects whereas they follow quite naturally from Caramazza's (1997) Independent Network model. © 2004 Psychology Press Ltd

Spatial partitioning of the regulatory landscape of the X-inactivation centre

In eukaryotes transcriptional regulation often involves multiple long-range elements and is influenced by the genomic environment. A prime example of this concerns the mouse X-inactivation centre (Xic), which orchestrates the initiation of X-chromosome inactivation (XCI) by controlling the expression of the non-protein-coding Xist transcript. The extent of Xic sequences required for the proper regulation of Xist remains unknown. Here we use chromosome conformation capture carbon-copy (5C) and super-resolution microscopy to analyse the spatial organization of a 4.5-megabases (Mb) region including Xist. We discover a series of discrete 200-kilobase to 1 Mb topologically associating domains (TADs), present both before and after cell differentiation and on the active and inactive X. TADs align with, but do not rely on, several domain-wide features of the epigenome, such as H3K27me3 or H3K9me2 blocks and lamina-associated domains. TADs also align with coordinately regulated gene clusters. Disruption of a TAD boundary causes ectopic chromosomal contacts and long-range transcriptional misregulation. The Xist/Tsix sense/antisense unit illustrates how TADs enable the spatial segregation of oppositely regulated chromosomal neighbourhoods, with the respective promoters of Xist and Tsix lying in adjacent TADs, each containing their known positive regulators. We identify a novel distal regulatory region of Tsix within its TAD, which produces a long intervening RNA, Linx. In addition to uncovering a new principle of cis-regulatory architecture of mammalian chromosomes, our study sets the stage for the full genetic dissection of the X-inactivation centre

The Genome of the Acid Soil-Adapted Strain Rhizobium favelukesii OR191 Encodes Determinants for Effective Symbiotic Interaction With Both an Inverted Repeat Lacking Clade and a Phaseoloid Legume Host

Although Medicago sativa forms highly effective symbioses with the comparatively acid-sensitive genus Ensifer, its introduction into acid soils appears to have selected for symbiotic interactions with acid-tolerant R. favelukesii strains. Rhizobium favelukesii has the unusual ability of being able to nodulate and fix nitrogen, albeit sub-optimally, not only with M. sativa but also with the promiscuous host Phaseolus vulgaris. Here we describe the genome of R. favelukesii OR191 and genomic features important for the symbiotic interaction with both of these hosts. The OR191 draft genome contained acid adaptation loci, including the highly acid-inducible lpiA/acvB operon and olsC, required for production of lysine- and ornithine-containing membrane lipids, respectively. The olsC gene was also present in other acid-tolerant Rhizobium strains but absent from the more acid-sensitive Ensifer microsymbionts. The OR191 symbiotic genes were in general more closely related to those found in Medicago microsymbionts. OR191 contained the nodA, nodEF, nodHPQ, and nodL genes for synthesis of polyunsaturated, sulfated and acetylated Nod factors that are important for symbiosis with Medicago, but contained a truncated nodG, which may decrease nodulation efficiency with M. sativa. OR191 contained an E. meliloti type BacA, which has been shown to specifically protect Ensifer microsymbionts from Medicago nodule-specific cysteine-rich peptides. The nitrogen fixation genes nifQWZS were present in OR191 and P. vulgaris microsymbionts but absent from E. meliloti-Medicago microsymbionts. The ability of OR191 to nodulate and fix nitrogen symbiotically with P. vulgaris indicates that this host has less stringent requirements for nodulation than M. sativa but may need rhizobial strains that possess nifQWZS for N2-fixation to occur. OR191 possessed the exo genes required for the biosynthesis of succinoglycan, which is required for the Ensifer-Medicago symbiosis. However, 1H-NMR spectra revealed that, in the conditions tested, OR191 exopolysaccharide did not contain a succinyl substituent but instead contained a 3-hydroxybutyrate moiety, which may affect its symbiotic performance with Medicago hosts. These findings provide a foundation for the genetic basis of nodulation requirements and symbiotic effectiveness with different hosts