Autocrine PDGF stimulation in malignancies

Platelet-derived growth factor (PDGF) isoforms are important mitogens for different types of mesenchymal cells, which have important functions during the embryonal development and in the adult during wound healing and tissue homeostasis. In tumors, PDGF isoforms are often over-expressed and contribute to the growth of both normal and malignant cells. This review focuses on tumors expressing PDGF isoforms together with their tyrosine kinase receptors, thus resulting in autocrine stimulation of growth and survival. Patients with such tumors could benefit from treatment with inhibitors of either PDGF or PDGF receptors

Collection of triatomines from sylvatic habitats by a Trypanosoma cruzi-infected scent detection dog in Texas, USA.

BackgroundTriatomine insects, vectors of the etiologic agent of Chagas disease (Trypanosoma cruzi), are challenging to locate in sylvatic habitats. Collection techniques used in the United States often rely on methods to intercept seasonally dispersing adults or on community scientists' encounters. Neither method is suited for detecting nest habitats likely to harbor triatomines, which is important for vector surveillance and control. Furthermore, manual inspection of suspected harborages is difficult and unlikely to reveal novel locations and host associations. Similar to a team that used a trained dog to detect sylvatic triatomines in Paraguay, we worked with a trained scent detection dog to detect triatomines in sylvatic locations across Texas.Principle methodology/findingsZiza, a 3-year-old German Shorthaired Pointer previously naturally infected with T. cruzi, was trained to detect triatomines. Over the course of 6 weeks in the fall of 2017, the dog and her handler searched at 17 sites across Texas. The dog detected 60 triatomines at 6 sites; an additional 50 triatomines were contemporaneously collected at 1 of these sites and 2 additional sites without the assistance of the dog. Approximately 0.98 triatomines per hour were found when only humans were conducting searches; when working with the dog, approximately 1.71 triatomines per hour were found. In total, 3 adults and 107 nymphs of four species (Triatoma gerstaeckeri, Triatoma protracta, Triatoma sanguisuga, and Triatoma indictiva) were collected. PCR testing of a subset revealed T. cruzi infection, including DTUs TcI and TcIV, in 27% of nymphs (n = 103) and 66% of adults (n = 3). Bloodmeal analysis of a subset of triatomines (n = 5) revealed feeding on Virginia opossum (Didelphis virginiana), Southern plains woodrat (Neotoma micropus), and eastern cottontail (Sylvilagus floridanus).Conclusion/significanceA trained scent detection dog enhanced triatomine detections in sylvatic habitats. This approach is effective at detecting nidicolous triatomines. Control of sylvatic sources of triatomines is challenging, but this new knowledge of specific sylvatic habitats and key hosts may reveal opportunities for novel vector control methods to block the transmission of T. cruzi to humans and domestic animals

Training and field sites across Texas where triatomine scent detection efforts by a trained dog were conducted.

Dog scent detection efforts were conducted at locations where kissing bugs had been submitted by community scientists or collected by our field team previously. Initial training for this study took place in College Station (triangle). Triatomines were found at 8 of the 17 sites searched. Previous records of triatomines are based on our field collections and submission to the Kissing Bug Community Science Program [18]. Base map created using QGIS [49] with Texas counties data file from https://data.texas.gov/dataset/Texas-Counties-Map/48ag-x9aa [50].</p

Triatomine detection dog in the field.

(A) ‘Sit’ was the primary indication of a detection. (B) ‘Dig’ was the secondary indication of a detection. (C) Blood fed nymphs found at a south Texas site, in a small pile of railroad ties in the corner of a horse pen where rabbits had been seen. (D) Woodpile where nymphs were found.</p

Bloodmeal analysis of triatomines found by a scent detection dog.

Bloodmeal analysis of triatomines found by a scent detection dog.</p

Search success by detection type and calculation of specimens found per hours searched.

Search success by detection type and calculation of specimens found per hours searched.</p