Effect of Testosterone Enanthate Modeling of Polycystic Ovary on Liver Irs-2 mRNA Expression in Rats: A Brief Report

هناك العديد من النماذج الحيوانية لتكيس المبايض (PCO). يعد استخدام إينونثات التستوستيرون الخارجي إحدى طرق تحريض هذه النماذج. ومع ذلك ، يجب أيضًا دراسة تحريض مقاومة الأنسولين في تقنيات النماذج. لذلك ، تهدف الدراسة الحالية إلى التحقق من تعبير ركيزة مستقبلات الأنسولين (Irs) -2 mRNA في أنسجة الكبد لنموذج PCO الفئران. تم تقسيم تسعة عشر من قئران ويستار إلى ثلاث مجموعات. (1) تلقت مجموعة نمذجة PCO (N = 7) يوميًا 1.0 مجم / 100 جرام من إينونثات التستوستيرون المذاب في زيت الزيتون جنبًا إلى جنب مع الوصول الحر الى ماء الدكستروز ذي  التركيز 5 ٪ ، (2) مجموعة المركبات (N = 6) ، والتي تم التعامل معها مثل مجموعة PCO ، لكنهم لم يتلقوا إينونثات التستوستيرون، (3) مجموعة التحكم (N = 6) مع الرعاية المنتظمة. تم حقن جميع الحيوانات داخل الصفاق لمدة 14 يومًا. تمت دراسة التعبير عن Irs-2 mRNA باستخدام PCR في الوقت الفعلي وتم الإبلاغ عن تغييرات الطي (FC). تم اعتبار متوسط التعبير في المجموعة الضابطة بمثابة المعيار. تم العثور على حوالي 13.4 ٪ تقليل التعبير في مجموعة PCO (FC = 0.874 ، قيمة P = 0.043). لم يتم العثور على انخفاض كبير في مجموعة المركبات (FC = 0.951 ، قيمة P = 0.076). ومع ذلك ، لم يُظهر تحليل التباين فرقًا معنويًا بين جميع مجموعات الدراسة (قيمة P = 0.085). قد يؤدي النموذج الحالي لـ PCO إلى مقاومة الأنسولين على مستوى الكبد بحجم تأثير منخفض عن طريق تقليل تعبير mRNA عن Irs-2. يُقترح دراسة الجينات والجزيئات المعنية في الأنسجة الأخرى لنماذج حيوانية PCO.There are many animal models for polycystic ovary (PCO); using exogenous testosterone enanthate is one of the methods of induction of these models. However, induction of insulin resistance should also be studied in the modeling technics. Therefore, the present study aims to investigate the expression of insulin receptor substrate (Irs)-2 mRNA in the liver tissue of rat PCO model. Nineteen Wistar rats were divided into three groups; (1) PCO modeling group (N =7) received daily 1.0 mg/100g testosterone enanthate solved in olive oil along with free access dextrose water 5%, (2) vehicle group (N =6), which handled like the PCO group, but did not receive testosterone enanthate, (3) control group (N =6) with standard care. All the animals were administered via intra-peritoneal injection for 14 days. Expression of Irs-2 mRNA was studied with real-time PCR and fold changes (FC) were reported. The average of expression in the control group was considered as the calibrator. About 13.4% expression reduction was found in the PCO group (FC =0.874, P-value =0.043). No significant reduction was found in the vehicle group (FC =0.951, P-value =0.076). However, analysis of variance did not show a significant difference between all the groups of study (P-value =0.085). The present model of PCO might induce insulin resistance at liver level with a low effect size via reduction in the mRNA expression of Irs-2. Study of the involved genes and molecules in other tissues of PCO animal models is suggested

The effect of progesterone treatment after ovarian induction on endometrial VEGF gene expression and its receptors in mice at pre-implatation time

