Best British bonces

This output is an artefact, an artist’s book containing a practice-led history of British cartoonists. Research Process: This research investigates the political elasticity of ‘Britishness’, exploring the semiotics of humour, and how select cartoonists represented the ‘Britishness’ of their childhood. It is researching perceptions of humour and the potential revival of British humour comics. The results of this investigation were represented through a comic format using drawing. Research Insights: The project found that there are modes of humour that are no longer acceptable due to a shift in societal values. The project tries to address what is humour now and the potential for the revival of such aesthetics. Dissemination: The project was disseminated via publication, The Zine, via exhibition and a series of workshops aimed at young people

Match running performance and physical fitness in youth soccer players : a longitudinal study

University of Technology Sydney. Faculty of Health.This study examined whether substantial changes in either maximal sprinting speed (MSS) or maximal aerobic speed (Vvam-Eval) are related to changes in match running performance activity during match play in highly-trained young soccer players. A retrospective longitudinal research design was used where physical fitness and match analysis data were collected. Data from 44 players (U13-U18; fullbacks [FB, n=12], centre-backs [CB, n=12], mid-fielders [MD, n=11], wide-midfielders [WM, n=5], strikers [S, n=4]) who had substantial changes in either MSS or Vvam-Eval throughout 2 consecutive testing periods (~3 months) were included in the final analysis. For each player, time-motion analyses were performed using a global positioning system (1-Hz) during 2-10 international level games played within 1-2 months from/to each testing period of interest. Match activity profiles were described using both absolute and relative zones. Absolute match running activities were defined as meters per min (m·min-1), low-intensity activities (LIA), high-intensity running (HIR), very high-intensity running (VHIR) and sprint activities (SPRT), where relative match activities were categorized into 5 intensity zones in relation to individual MSS and MAS. Improvements in both MSS and Vvam-Eval were likely associated with either non-substantial or lower magnitude changes in match running performance variables and between playing positions. While in response to using relative thresholds, measures were either unchanged or decreased substantially in response to an increase in MSS and/or MAS. Collectively, the results demonstrate that in match running activities during games do not necessarily match those in physical fitness in highly trained young soccer players. Game tactical and strategic requirements are likely to modulate on-field players’ activity patterns independently of players’ physical capacities

The Fourth-Century AD Expansion of the Graeco-Roman Settlement of Karanis (Kom Aushim) in the Northern Fayum*

The Graeco-Roman town of Karanis, founded during the Ptolemaic Period in the north-eastern Fayum in the third century bc and long thought to have been abandoned in the third century ad actually saw a substantial expansion during the fourth century AD. With the creation of an 3 extension towards the west and the expansion of the eastern part, the town grew in both directions. We argue that this expansion may be related to a sudden rise of the water level in Lake Moeris (Lake Qarun), perhaps linked to a catastrophic dam breach in the eastern part of the Fayum, and the subsequent relocation of the inhabitants of the low-lying settlements north and east of the lake

The Application of CRISPR Technology to High Content Screening in Primary Neurons

Axon growth is coordinated by multiple interacting proteins that remain incompletely characterized. High content screening (HCS), in which manipulation of candidate genes is combined with rapid image analysis of phenotypic effects, has emerged as a powerful technique to identify key regulators of axon outgrowth. Here we explore the utility of a genome editingapproach referred to as CRISPR (Clustered Regularly Interspersed Palindromic Repeats) for knockout screening in primary neurons. In the CRISPR approach a DNA-cleaving Cas enzyme is guided to genomic target sequences by user-created guide RNA (sgRNA), where it initiates a double-stranded break that ultimately results in frameshift mutation and loss of protein production. Using electroporation of plasmid DNA that co-expresses Cas9enzyme and sgRNA, we first verified the ability of CRISPR targeting to achieve protein-level knockdown in cultured postnatal cortical neurons. Targeted proteins included NeuN (RbFox3) and PTEN, a well-studied regulator of axon growth. Effective knockdown lagged at least four days behind transfection, but targeted proteins were eventually undetectable by immunohistochemistry in \u3e 80% of transfected cells. Consistent with this, anti-PTEN sgRNA produced no changes in neurite outgrowth when assessed three days post-transfection. When week-long cultures were replated, however, PTEN knockdown consistently increased neurite lengths. These CRISPR-mediated PTEN effects were achieved using multi-well transfection and automated phenotypic analysis, indicating the suitability of PTEN as a positive control for future CRISPR-based screening efforts. Combined, these data establish an example of CRISPR-mediated protein knockdown in primary cortical neurons and its compatibility with HCS workflows

Prospectus, January 14, 1998

https://spark.parkland.edu/prospectus_1998/1000/thumbnail.jp

A comparison of the knowledge and attitudes of concussion within higher and lower leagues of the community rugby union game

Objectives: The Rugby Football Union (RFU) HEADCASE initiative aims to educate coaches, referees, and players on concussion. Concussion in community rugby is under-reported. Therefore, players can play a role in concussion safety if they have adequate knowledge and safe attitudes. The study aimed to evaluate and compare the concussion knowledge and attitudes and Return to Play (RTP) of the community rugby union game. Design: A descriptive, cross-sectional study. Participants: Participants (n = 87) were from level 4 or level 8 of the English rugby union league system. Main outcome measures: A modified Rosenbaum Concussion Knowledge and Attitudes Survey-Student Version (RoCKAS-ST) questionnaire was distributed to semi-professional and amateur clubs to evaluate players concussion knowledge index (CKI) and concussion attitudes and behaviours index (CAI) regarding RTP. Results: No statistically significant differences existed relating to knowledge, behaviours or attitude. Some worrying gaps in knowledge were identified. Conclusions: Findings show that community rugby union players are knowledgeable about concussion and have shown safe attitudes, however this doesn't always translate to safe behaviours. There are some gaps in their knowledge that must be addressed relating to coma and coherence of the athlete and in symptom recognition relating to sleep disturbances

Prospectus, April 8, 1998

https://spark.parkland.edu/prospectus_1998/1011/thumbnail.jp

Prospectus, April 15, 1998

https://spark.parkland.edu/prospectus_1998/1012/thumbnail.jp

Prospectus, February 18, 1998

https://spark.parkland.edu/prospectus_1998/1005/thumbnail.jp