Genetic Complementation to Identify DNA Elements That Influence Complement Resistance in Leishmania chagasi

Past studies showed that Leishmania spp. promastigotes exhibit differential sensitivity to complement mediated lysis (CML) during development in vitro and in vivo. Leishmania chagasi promastigotes in cultures during logarithmic and stationary growth phases are CML-sensitive or CML-resistant when exposed to human serum, respectively, but only in cultures recently initiated with parasites from infected animals; serially passaged cultures become constitutively CML-sensitive regardless of growth phase. Building on these observations, a genetic screen was conducted to identify novel complement resistance factors of L. chagasi. A cosmid library containing genomic DNA was transfected into a promastigote line previously subjected to \u3e50 serial passages. Selection with human serum for CML resistance yielded 12 transfectant clones. Cosmids isolated from 7 of these clones conferred CML resistance when transfected into an independent, high-passage promastigote culture; at 12% human serum, the mean survival of transfectants was 37% (±11.6%), and that of control transfectants was about 1%. Inserts within the 7 cosmids were unique. Determination of the complete DNA sequence for 1 cosmid indicated that its 32-kilobase insert was 89% identical (overall) to a 31-kilobase region of Leishmania major chromosome 36, which is predicted to encode 6 genes, all of which encode hypothetical proteins

The Origin of Article I, Section 7 of the Washington State Constitution

This Article will demonstrate that history does in fact provide guidance to the intention of the framers when they rejected the language of the Fourth Amendment and adopted the unique language of article I, section 7. Contrary to the Ringer court\u27s assertion, federal and state case law, legal academic articles, and newspaper articles from the late nineteenth century and early twentieth century provide a wealth of information from which the rationale behind the framers\u27 decision to choose the specific language in article I, section 7 can be hypothesized

Democratic cultural policy : democratic forms and policy consequences

The forms that are adopted to give practical meaning to democracy are assessed to identify what their implications are for the production of public policies in general and cultural policies in particular. A comparison of direct, representative, democratic elitist and deliberative versions of democracy identifies clear differences between them in terms of policy form and democratic practice. Further elaboration of these differences and their consequences are identified as areas for further research

Coral reef islands can accrete vertically in response to sea level rise

Increased flooding due to sea level rise (SLR) is expected to render reef islands, defined as sandy or gravel islands on top of coral reef platforms, uninhabitable within decades. Such projections generally assume that reef islands are geologically inert landforms unable to adjust morphologically. We present numerical modeling results that show reef islands composed of gravel material are morphodynamically resilient landforms that evolve under SLR by accreting to maintain positive freeboard while retreating lagoonward. Such island adjustment is driven by wave overtopping processes transferring sediment from the beachface to the island surface. Our results indicate that such natural adaptation of reef islands may provide an alternative future trajectory that can potentially support near-term habitability on some islands, albeit with additional management challenges. Full characterization of SLR vulnerability at a given reef island should combine morphodynamic models with assessments of climate-related impacts on freshwater supplies, carbonate sediment supply, and future wave regimes.</jats:p

Performance evaluation of rail trackbed stiffness: pre and post stabilisation

Excessive deflection of a rail in response to axle loading can lead to discomfort for passengers and increased wear of both railway structures and trains. These oscillations are often caused by poor trackbed stiffness which may be due to either soft subgrade and/or contaminated ballast. A variety of trackbed stabilisation (TBS) techniques are available to remediate soft subgrades and increase the safety of tracks within the railway network. Traditional TBS methods require track removal, which is expensive, disruptive and often inefficient maintenance works. Micro-piling, using screw piles installed between sleepers, is an innovative low disruption TBS technique. This paper investigates the performance of a soft subgrade and contaminated ballast section of rail line in the UK, before and after screw pile TBS. Pre and post remediation, a computer vision-based system was used to measure rail vertical deflections during train passages and then analysed to quantify the trackbed stiffness. Additionally, 3D finite element models are created and validated by the site measurements. The finite element models are used to simulate a range of different scenarios exploring how changes to the TBS piling layout and/or further works, such as ballast improvement could add further improvements or design efficiencies. Site measurements show TBS reduced rail deflection by 20–30%, indicating that micro-piling is an effective technique for soft subgrades. The finite element analysis revealed the efficiency of micro-piling is highly dependent on the conditions of ballast, strength of the ground at the pile toe, and the pile arrangement. When the aforementioned are optimised the rail deflection could be reduced to approximately 50% of the pre TBS condition

