An Analysis of California Pharmacy and Medical Students\u27 Dietary and Lifestyle Practices

Objective. To assess dietary and lifestyle practices of pharmacy and medical students in California and investigate whether they adhered to behaviors consistent with current dietary and exercise guidelines. Methods. The Block Brief 2000 Food Frequency Questionnaire and a supplemental survey assessing demographics, exercise, and dietary behaviors were administered to students across 10 California pharmacy and medical schools. Results. While the majority of students consumed sodium/day (73%) and dietary cholesterol/day (84%), only 50% had a saturated fat intake ≤10% total kcal, 13% met fiber intake goals, 10% consumed ≥8 servings/day of fruit and vegetables, and 41% exercised ≥150 minutes/week. The largest barrier to consuming a healthful diet was lack of time. Conclusion. A high proportion of pharmacy and medical students in California did not meet many of the dietary and physical activity recommendations. Health care programs may benefit from implementing nutrition and lifestyle education in their curriculum

A Randomized, Controlled Trial on the Effects of Almonds on Lipoprotein Response to a Higher Carbohydrate, Lower Fat Diet in Men and Women with Abdominal Adiposity.

BACKGROUND: Almonds have been shown to lower LDL cholesterol but there is limited information regarding their effects on the dyslipidemia characterized by increased levels of very low density lipoproteins (VLDL) and small, dense low-density lipoprotein (LDL) particles that is associated with abdominal adiposity and high carbohydrate intake. The objective of the present study was to test whether substitution of almonds for other foods attenuates carbohydrate-induced increases in small, dense LDL in individuals with increased abdominal adiposity. METHODS: This was a randomized cross-over study of three 3wk diets, separated by 2wk washouts: a higher-carbohydrate (CHO) reference diet (CHO RESULTS: Relative to the CHO CONCLUSION: Our analyses provided no evidence that deriving 20% E from almonds significantly modifies increases in levels of small, dense LDL or other plasma lipoprotein changes induced by a higher carbohydrate low saturated fat diet in individuals with increased abdominal adiposity. TRIAL REGISTRATION: Clinicaltrials.gov NCT01792648

The serine protease inhibitor serpinE2 is a novel target of ERK signaling involved in human colorectal tumorigenesis

<p>Abstract</p> <p>Background</p> <p>Among the most harmful of all genetic abnormalities that appear in colorectal cancer (CRC) development are mutations of KRAS and its downstream effector BRAF as they result in abnormal extracellular signal-related kinase (ERK) signaling. In a previous report, we had shown that expression of a constitutive active mutant of MEK1 (caMEK) in normal rat intestinal epithelial cells (IECs) induced morphological transformation associated with epithelial to mesenchymal transition, growth in soft agar, invasion and metastases in nude mice. Results from microarrays comparing control to caMEK-expressing IECs identified the gene encoding for serpinE2, a serine protease inhibitor, as a potential target of activated MEK1.</p> <p>Results</p> <p>1- RT-PCR and western blot analyses confirmed the strong up-regulation of serpinE2 expression and secretion by IECs expressing oncogenic MEK, Ras or BRAF. 2- Interestingly, serpinE2 mRNA and protein were also markedly enhanced in human CRC cells exhibiting mutation in <it>KRAS </it>and <it>BRAF</it>. 3- RNAi directed against serpinE2 in caMEK-transformed rat IECs or in human CRC cell lines HCT116 and LoVo markedly decreased foci formation, anchorage-independent growth in soft agarose, cell migration and tumor formation in nude mice. 4- Treatment of CRC cell lines with U0126 markedly reduced <it>serpinE2 </it>mRNA levels, indicating that expression of <it>serpinE2 </it>is likely dependent of ERK activity. 5- Finally, Q-PCR analyses demonstrated that mRNA levels of serpinE2 were markedly increased in human adenomas in comparison to healthy adjacent tissues and in colorectal tumors, regardless of tumor stage and grade.</p> <p>Conclusions</p> <p>Our data indicate that serpinE2 is up-regulated by oncogenic activation of Ras, BRAF and MEK1 and contributes to pro-neoplastic actions of ERK signaling in intestinal epithelial cells. Hence, serpinE2 may be a potential therapeutic target for colorectal cancer treatment.</p

Crecimiento y condición de juveniles de lenguado (Solea solea L.) como indicadores de calidad de hábitat en áreas de cría costeras y estuáricas del Golfo de Vizcaya con énfasis en zonas expuestas al vertido del Erika

