The Vehicle, Fall 1988

Table of Contents Cover LetterBob Zordanipage 3 Letter to Harrington StreetBob Zordanipage 4 The Only TruthBob Zordanipage 5 They\u27d Gone to a MovieMatt Mansfieldpage 6 The LocketMonica Grothpage 6 The Sleep of BabesMonica Grothpage 7 Techni-Color Characters in a Black and White TownMonica Grothpage 8 The HorseRodger Patiencepage 9 ValaciaRobyn Kerrpage 10 Gatsby\u27s LightJim Reedpage 11 Millions of MeJim Reedpage 12 View from the StreetsSteven M. Beamerpage 13 When Headlights on the HighwayMichael Salempage 23 Concrete AffairsMichael Salempage 24 The Middle of the StreetMichael Salempage 25 Scent of a StormMichael Salempage 26 The FishermanAngie Geraldpage 27 OrgansPatrick Peterspage 33 CarpentryPatrick Peterspage 34 FishingPatrick Peterspage 35 Autumn Poem for a Friend In a Printing PlantPatrick Peterspage 36https://thekeep.eiu.edu/vehicle/1051/thumbnail.jp

The Vehicle, Fall 1988

Table of Contents Cover LetterBob Zordanipage 3 Letter to Harrington StreetBob Zordanipage 4 The Only TruthBob Zordanipage 5 They\u27d Gone to a MovieMatt Mansfieldpage 6 The LocketMonica Grothpage 6 The Sleep of BabesMonica Grothpage 7 Techni-Color Characters in a Black and White TownMonica Grothpage 8 The HorseRodger Patiencepage 9 ValaciaRobyn Kerrpage 10 Gatsby\u27s LightJim Reedpage 11 Millions of MeJim Reedpage 12 View from the StreetsSteven M. Beamerpage 13 When Headlights on the HighwayMichael Salempage 23 Concrete AffairsMichael Salempage 24 The Middle of the StreetMichael Salempage 25 Scent of a StormMichael Salempage 26 The FishermanAngie Geraldpage 27 OrgansPatrick Peterspage 33 CarpentryPatrick Peterspage 34 FishingPatrick Peterspage 35 Autumn Poem for a Friend In a Printing PlantPatrick Peterspage 36https://thekeep.eiu.edu/vehicle/1051/thumbnail.jp

The Vehicle, Fall 1990

Table of Contents Poetry Loss of the SpokenMatt Mansfieldpage 6-7 A Wyeth ReproductionLynn A. Rafoolpage 8 CornerSuzanna Portpage 9 FatherSuzanna Portpage 10 Past two,Victoria Bennettpage 11 VertigoVictoria Bennettpage 12 HeatVictoria Bennettpage 13 Pre-HistoryLuiz da Silveirapage 14 BloomsLuiz da Silveirapage 15 Hashimoto\u27s Thyrotoxicosis at Key WestAnthony Smithpage 16 Davy by the Pawn ShopAnthony Smithpage 17 Art UntitledMark Randallpage 19 UntitledMark Randallpage 20 UntitledSteve Reyespage 21 UntitledSteve Reyespage 22 Photographs UntitledSteve Beamerpage 24-25 UntitledSteve Beamerpage 26 UntitledSteve Beamerpage 27 Prose YikesMichael Brownpage 29 Thirty Minutes or LessSteve Fitzgeraldpage 30-35 Telling StoriesMatt Mansfieldpage 36-40 Interview Poet Donald HallMatt Mansfieldpage 42-44 Authors Biographiespage 46-47https://thekeep.eiu.edu/vehicle/1054/thumbnail.jp