Objective(s): Progestrone is a prequisite for pre-implantation angiogenesis and induce decidual angiogenesis. It is unknown the effect of progestrone administration on the endometrium of hyperstimulated mice at pre-implantation time. Material and Methods: Adult female NMRI mice were divided in three groups [control group, ovarian stimulated group and progestrone treated mice after ovarian stimulation]. Uterine horn samples removed at pre-implantation time in each group. Motic image Plus 2 software was used to assess the quantitative vascular parameters of endometrium. Gene expression was determined for vascular endothelial growth factor (VEGF), FMS-like tyrosine kinase (FLT) and Kinase insert domain protein receptor (FLK)genes using the real time PCR method. Data analysis was done with LinReg PCR and Rest-RG software. Results: Comparison between progestrone treated mice after ovarian stimulation with control group showed that increase in rate of VEGF gene expression [0.775] and decrease in rate of FLK [6.072] and FLT [1.711] gene expression. Analysis of the data on quantitative vascular parameters were indicated remarkable increase in quantitative vascular parameters of progestrone treated mice compare to control group. Conclusion: Biological effect of progestrone on the vascular changes after ovarian stimulation resulted in an increase in VEGF receptors experession, it seems that induced angiogenesis by progesterone could result in better condition for implantation

Effect of progesterone administration following ovarian stimulation on mast cell count and histamine level in mice ovary

Introduction: Induction of ovulation results in some changes in ovary including the tissue immune cells. Administration of progesterone following induction of ovulation may ameliorate some of these changes. The present study was performed to investigate the effect of this administration of progesterone on mast cell count and tissue histamine level in mice ovary at pre-implantation time. Methods: An experimental study on NMRI mice was conducted. The groups of study were control group, induction of ovulation group and a group for administration of progesterone after induction of ovulation. Mast cells count was through toluidine blue staining and tissue histamine level measure was through spectrophotometry. Results: Mean of mast cell count was significantly different between the groups (P <0.001). Pairwise comparisons showed that induction group had significantly higher mast cells in comparison to control group (P =0.039) showing the effect of ovulation induction on mast cell count. The count was higher in progesterone group in comparison to induction group (P =0.020). Mean of histamine level was significantly different between the groups (P <0.001). Induction group had significantly higher histamine level in comparison to control group (P <0.001). However, histamine level was not significantly different between induction and histamine groups (P =0.998). Conclusion: Ovulation induction results in increased in ovary mast cell population and histamine level. However, progesterone could not ameliorate this change. Further studies should be performed to find further roles of mast cells and histamine in ovary

Effect of 3, 4-Dihydroxyphenylethanol on Antioxidant Enzymes Activity and Malondialdehyde Level in HCT-116 Cell Line

Background: There is much evidence-based research on the anti-cancerous effect of antioxidants. Dihydroxyphenylethanol is a potent antioxidant that has several activities, such as oxidant stress control, cell proliferation inhibitory activity, and apoptosis induction. The present study aimed to evaluate the effect of dihydroxyphenylethanol on antioxidant enzyme activity and malondialdehyde level in the HCT-116 cell line. Materials and Methods: In this study, a human colorectal cancer cell line HCT-116 was treated with different concentrations of 3, 4-dihydroxyphenylethanol (50, 100, 150, and 200 μM) for 24 h. Then, the level of malondialdehyde and activity of enzymes of catalase, superoxide dismutase, and glutathione peroxidase were measured by colorimetric methods. Results: The results showed that 3,4-dihydroxyphenylethanol administration resulted in significant reduction of the malondialdehyde concentration and also significant increase of the antioxidant enzymes (e.g., catalase, superoxide dismutase, and glutathione peroxidase) in the treatment groups compared to the control group (P<0.05). Conclusion: Overall, 3, 4-dihydroxyphenylethanol may result in reduction of oxidative stress on HCT-116 line through changes in concentration of the malondialdehyde and antioxidant enzymes activity

Effect of Selenium on Expression of Apoptosis-Related Genes in Cryomedia of Mice Ovary after Vitrification

Introduction. Freezing of ovarian tissue is used for preservation of fertility. The freezing-thawing process is accompanied by oxidative stress and induction of apoptosis. Apoptosis is a complex process that has been studied in animal models. The present study was aimed at investigating the effect of selenium on suppression of apoptosis during vitrification-thawing process of mice ovary via studying expression of apoptosis-related genes, and also, we aimed to design statistical models for the roles of single genes and gene-gene interactions in suppression of apoptosis. Methods. A total of 10 right ovary samples from 10 mice were randomly divided into two groups of selenium treatment (at dose 5 μg/ml sodium selenite, through adding to the media) and control group. Vitrification-thawing process was done according to the existed protocols. Real-time PCR was used for gene expression study. The apoptosis gene profile included P53, Bax, Fas, and Bcl-2. General linear model was applied to study single gene associations and gene-gene interactions. Results. From the studied genes, P53 showed a significant downregulation in the selenium group in comparison to the control group (∆∆CT=1.96; P=0.013; relative expression RE=0.28). Bcl-2 showed a significant upregulation in the selenium group in comparison to the control group (∆∆CT=−2.49; P<0.001; RE=3.49). No significant result was found for other genes. According to the multiple models, Bcl-2 showed a protective single gene association (beta=−0.33; P=0.032), and Fas∗Bcl-2 interaction was significantly positive (beta=0.19; P=0.036). Conclusion. Addition of selenium to cryomedia of vitrification-thawing process could reduce the apoptosis induced by freezing-thawing stress in mice ovary via downregulation of P53 and upregulation of Bcl-2 at transcription level. Multivariable statistical models should be performed in future researches to study biological systems

