B-Cell and Monocyte Contribution to Systemic Lupus Erythematosus Identified by Cell-Type-Specific Differential Expression Analysis in RNA-Seq Data

Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by complex interplay among immune cell types. SLE activity is experimentally assessed by several blood tests, including gene expression profiling of heterogeneous populations of cells in peripheral blood. To better understand the contribution of different cell types in SLE pathogenesis, we applied the two methods in cell-type-specific differential expression analysis, csSAM and DSection, to identify cell-type-specific gene expression differences in heterogeneous gene expression measures obtained using RNA-seq technology. We identified B-cell-, monocyte-, and neutrophil-specific gene expression differences. Immunoglobulin-coding gene expression was altered in B-cells, while a ribosomal signature was prominent in monocytes. On the contrary, genes differentially expressed in the heterogeneous mixture of cells did not show any functional enrichment. Our results identify antigen binding and structural constituents of ribosomes as functions altered by B-cell- and monocyte-specific gene expression differences, respectively. Finally, these results position both csSAM and DSection methods as viable techniques for celltype-specific differential expression analysis, which may help uncover pathogenic, cell-type-specific processes in SLE

Keys of a Mission to Uranus or Neptune, the Closest Ice Giants

Uranus and Neptune are the archetypes of "ice giants", a class of planets that may be among the most common in the Galaxy. They are the last unexplored planets of the Solar System, yet they hold the keys to understand the atmospheric dynamics and structure of planets with hydrogen atmospheres inside and outside the solar system

Eating disorders in weight-related therapy (EDIT): protocol for a systematic review with individual participant data meta-analysis of eating disorder risk in behavioural weight management

The Eating Disorders In weight-related Therapy (EDIT) Collaboration brings together data from randomised controlled trials of behavioural weight management interventions to identify individual participant risk factors and intervention strategies that contribute to eating disorder risk. We present a protocol for a systematic review and individual participant data (IPD) meta-analysis which aims to identify participants at risk of developing eating disorders, or related symptoms, during or after weight management interventions conducted in adolescents or adults with overweight or obesity. We systematically searched four databases up to March 2022 and clinical trials registries to May 2022 to identify randomised controlled trials of weight management interventions conducted in adolescents or adults with overweight or obesity that measured eating disorder risk at pre- and post-intervention or follow-up. Authors from eligible trials have been invited to share their deidentified IPD. Two IPD meta-analyses will be conducted. The first IPD meta-analysis aims to examine participant level factors associated with a change in eating disorder scores during and following a weight management intervention. To do this we will examine baseline variables that predict change in eating disorder risk within intervention arms. The second IPD meta-analysis aims to assess whether there are participant level factors that predict whether participation in an intervention is more or less likely than no intervention to lead to a change in eating disorder risk. To do this, we will examine if there are differences in predictors of eating disorder risk between intervention and no-treatment control arms. The primary outcome will be a standardised mean difference in global eating disorder score from baseline to immediately post-intervention and at 6- and 12- months follow-up. Identifying participant level risk factors predicting eating disorder risk will inform screening and monitoring protocols to allow early identification and intervention for those at risk

Connections & Edges: 2040 Lincoln District Vision Plan

The Lincoln Vision Plan presents a concept for the evolution of Portland’s south downtown into a place where state-of-the-art resource management practices, unique systems of public open spaces, and a variety of transit options will support a vibrant, dynamic, high density, ecologically-friendly neighborhood. It is our hope that this Plan, created by Portland State University’s spring 2008 Urban Design Workshop, can serve as a foundation for further discussion and action to bring to life the immense potential underlying this district. This project was conducted under the supervision of Donald J. Stastny and Edward Starkie

Keys of a Mission to Uranus or Neptune, the Closest Ice Giants

A White Paper for the NASA Decadal Survey of Planetary Sciences and AstrobiologyUranus and Neptune are the archetypes of "ice giants", a class of planets that may be among the most common in the Galaxy. They hold the keys to understand the atmospheric dynamics and structure of planets with hydrogen atmospheres inside and outside the solar system; however, they are also the last unexplored planets of the Solar System. Their atmospheres are active and storms are believed to be fueled by methane condensation which is both extremely abundant and occurs at low optical depth. This means that mapping temperature and methane abundance as a function of position and depth will inform us on how convection organizes in an atmosphere with no surface and condensates that are heavier than the surrounding air, a general feature of giant planets. Owing to the spatial and temporal variability of these atmospheres, an orbiter is required. A probe would provide a reference atmospheric profile to lift ambiguities inherent to remote observations. It would also measure the abundances of noble gases which can be used to reconstruct the history of planet formation in the Solar System. Finally, mapping the planets' gravity and magnetic fields will be essential to constrain their global composition, atmospheric dynamics, structure and evolution. An exploration of Uranus or Neptune will be essential to understand these planets and will also be key to constrain and analyze data obtained at Jupiter, Saturn, and for numerous exoplanets with hydrogen atmospheres

College, Jobs, or the Military? Enlistment During a Time of War

This article questions what factors are associated with joining the military after high school rather than attending college, joining the civilian labor force, or doing some other activity. Three areas of influence on military enlistment are highlighted: educational goals, the institutional presence of the military in communities, and race and socioeconomic status. Copyright (c) 2006 Southwestern Social Science Association.