Architecturally diverse proteins converge on an analogous mechanism to inactivate Uracil-DNA glycosylase

Uracil-DNA glycosylase (UDG) compromises the replication strategies of diverse viruses from unrelated lineages. Virally encoded proteins therefore exist to limit, inhibit or target UDG activity for proteolysis. Viral proteins targeting UDG, such as the bacteriophage proteins ugi, and p56, and the HIV-1 protein Vpr, share no sequence similarity, and are not structurally homologous. Such diversity has hindered identification of known or expected UDG-inhibitory activities in other genomes. The structural basis for UDG inhibition by ugi is well characterized; yet, paradoxically, the structure of the unbound p56 protein is enigmatically unrevealing of its mechanism. To resolve this conundrum, we determined the structure of a p56 dimer bound to UDG. A helix from one of the subunits of p56 occupies the UDG DNA-binding cleft, whereas the dimer interface forms a hydrophobic box to trap a mechanistically important UDG residue. Surprisingly, these p56 inhibitory elements are unexpectedly analogous to features used by ugi despite profound architectural disparity. Contacts from B-DNA to UDG are mimicked by residues of the p56 helix, echoing the role of ugi’s inhibitory beta strand. Using mutagenesis, we propose that DNA mimicry by p56 is a targeting and specificity mechanism supporting tight inhibition via hydrophobic sequestration

Resonant Electron Transfer And Excitation In Two-, Three-, And Four- Electron Caq +20 And Vq +23 Ions Colliding With Helium

Significant new evidence is reported for resonant transfer and excitation in ion-atom collisions. This process, which is analogous to dielectronic recombination, occurs when a target electron is captured simultaneously with the excitation of the projectile followed by photon emission. Strong resonant behavior with structure, in agreement with theoretical calculations, is observed in the cross section for projectile K x rays coincident with single electron capture for 100-360-MeV Ca16+,17+,18+20 and 180-460-MeV V19+,20+,21+23 ions colliding with helium. © 1984 The American Physical Society

Protection and fault detection for Lawrence Berkeley Laboratory neutral beam sources

Testing of TFTR neutral beam (NB) sources has begun at the LBL Neutral Beam System Test Facility (NBSTF). Operation at 120 kV, 65 A, 0.5 sec should be achieved soon. Because NB sources spark down frequently during conditioning, the main accelerating (accel) power supply must be interrupted within a few microseconds to avoid degrading the voltage holding capability, or even the damaging, of the NB source. A variety of improper magnitudes and/or ratios of voltages, currents, and times can occur and must be recognized as fault conditions in order to initiate a prompt interruption of the accel power supply. This paper discusses in detail the key signals which must be monitored and the manner in which they are processed in fault detector circuitry for safe operation of LBL NB sources. The paper also reviews the more standard interlocks and protective features recommended for these sources

X-Rays from Accelerated Ion Interactions

We have developed in detail the theory of X-ray line and continuum production due to atomic interactions of accelerated ions, incorporating in our calculations information from a broad range of laboratory measurements. We applied our calculations to the Orion region from which nuclear gamma-ray lines were observed with the COMPTEL instrument on CGRO. The accelerated particles which produce this gamma-ray emission via nuclear reactions also produce X-ray lines via atomic interactions. We predict strong line emission in the range from 0.5 to 1 keV, mainly due to de-excitations in fast O ions. While much of the diffuse X-ray emission observed with ROSAT from Orion could be due to accelerated ions, the current X-ray data do not provide unambiguous signatures for such an origin. If future observations with high spectral resolution would confirm the predicted X-rays, the combined analysis of the X-ray and gamma-ray data will set important constraints on the origin of the accelerated particles and their interaction model.Comment: 26 pages, 14 figure