Pseudomonas campi sp. nov., a nitrate-reducing bacterium isolated from grassland soil

A novel strain was isolated from grassland soil that has the potential to assimilate ammonium by the reduction of nitrate in the presence of oxygen. Whole genome sequence analysis revealed the presence of an assimilatory cytoplasmic nitrate reductase gene nasA and the assimilatory nitrite reductase genes nirBD which are involved in the sequential reduction of nitrate to nitrite and further to ammonium, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate represents a member of the genus Pseudomonas. The closest phylogenetic neighbours based on 16S rRNA gene sequence analysis are the type strains of Pseudomonas peli (98.17%) and Pseudomonas guineae (98.03%). In contrast, phylogenomic analysis revealed a close relationship to Pseudomonas alcaligenes. Computation of the average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) with the closest phylogenetic neighbours of S1-A32-2T revealed genetic differences at the species level, which were further substantiated by differences in several physiological characteristics. On the basis of these results, it was concluded that the soil isolate represents a novel species of the genus Pseudomonas, for which the name Pseu-domonas campi sp. nov. (type strain S1-A32-2T=LMG 31521T=DSM 110222T) is proposed

The role of mental disorders in the risk and speed of transition to alcohol use disorders among community youth

Background Among adolescents and young adults with DSM-IV alcohol use disorders (AUDs), there are inter-individual differences in the speed of transition from initial alcohol use (AU) to AUD. AUDs are highly co-morbid with other mental disorders. The factors associated with rapid transition from first AU to AUD remain unknown and the role of mental disorders in rapid transitions is unclear. Given this background we examined (1) whether prior anxiety, mood, externalizing and non-alcohol substance use disorders are related to the risk and speed of transition from first AU to DSM-IV alcohol abuse (AA) and alcohol dependence (AD) and (2) whether early age of onset of prior mental disorders (PMDs) is a promoter of rapid transition. Method A total of 3021 community subjects (97.7% lifetime AU) aged 14-24 years at baseline were followed up prospectively for up to 10 years. AU and mental disorders were assessed with the DSM-IV/M-CIDI. Results Among subjects with lifetime AU, several PMDs, such as specific phobia, bipolar disorder and nicotine dependence, were associated with an increased risk of AUD independent of externalizing disorders. Associations of PMDs with the speed of transition to AUDs were mostly weak and inconsistent. Only social phobia and externalizing disorders were associated with faster transitions to AD even after adjustment for other PMDs. Earlier age of onset of PMD was not associated with rapid transition. Conclusions Mental disorders are associated with the risk of AUD. With the possible exception of social phobia and externalizing disorders, they do not promote rapid transition, even if they occur particularly early. Future research needs to identify factors relevant to rapid transition to AU

Two-Dimensional Laser Diagnostics and Modeling of Counterflow Diffusion Flames

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/86756/1/Sick51.pd

Biomarkers in Urachal Cancer and Adenocarcinomas in the Bladder: A Comprehensive Review Supplemented by Own Data

Urachal cancer (UrC) is a rare but aggressive cancer. Due to overlapping histomorphology, discrimination of urachal from primary bladder adenocarcinomas (PBAC) and adenocarcinomas secondarily involving the bladder (particularly colorectal adenocarcinomas, CRC) can be challenging. Therefore, we aimed to give an overview of helpful (immunohistochemical) biomarkers and clinicopathological factors in addition to survival analyses and included institutional data from 12 urachal adenocarcinomas. A PubMed search yielded 319 suitable studies since 1930 in the English literature with 1984 cases of UrC including 1834 adenocarcinomas (92%) and 150 nonadenocarcinomas (8%). UrC was more common in men (63%), showed a median age at diagnosis of 50.8 years and a median tumor size of 6.0 cm. No associations were noted for overall survival and progression-free survival (PFS) and clinicopathological factors beside a favorable PFS in male patients (p = 0.047). The immunohistochemical markers found to be potentially helpful in the differential diagnostic situation are AMACR and CK34betaE12 (UrC versus CRC and PBAC), CK7, beta-Catenin and CD15 (UrC and PBAC versus CRC), and CEA and GATA3 (UrC and CRC versus PBAC). Serum markers like CEA, CA19-9 and CA125 might additionally be useful in the follow-up and monitoring of UrC

The size, structure and efficiency of Mongolian flocks and herds on degraded grasslands

Since 1990, the number of livestock in Mongolia has more than doubled. In large areas of Mongolia, grassland degradation is now a problem of national concern. A survey was done of 10 herders in two Soums (Altanbulag and Khashaat; heavily and moderately degraded, respectively) to measure the animals (sheep, goats, cattle and horse numbers, sex and weights) and grasslands (species and biomass) every 3 months from April 2017 to December 2018. Rainfall is mostly in summer and mean monthly temperatures vary from –20 to 20°C. Throughout the study, the grassland herbage mass in summer was less than 0.5 t dry matter/ha, below that considered sustainable for the steppe. Herders had 200–1000 sheep equivalents (SE, 50 kg base weight), moved camps 2–4 times each year and stocking rates varied from 0.5–1 SE/ha. Female animals reached their mature weights by 4 years of age (sheep 50 kg, goats 40 kg, cattle 350 kg, horses 300 kg). Animals only grew over summer, but a significant part of that was compensatory gain in animals >1 year old. Over winter, sheep and goats lost 21–29% of their liveweight, cattle and horses 15–30%. The weaning rate was 43–48% across species. The male:female ratio (>2 years old) was sheep 63%, goats 72%, cattle 27% and horses 106%. All animals are managed in common mobs with no particular control of breeding. Areas where the efficiency of livestock production and grassland productivity could be improved are discussed