The Vehicle, Spring 1988

Contents CitizenCraig Titleypage 3 Justice DistributionAngie Geraldpage 4 Letter from My MotherDan Von Holtenpage 6 The Fool and His LadyRodger Patiencepage 7 What Big IsBob Zordanipage 8 Blind ArchitectureJim Reedpage 9 Ah, What a Funny Thing These EyesCraig Titleypage 10 FarmingPatrick Peterspage 11 The CowSteven M. Beamerpage 13 The Enigma of My ExistenceAngie Kathpage 14 Neon TeethMichael Salempage 15 Zombie PoseidonMichael Salempage 16 From: Letters from the Linder BuildingPatrick Peters, Bob Zordani, Dan Von Holtenpage 17 A Man of Jackson IV (City Dweller)Steven M. Beamerpage 19 11:45 p.m. FridayElizabeth McMeekanpage 20 Mr. Siegel-January 1967Timothy J. McCarthypage 21 She had a look about herA bag man of Jackson pauses for a portraitSteven M. Beamerpage 22 Citybed (Homeless of Chicago)Steven M. Beamerpage 23 Electra and the Eighteen WheelerTammy Veachpage 24 OrderRhonda Ealypage 28 Grave Mill ISteven M. Beamerpage 29 The Party (A Social Event in 80 Words or Less)Groucho Smithpage 30 Graceland and Elvis Presley EarmuffsCraig Titleypage 31 Learning to ForageDan Von Holtenpage 33 One Last Peanut at Gateway CenterReflections of Three with DeCicco and LadySteven M. Beamerpage 34 Letter from the MidwestPatrick Peterspage 35 WoodwindJim Reedpage 36 Excerpts from Notebook AustraliaKeith Schusterpage 37 Letter from the DesertPatrick Peterspage 40 Tinted Blue, Waiting for an Uptown TaxiMichael Salempage 42 Voice and Earth: A Review of The Invention of the Telephone by Bruce GuernseyDan Von Holtenpage 43https://thekeep.eiu.edu/vehicle/1052/thumbnail.jp

The Vehicle, Spring 1988

Contents CitizenCraig Titleypage 3 Justice DistributionAngie Geraldpage 4 Letter from My MotherDan Von Holtenpage 6 The Fool and His LadyRodger Patiencepage 7 What Big IsBob Zordanipage 8 Blind ArchitectureJim Reedpage 9 Ah, What a Funny Thing These EyesCraig Titleypage 10 FarmingPatrick Peterspage 11 The CowSteven M. Beamerpage 13 The Enigma of My ExistenceAngie Kathpage 14 Neon TeethMichael Salempage 15 Zombie PoseidonMichael Salempage 16 From: Letters from the Linder BuildingPatrick Peters, Bob Zordani, Dan Von Holtenpage 17 A Man of Jackson IV (City Dweller)Steven M. Beamerpage 19 11:45 p.m. FridayElizabeth McMeekanpage 20 Mr. Siegel-January 1967Timothy J. McCarthypage 21 She had a look about herA bag man of Jackson pauses for a portraitSteven M. Beamerpage 22 Citybed (Homeless of Chicago)Steven M. Beamerpage 23 Electra and the Eighteen WheelerTammy Veachpage 24 OrderRhonda Ealypage 28 Grave Mill ISteven M. Beamerpage 29 The Party (A Social Event in 80 Words or Less)Groucho Smithpage 30 Graceland and Elvis Presley EarmuffsCraig Titleypage 31 Learning to ForageDan Von Holtenpage 33 One Last Peanut at Gateway CenterReflections of Three with DeCicco and LadySteven M. Beamerpage 34 Letter from the MidwestPatrick Peterspage 35 WoodwindJim Reedpage 36 Excerpts from Notebook AustraliaKeith Schusterpage 37 Letter from the DesertPatrick Peterspage 40 Tinted Blue, Waiting for an Uptown TaxiMichael Salempage 42 Voice and Earth: A Review of The Invention of the Telephone by Bruce GuernseyDan Von Holtenpage 43https://thekeep.eiu.edu/vehicle/1052/thumbnail.jp

