Correlation analysis between GDF-15 and FGF-21.

<p>serum levels in samples from all patient groups taken as a whole (A), group 1(B), group 2 (C) and group 3(D).</p

Correlation analysis between GDF-15 and FGF-21 protein levels.

<p>in the conditioned medium of C2C12 cells treated with respiratory chain inhibitors.</p

Effects of experimentally induced mitochondrial dysfunction on GDF-15 mRNA expression and GDF15 protein release in muscle cells.

<p>GDF15 mRNA and FGF21 mRNA levels in C2C12 myotubes (A) or LHCN myotubes (B), and correlation between GDF-15 mRNA levels and FGF21 mRNA levels (C) in the experimental settings in C2C12 cells (up) and LHCN cells (down). GDF-15 protein concentrations in C2C12 cell culture medium (D). Bars are means ±S.E.M. from 4–6 independent experiments. *p <0.05, **p <0.01, ***p <0.001, relative to untreated controls. ^# p <0.05, relative to corresponding condition non-treated with Trolox. R and P values are shown in the correlation panel C.</p

Correlation analysis between GDF-15 and FGF-21.

<p>serum levels in samples from all patient groups taken as a whole (A), group 1(B), group 2 (C) and group 3(D).</p

Serum concentration of GDF-15 and FGF-21 in patients and controls.

<p>Serum concentration of GDF-15 and FGF-21 in patients and controls.</p

Receiving Operating Curve.

<p>analysis for GDF-15 and FGF-21 considering patients in groups 1, 2 and 3 together (A) or patients group 1(B), group 2 (C) and group 3(D) separately.</p

Histogram representing the percentage of patients in each group with both GDF-15 and FGF-21above cut-off values, GDF-15 or FGF-21 elevated or both factors within normal values.

<p>Histogram representing the percentage of patients in each group with both GDF-15 and FGF-21above cut-off values, GDF-15 or FGF-21 elevated or both factors within normal values.</p

GDF-15 and FGF-21 circulating concentrations.

<p>Data are represented as the median +/- the interquartile range. *p< 0.05, **p < 0.01, *** p< 0.001 (U-Mann-Whitney test).</p

Diagnostic performance of GDF-15 (A) and FGF-21 (B) for a cut-off value of 550pg/ml and 300pg/ml respectively.

<p>95% confidence intervals are indicated in brackets.</p