1,658 research outputs found
Tryptophan metabolism and bacterial commensals prevent fungal dysbiosis in Arabidopsis roots
In nature, roots of healthy plants are colonized by multikingdom microbial communities that include bacteria, fungi, and oomycetes. A key question is how plants control the assembly of these diverse microbes in roots to maintain host–microbe homeostasis and health. Using microbiota reconstitution experiments with a set of immunocompromised Arabidopsis thaliana mutants and a multikingdom synthetic microbial community (SynCom) representative of the natural A. thaliana root microbiota, we observed that microbiota-mediated plant growth promotion was abolished in most of the tested immunocompromised mutants. Notably, more than 40% of between-genotype variation in these microbiota-induced growth differences was explained by fungal but not bacterial or oomycete load in roots. Extensive fungal overgrowth in roots and altered plant growth was evident at both vegetative and reproductive stages for a mutant impaired in the production of tryptophan-derived, specialized metabolites (cyp79b2/b3). Microbiota manipulation experiments with single- and multikingdom microbial SynComs further demonstrated that 1) the presence of fungi in the multikingdom SynCom was the direct cause of the dysbiotic phenotype in the cyp79b2/b3 mutant and 2) bacterial commensals and host tryptophan metabolism are both necessary to control fungal load, thereby promoting A. thaliana growth and survival. Our results indicate that protective activities of bacterial root commensals are as critical as the host tryptophan metabolic pathway in preventing fungal dysbiosis in the A. thaliana root endosphere
Analysis of cytosine methylation in genomic dna of solanum × michoacanum (+) s. tuberosum somatic hybrids
Interspecific somatic hybridization is a noteworthy breeding strategy that allows the production of novel genetic variability when crossing barriers exist between two parental species. Although the genetic consequences of somatic hybridization have been well documented, little is known on its impact at the epigenetic level. The objective of our research was to investigate the epigenetic changes, in particular DNA methylation, occurring in a population of potato somatic hybrids. The analysis of 96 Solanum × michoacanum (+) S. tuberosum somatic hybrids from five fusion combinations and their parents was carried out by methylation-sensitive amplified polymorphism (MSAP) and high-performance liquid chromatography (HPLC) methods. Six MSAP primer combinations generated 622 unique bands, of which 295 were fully methylated. HPLC analysis showed from 15.5% to 16.9% total cytosine methylation within the parental forms. Overall, the MSAP and HPLC methods indicated an increase in DNA methylation in the somatic hybrids in comparison to their parents. Among the latter, a lower degree of DNA methylation in the wild S. × michoacanum species than S. tuberosum was found. Our findings indicated that somatic hybridization changed the level of cytosine methylation in the studied potato somatic hybrids
Declination dependence of the cosmic-ray flux at extreme energies
We study the large-scale distribution of the arrival directions of the
highest energy cosmic rays observed by various experiments. Despite clearly
insufficient statistics, we find a deficit of cosmic rays at energies higher
than 10^{20} eV from a large part of the sky around the celestial North Pole.
We speculate on possible explanations of this feature.Comment: 5 pages, 4 figures; v2: 11 pages, 4 figures, title changed (to avoid
confusion with the Southern hemisphere), analysis extended, more data
included, results unchanged; to be published in JCA
Probing the potential landscape inside a two-dimensional electron-gas
We report direct observations of the scattering potentials in a
two-dimensional electron-gas using electron-beam diffaction-experiments. The
diffracting objects are local density-fluctuations caused by the spatial and
charge-state distribution of the donors in the GaAs-(Al,Ga)As heterostructures.
The scatterers can be manipulated externally by sample illumination, or by
cooling the sample down under depleted conditions.Comment: 4 pages, 4 figure
Gamma-rays from millisecond pulsars in Globular Clusters
Globular clusters (GCs) with their ages of the order of several billion years
contain many final products of evolution of stars such as: neutron stars, white
dwarfs and probably also black holes. These compact objects can be at present
responsible for the acceleration of particles to relativistic energies.