The Vehicle, Fall 1987

Table of Contents Sketches in the SunRodger L. Patiencepage 3 Reflecting PoolRob Montgomerypage 5 Grandpa\u27s Porcelain DollRichard E. Hallpage 6 Tintype 1837Catherine Friemannpage 6 PhotographSteven M. Beamerpage 7 Washerwoman\u27s SongBob Zordanipage 8 Scrambled Eggs for D.O.Lynne A. Rafoolpage 8 my mother would sayMonica Grothpage 9 Retired by His ChildrenDan Von Holtenpage 10 I am the oldestMonica Grothpage 11 Ice on WheatRob Montgomerypage 12 The Nature of the RoseTroy Mayfieldpage 12 Past NebraskaDan Hornbostelpage 13 Five Minute Jamaican VacationChristy Dunphypage 14 PhotographSteven M. Beamerpage 14 The Angry PoemChristy Dunphypage 15 Road UnfamiliarChristy Dunphypage 15 raised voicesMonica Grothpage 16 Old Ladies & MiniskirtsKara Shannonpage 17 FreakspeakBob Zordanipage 18 PortraitDan Von Holtenpage 18 Mobile VacuumKathleen L. Fairfieldpage 19 Rev. Fermus DickSteve Hagemannpage 20 PhotographSteven M. Beamerpage 21 What\u27s the Name of That Flower?Richard Jesse Davispage 22 RequestChristy Dunphypage 23 SketchPaul Seabaughpage 24 ExperiencedMarilyn Wilsonpage 26 Leaving: Two ViewsTina Phillipspage 27 AntaeusDan Von Holtenpage 28 Misogyny at 19J. D. Finfrockpage 29 A Mental CrippleSteve Hagemannpage 32 AssociationsRhonda Ealypage 33 Banana BreadGail Bowerpage 34 Bill and JackBradford B. Autenpage 35 After Image No. 2Rob Montgomerypage 35 VrrooomBeth Goodmanpage 36 Mr. Modern LoverMolly Maddenpage 36 TravelogueRodger L. Patiencepage 37 Down the HighwayJoan Sebastianpage 38 A Retread HeavenRob Montgomerypage 41 StuporDan Von Holtenpage 42 Love Poem After a Seizure in Your BedBob Zordanipage 43 PalsyChristy Dunphypage 44 Interview with Mr. MatthewsBob Zordanipage 45 Chasing Down Hot Air Balloons on a Sunday MorningRob Montgomerypage 48https://thekeep.eiu.edu/vehicle/1049/thumbnail.jp

Genetic randomization reveals functional relationships among morphologic and tissue-quality traits that contribute to bone strength and fragility

We examined femora from adult AXB/BXA recombinant inbred (RI) mouse strains to identify skeletal traits that are functionally related and to determine how functional interactions among these traits contribute to genetic variability in whole-bone stiffness, strength, and toughness. Randomization of A/J and C57BL/6J genomic regions resulted in each adult male and female RI strain building mechanically functional femora by assembling unique sets of morphologic and tissue-quality traits. A correlation analysis was conducted using the mean trait values for each RI strain. A third of the 66 correlations examined were significant, indicating that many bone traits covaried or were functionally related. Path analysis revealed important functional interactions among bone slenderness, cortical thickness, and tissue mineral density. The path coefficients describing these functional relations were similar for both sexes. The causal relationship among these three traits suggested that cellular processes during growth simultaneously regulate bone slenderness, cortical thickness, and tissue mineral density so that the combination of traits is sufficiently stiff and strong to satisfy daily loading demands. A disadvantage of these functional interactions was that increases in tissue mineral density also deleteriously affected tissue ductility. Consequently, slender bones with high mineral density may be stiff and strong but they are also brittle. Thus, genetically randomized mouse strains revealed a basic biological paradigm that allows for flexibility in building bones that are functional for daily activities but that creates preferred sets of traits under extreme loading conditions. Genetic or environmental perturbations that alter these functional interactions during growth would be expected to lead to loss of function and suboptimal adult bone quality

Combination of cytokine responses indicative of latent TB and active TB in Malawian adults.

