A comparison of quantitative proteomics methodologies on a differential experiment on test samples

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Identificación de marcadores de superficie celular en célulasin iniciadoras del cáncer procedentes de pacientes con glioma

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Effect of autoimmune regulator protein (AIRE) expression of the cellular proteome

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Caracterización de nuevos sustratos de metaloproteasas en cáncer de mama mediante marcaje metabólico diferencial (SILAC)

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Cancer degradomics: ADAMI7 regulates TGF-BETA signaling through the cleavage of vasorin

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Evaluation of peptide alkylation side-resctions in common protein digestion protocols

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Exosomics - A Review Of Biophysics, Biology And Biochemistry Of Exosomes With A Focus On Human Breast Milk

Exosomes are biomolecular nanostructures released from cells. They carry specific biomolecular information and are mainly researched for their exquisite properties as a biomarker source and delivery system. We introduce exosomes in the context of other extracellular vesicles, describe their biophysical isolation and characterisation and discuss their biochemical profiling. Motivated by our interest in early-life nutrition and health, and corresponding studies enrolling lactating mothers and their infants, we zoom into exosomes derived from human breast milk. We argue that these should be more extensively studied at proteomic and micronutrient profiling level, because breast milk exosomes provide a more specific window into breast milk quality from an immunological (proteomics) and nutritional (micronutrient) perspective. Such enhanced breast milk exosome profiling would thereby complement and enrich the more classical whole breast milk analysis and is expected to deliver more functional insights than the rather descriptive analysis of human milk, or larger fractions thereof, such as milk fat globule membrane. We substantiate our arguments by a bioinformatic analysis of two published proteomic data sets of human breast milk exosomes

Expresión proteica diferencial de tratamiento de fotocoagulación con láser en células ARPE

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A proteored multi-centric study to assess reproducibility of relative quantification by LC-MS proteomic analysis

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Eficacia de las Intervenciones de Terapia Psicomotriz en ansiedad y estrés laboral en el personal de salud

This study analyzes the improvements in anxiety and work stress of a group of health workers from the Faculty of Medicine of the University of the Republic (Uruguay). The sample amounts to 133 people to whom various types of tests are applied to measure and evaluate their situation in the aforementioned aspects. The evaluation of the efficacy of therapeutic psychomotor interventions is carried out from standardized tests and to be standardized, for the population in question. Four validated tests are used: Maslach Burnout Inventory for Human Services - MBI-HSS (Maslach, Jackson, Leiter, Schaufeli & Schwab, 1986), Copenhagen Psychosocial Questionnaire - COPSOQ III ((Burr et al., 2019), Multidimensional Assessment of Consciousness Body Interoceptive - Multidimensional Assessment of Interoceptive Awareness - MAIA, 12 sub scales self-regulation and thrust (Mehling et al., 2012) and General Health Questionnaire - G.H.Q.-28 items (Goldberg, 1972).They are applied before the start of the therapeutic psychomotor interventions, and at the end of the 12-session cycle. A psychomotor intervention program is developed as an effective tool for improving personal anxiety and stress conditions caused by complex situations in the workplace.El presente estudio analiza las mejoras en ansiedad y estrés laboral de un grupo de trabajadoras y trabajadores de la salud de la Facultad de Medicina de la Universidad de la República (Uruguay). La muestra asciende a 133 personas a quienes se les aplican varios tipos de test para medir y evaluar su situación en los aspectos mencionados. La evaluación de la eficacia de las intervenciones psicomotrices terapéuticas se realiza a partir de pruebas estandarizadas y a estandarizar, para la población en cuestión. Se utilizan cuatro test validados: Maslach Burnout Inventory for Human Services - MBI-HSS (Maslach, Jackson, Leiter, Schaufeli y Schwab, 1986), Copenhagen Psychosocial Questionnaire - COPSOQ III ((Burr et al., 2019), Evaluación Multidimensional de Conciencia Corporal Interoceptiva - Multidimensional Assessment of Interoceptive Awareness - MAIA, 12 sub scales self-regulation and thrust (Mehling et al., 2012) y General Health Questionnaire - G.H.Q.-28 items (Goldberg, 1972). Son aplicados antes del comienzo de las intervenciones psicomotrices terapéuticas, y al finalizar el ciclo de 12 sesiones. Se desarrolla un programa de intervención psicomotriz como una herramienta eficaz para la mejora de las condiciones personales de ansiedad y estrés provocados por situaciones complejas en el ámbito laboral