Indicators of growth and condition were used to compare the habitat quality of nurseries of juvenile sole (Solea solea L.) in the Bay of Biscay, based on one survey in 2000. The four biological indicators are poorly correlated with each other, suggesting that no single measure may give an adequate description of fish health and of its habitat’s quality. Growth indicators showed significant differences between northern and southern areas. Juveniles from the two southernmost nurseries, the Gironde estuary and the Pertuis Antioche, displayed significant lower otolith increment widths and mean sizes. These differences were inversely related to water temperature and unrelated to genetic or age differences, and are unlikely to be due to limiting trophic conditions in the nurseries. Hence, they may be considered in terms of differences in habitat quality and potential anthropogenic impacts. Condition indices do not show this north-south pattern but highlight low condition values in the Pertuis Antioche. Short-term and fluctuating biochemical indicators such as RNA/DNA ratios appeared to be unreliable over a long-term study, while morphometric indices seemed to be relevant, complementary indicators as they integrate the whole juvenile life-history of sole in the nurseries. The growth and condition indices of juveniles in September 2000 from nursery grounds exposed to the Erika oil spill in December 1999 were relatively high. These results lead us to suggest that there was no obvious impact of this event on the health of juvenile sole and on the quality of the exposed nursery grounds a few months after the event.Se usaron indicadores de crecimiento y condici&oacute;n de lenguados juveniles (Solea solea L.) para comparar la calidad del h&aacute;bitat de &aacute;reas de cr&iacute;a en el Golfo de Vizcaya basados en un muestreo de 2000. Los cuatro indicadores biol&oacute;gicos mostraron bajas correlaciones entre s&iacute;, lo que sugiere que no existe una medida &uacute;nica para describir adecuadamente el estado de los peces y la calidad de su h&aacute;bitat. Los indicadores de crecimiento mostraron diferencias significativas entre las &aacute;reas septentrionales y meridionales. Los juveniles de las &aacute;reas situadas m&aacute;s al sur: el estuario del Garona y Pertuis Antioche mostraron incrementos de otolitos y tama&ntilde;os medios significativamente inferiores. Estas diferencias est&aacute;n inversamente relacionadas con la temperatura del agua, pero no con diferencias gen&eacute;ticas o de edad, y no es probable que se deba a una condici&oacute;n tr&oacute;fica limitante en las &aacute;reas de cr&iacute;a. Por tanto, pueden considerarse en t&eacute;rminos de calidad del h&aacute;bitat y el potencial impacto antropog&eacute;nico. Los &iacute;ndices de condici&oacute;n no muestras este patr&oacute;n norte-sur sino que destacan los bajos valores de condici&oacute;n en Pertuis Antioche. Los indicadores bioqu&iacute;micos variables de corto t&eacute;rmino como las relaciones RNA/DNA no parecieron fiables para estudios de mayor escala, mientras que los &iacute;ndices morfom&eacute;tricos parecen ser relevantes y complementarios ya que integran toda el per&iacute;odo juvenil de la vida de los lenguados en sus &aacute;reas de cr&iacute;a. Los valores de crecimiento y condici&oacute;n de juveniles en septiembre de 2000 en &aacute;reas de cr&iacute;a expuestas al vertido de crudo del Erika en diciembre de 1999 fueron relativamente elevadas. Estos resultados sugieren que no hubo un impacto obvio de este evento sobre la salud de los juveniles de lenguado ni en la calidad de las &aacute;reas de cr&iacute;a expuestas transcurridos unos pocos meses despu&eacute;s del vertido

Impact of Dietary Resistant Starch on the Human Gut Microbiome, Metaproteome, and Metabolome

Diet can influence the composition of the human microbiome, and yet relatively few dietary ingredients have been systematically investigated with respect to their impact on the functional potential of the microbiome. Dietary resistant starch (RS) has been shown to have health benefits, but we lack a mechanistic understanding of the metabolic processes that occur in the gut during digestion of RS. Here, we collected samples during a dietary crossover study with diets containing large or small amounts of RS. We determined the impact of RS on the gut microbiome and metabolic pathways in the gut, using a combination of “omics” approaches, including 16S rRNA gene sequencing, metaproteomics, and metabolomics. This multiomics approach captured changes in the abundance of specific bacterial species, proteins, and metabolites after a diet high in resistant starch (HRS), providing key insights into the influence of dietary interventions on the gut microbiome. The combined data showed that a high-RS diet caused an increase in the ratio of Firmicutes to Bacteroidetes, including increases in relative abundances of some specific members of the Firmicutes and concurrent increases in enzymatic pathways and metabolites involved in lipid metabolism in the gut

A two years informal learning experience using the Thymio robot

Technology is playing an increasing role in our society. Therefore it becomes important to educate the general public, and young generations in particular, about the most common technologies. In this context, robots are excellent education tools, for many reasons: (i) robots are fascinating and attract the attention of all population classes, (ii) because they move and react to their environment, robots are perceived as close to living beings, which make people attracted and attached to them, (iii) robots are multidisciplinary systems and can illustrate technological principles in electronics, mechanics, computer and communication sciences, and (iv) robots have many applications fields: medical, industrial, agricultural, safety ... While several robots exist on the market and are used for education, entertainment or both, none fits with the dream educational tool: promoting creativity and learning, entertaining, cheap and powerful. We addressed this goal by developing the Thymio robot and distributing it during workshops over two years. This paper describes the design principles of the robot, the educational context, and the analysis made with 65 parents after two years of use. We conclude the paper by outlining the specifications of a new form of educational robot