Protective Effect of Thyme Honey against Valproic Acid Hepatotoxicity in Wistar Rats

Introduction. Valproic acid is a medication most commonly used in the treatment of emotional and neurological depression, psychological imbalances, epilepsy, and bipolar disorder. Dark honey, like thyme honey, contains more antioxidant compounds than other samples. The purpose of this study was to evaluate the effect of thyme honey on the potential hepatic effects of valproic acid. Methods. In this study, 48 male rats were randomly divided into 8 groups (n=6): G1 (control): healthy rats (normal saline 0.9%), G2: thyme honey (1 g/kg), G3: thyme honey (2 g/kg dose), G4: thyme honey (3 g/kg dose), G5: VPA (500 mg/kg), G6: VPA (500 mg/kg) and thyme honey (1 g/kg), G7: VPA (500 mg/kg) and thyme honey (2 g/kg dose), and G8: VPA (500 mg/kg) and thyme honey (3 g/kg dose). Groups G1 to G5 received the drug for 28 days. On day 14, administration of thyme honey for G6 to G8 groups was carried out using gavage until day 28. VPA was administered one hour after honey. To carry out the biochemical evaluation, blood samples were collected from all the groups and their serums were used for MDA, TAC, and liver enzymes (AST, ALT, and GGT). Tissue samples of each rat were also removed for histological studies with hematoxylin-eosin and Masson’s trichrome staining. Results. The use of thyme honey significantly improved the histopathological parameters of the liver tissue, including hypertrophic degeneration and nucleus alteration, expansion of sinusoids, fibrosis and hepatic necrosis, and inflammation as well as hypertrophy of Kupffer cells. In the groups receiving VPA, the rate of lipid peroxidation increased, which indicates the destruction of the liver cell membrane due to drug consumption. TAC levels also increased following increase in thyme honey dosage (p≤0.05). The results of liver enzyme analysis showed a decrease in AST and ALT levels in the G6 group and a decrease in GGT level in the G8 group (p≤0.05). Conclusion. Based on the results of this study, it seems that high percentage of antioxidants in thyme honey enabled it to improve hepatic complications and reduce the rate of hepatocellular destruction

The effect of low dose (7.5 mg/kg) of Aspirin on ovary tissue during implantation period in mice (NMRI)

Background: Aspirin is a non steroidal medicine that effects on prostaglandin, follicles, ovulation and corpus luteum. The aim of this study was to evaluate the effect of low (7.5 mg/kg) dose of Aspirin on the ovarian tissue during implantation period. Materials and Methods: Females NMRI mice 6-8 weeks old were divided into control and experimental groups. These groups were rendered pseudopregnant then experimental group injected with low dose of Aspirin until 4.5th day. The mice were scarified with cervical dislocation and then the samples were obtained from the ovary in each group. Then ovarian weight and volume balance were measured and paper section stained with H&E method. Results: Our results showed that weight of ovary in control group was 3.1±0.077g and in experimental group was 3.5±0.24g (p<0.05). Volume of ovary in control group was 8.70±0.19 mm3, and in experimental group was 9.20±0.17 mm3 (p<0.05). Number of corpus luteum in control group was 6.2±0.15, and in experimental group was 12.21±0.15 (p<0.05). In experimental group vascularity of ovary had increased in comparison with control group. Conclusion: The low dose of Aspirin (7.5mg/kg) increased the rate of corpus luteum and vascularity of ovary . Low dose of aspirin had positive effect on pregnancy during implantation period

Reporting a lethal drug interaction occurred during evaluation of insulin resistance in mice polycystic ovary model

No abstrac

Reporting a lethal drug interaction occurred during evaluation of insulin resistance in mice polycystic ovary model

No abstrac