Lime stabilisation for earthworks: a UK perspective

Lime stabilisation is a versatile technique applied during earthworks operations. Modern soil recycling units are much more efficient at pulverising fill material and intermixing the added binder/water than machinery available 20 years ago. While supplier innovation adds flexibility to the site working method, specifications have not been sufficiently updated to permit optimal application. This review paper details the physico-chemical changes instigated through the lime-clay soil reaction, updating previous reviews. It aims to assist scientific debate, current practitioners and future specification changes. For example, the application of the minimum 24 h mellowing periods (mandatory to UK specifications) with high reactivity, quicklime powders is concluded to cause increased air voids in the compacted fill. Increased air voids are associated with reduced long-term strength and potential volume change from water ingress, which is of particular concern for sulfate swelling. Shorter mellowing periods and/or use of hydrated lime may lesson this issue; however, a 'one size fits all' approach is discouraged in preference to site-specific methodologies refined to suit the fill material and project requirements. The discussion also summarises working methods which may lower the risk of sulfate swell and defines areas requiring further practical research

NGO Legitimacy: Four Models

The aim of this paper is to examine NGOs’ legitimacy in the context of global politics. In order to yield a better understanding of NGOs’ legitimacy at the international level it is important to examine how their legitimacy claims are evaluated. This paper proposes dividing the literature into four models based on the theoretical and analytical approaches to their legitimacy claims: the market model, social change model, new institutionalism model and the critical model. The legitimacy criteria generated by the models are significantly different in their analytical scope of how one is to assess the role of NGOs operating as political actors contributing to democracy. The paper argues that the models present incomplete, and sometimes conflicting, views of NGOs’ legitimacy and that this poses a legitimacy dilemma for those assessing the political agency of NGOs in world politics. The paper concludes that only by approaching their legitimacy holistically can the democratic role of NGOs be explored and analysed in the context of world politics

A multi-dimensional investigation of self-regulated learning in a blended classroom context : a case study on eLDa MOOC

Online systems such as massive open online courses (MOOCs) are new innovative learning technology in education. With the proliferation of MOOC systems, little has been mentioned about blended MOOC system and how it enhances students’ performance. Blended classroom is a form of learning taking place between two different activities of which one is online and the other is traditional teaching method using bricks and mortal classroom settings. This study reveals the effectiveness of blended classroom teaching for an undergraduate course. The module was embedded in an eLDa MOOC platform, which is a platform for delivery computing concepts, and Python programme course. This research aims to investigate students’ perceptions of self-regulated learning (SRL) habits. A multi-dimensional survey was designed to evaluate each aspect of SRL skills, motivation and attaining better grades within the course. This research analysis explores (a) cognitive process of students improving their self-regulated learning skills (b) potential of students’ preparedness and motivation to engage with the course content in a blended context (c) potential difference in addressing the relation among the methods of engagement and achievement in their weekly assessment results. The research applied an online self-regulated learning questionnaire (OSLQ) as the instrument for measuring the self-regulated learning skills of the students in the learning platform environment. In relation to developing a revised OSLQ to address the use of the instrument to measure self-regulated learning in an online blended classroom context. Data collection process was conducted on a sample of first year undergraduate students who took a seminar module via a blended course format. The results indicate the level of self-regulated learning explored from the measure of the self-regulation in the blended learning environment in this study

'Word from the street' : when non-electoral representative claims meet electoral representation in the United Kingdom

Taking the specific case of street protests in the UK – the ‘word from the street’– this article examines recent (re)conceptualizations of political representation, most particularly Saward’s notion of ‘representative claim’. The specific example of nonelectoral claims articulated by protestors and demonstrators in the UK is used to illustrate: the processes of making, constituting, evaluating and accepting claims for and by constituencies and audiences; and the continuing distinctiveness of claims based upon electoral representation. Two basic questions structure the analysis: first, why would the political representative claims of elected representatives trump the nonelectoral claims of mass demonstrators and, second, in what ways does the ‘perceived legitimacy’ of the former differ from the latter