Therefore, gamma-ray emission is expected from GCs as a result of radiation
processes occurring either in the inner magnetosperes of millisecond pulsars or
in the vicinity of accreting neutron stars and white dwarfs or as a result of
interaction of particles leaving the compact objects with the strong radiation
field within the GC. Recently, GeV gamma-ray emission has been detected from
several GCs by the new satellite observatory Fermi. Also Cherenkov telescopes
reported interesting upper limits at the TeV energies which start to constrain
the content of GCs. We review the results of these gamma-ray observations in
the context of recent scenarios for their origin.Comment: 20 pages, 9 figures, will be published in Astrophysics and Space
Science Series (Springer), eds. N. Rea and D.F. Torre
Boundary spanning at the science–policy interface: the practitioners’ perspectives
Cultivating a more dynamic relationship between science and policy is essential for responding to complex social challenges such as sustainability. One approach to doing so is to “span the boundaries” between science and decision making and create a more comprehensive and inclusive knowledge exchange process. The exact definition and role of boundary spanning, however, can be nebulous. Indeed, boundary spanning often gets conflated and confused with other approaches to connecting science and policy, such as science communication, applied science, and advocacy, which can hinder progress in the field of boundary spanning. To help overcome this, in this perspective, we present the outcomes from a recent workshop of boundary-spanning practitioners gathered to (1) articulate a definition of what it means to work at this interface (“boundary spanning”) and the types of activities it encompasses; (2) present a value proposition of these efforts to build better relationships between science and policy; and (3) identify opportunities to more effectively mainstream boundary-spanning activities. Drawing on our collective experiences, we suggest that boundary spanning has the potential to increase the efficiency by which useful research is produced, foster the capacity to absorb new evidence and perspectives into sustainability decision-making, enhance research relevance for societal challenges, and open new policy windows. We provide examples from our work that illustrate this potential. By offering these propositions for the value of boundary spanning, we hope to encourage a more robust discussion of how to achieve evidence-informed decision-making for sustainability.Support
for the workshop was provided by Margaret A. Cargill Philanthropies
and The Pew Charitable Trusts. PFEA is supported by the U.K. Natural
Environment Research Council (NE/N005457/1)
High Energy Processes in Pulsar Wind Nebulae
Young pulsars produce relativistic winds which interact with matter ejected
during the supernova explosion and the surrounding interstellar gas. Particles
are accelerated to very high energies somewhere in the pulsar winds or at the
shocks produced in collisions of the winds with the surrounding medium. As a
result of interactions of relativistic leptons with the magnetic field and low
energy radiation (of synchrotron origin, thermal, or microwave background), the
non-thermal radiation is produced with the lowest possible energies up to
100 TeV. The high energy (TeV) gamma-ray emission has been originally
observed from the Crab Nebula and recently from several other objects. Recent
observations by the HESS Cherenkov telescopes allow to study for the first time
morphology of the sources of high energy emission, showing unexpected spectral
features. They might be also interpreted as due to acceleration of hadrons.
However, theory of particle acceleration in the PWNe and models for production
of radiation are still at their early stage of development since it becomes
clear that realistic modeling of these objects should include their time
evolution and three-dimensional geometry. In this paper we concentrate on the
attempts to create a model for the high energy processes inside the PWNe which
includes existence not only relativistic leptons but also hadrons inside the
nebula. Such model should also take into account evolution of the nebula in
time. Possible high energy expectations based on such a model are discussed in
the context of new observations.Comment: 9 pages, 1 figure, Proc. Multimessenger approach to high energy
gamma-ray source
Binaries with the eyes of CTA
The binary systems that have been detected in gamma rays have proven very
useful to study high-energy processes, in particular particle acceleration,
emission and radiation reprocessing, and the dynamics of the underlying
magnetized flows. Binary systems, either detected or potential gamma-ray
emitters, can be grouped in different subclasses depending on the nature of the
binary components or the origin of the particle acceleration: the interaction
of the winds of either a pulsar and a massive star or two massive stars;
accretion onto a compact object and jet formation; and interaction of a
relativistic outflow with the external medium. We evaluate the potentialities
of an instrument like the Cherenkov telescope array (CTA) to study the
non-thermal physics of gamma-ray binaries, which requires the observation of
high-energy phenomena at different time and spatial scales. We analyze the
capability of CTA, under different configurations, to probe the spectral,
temporal and spatial behavior of gamma-ray binaries in the context of the known
or expected physics of these sources. CTA will be able to probe with high
spectral, temporal and spatial resolution the physical processes behind the
gamma-ray emission in binaries, significantly increasing as well the number of
known sources. This will allow the derivation of information on the particle
acceleration and emission sites qualitatively better than what is currently
available.Comment: 23 pages, 13 figures, accepted for publication in Astroparticle
Physics, special issue on Physics with the Cherenkov Telescope Arra
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