BACKGROUND: An IFN-γ response to M. tuberculosis-specific antigens is an effective biomarker for M. tuberculosis infection but it cannot discriminate between latent TB infection and active TB disease. Combining a number of cytokine/chemokine responses to M. tuberculosis antigens may enable differentiation of latent TB from active disease. METHODS: Asymptomatic recently-exposed individuals (spouses of TB patients) were recruited and tuberculin skin tested, bled and followed-up for two years. Culture supernatants, from a six-day culture of diluted whole blood samples stimulated with M. tuberculosis-derived PPD or ESAT-6, were measured for IFN-γ, IL-10, IL-13, IL-17, TNF-α and CXCL10 using cytokine ELISAs. In addition, 15 patients with sputum smear-positive pulmonary TB were recruited and tested. RESULTS: Spouses with positive IFN-γ responses to M. tuberculosis ESAT-6 (>62.5 pg/mL) and TB patients showed high production of IL-17, CXCL10 and TNF-α. Higher production of IL-10 and IL-17 in response to ESAT-6 was observed in the spouses compared with TB patients while the ratios of IFN-γ/IL-10 and IFN-γ/IL-17 in response to M. tuberculosis-derived PPD were significantly higher in TB patients compared with the spouses. Tuberculin skin test results did not correlate with cytokine responses. CONCLUSIONS: CXCL10 and TNF-α may be used as adjunct markers alongside an IFN-γ release assay to diagnose M. tuberculosis infection, and IL-17 and IL-10 production may differentiate individuals with LTBI from active TB

Phosphorylation-Dependent Effects on the Structural Flexibility of Phosphoglucosamine Mutase from Bacillus anthracis

Phosphoglucosamine mutase (PNGM) is an evolutionarily conserved bacterial enzyme in the peptidoglycan biosynthetic pathway, catalyzing the reversible conversion between glucosamine 1- and 6-phosphate. Previous structural studies of PNGM from the pathogen Bacillus anthracis revealed its dimeric assembly and highlighted the rotational mobility of its C-terminal domain. Recent studies of two other enzymes in the same superfamily have demonstrated the long-range effects on the conformational flexibility associated with phosphorylation of the conserved, active site phosphoserine involved in phosphoryl transfer. Building on this work, we use a combination of experimental and computational studies to show that the active, phosphorylated version of B. anthracis PNGM has decreased flexibility relative to its inactive, dephosphorylated state. Limited proteolysis reveals an enhanced and accelerated cleavage of the dephosphorylated enzyme. ¹⁵N transverse relaxation-optimized NMR spectra corroborate a conformational adjustment with broadening and shifts of peaks relative to the phospho-enzyme. Electrostatic calculations indicate that residues in the mobile, C-terminal domain are linked to the phosphoserine by lines of attraction that are absent in the dephosphorylated enzyme. Phosphorylation-dependent changes in protein flexibility appear linked with the conformational change and enzyme mechanism in PNGM, establishing this as a conserved theme in multiple subgroups of the diverse α-d-phosphohexomutase superfamily

Phosphorylation-Dependent Effects on the Structural Flexibility of Phosphoglucosamine Mutase from Bacillus anthracis

Phosphoglucosamine mutase (PNGM) is an evolutionarily conserved bacterial enzyme in the peptidoglycan biosynthetic pathway, catalyzing the reversible conversion between glucosamine 1- and 6-phosphate. Previous structural studies of PNGM from the pathogen Bacillus anthracis revealed its dimeric assembly and highlighted the rotational mobility of its C-terminal domain. Recent studies of two other enzymes in the same superfamily have demonstrated the long-range effects on the conformational flexibility associated with phosphorylation of the conserved, active site phosphoserine involved in phosphoryl transfer. Building on this work, we use a combination of experimental and computational studies to show that the active, phosphorylated version of B. anthracis PNGM has decreased flexibility relative to its inactive, dephosphorylated state. Limited proteolysis reveals an enhanced and accelerated cleavage of the dephosphorylated enzyme. ¹⁵N transverse relaxation-optimized NMR spectra corroborate a conformational adjustment with broadening and shifts of peaks relative to the phospho-enzyme. Electrostatic calculations indicate that residues in the mobile, C-terminal domain are linked to the phosphoserine by lines of attraction that are absent in the dephosphorylated enzyme. Phosphorylation-dependent changes in protein flexibility appear linked with the conformational change and enzyme mechanism in PNGM, establishing this as a conserved theme in multiple subgroups of the diverse α-d-phosphohexomutase superfamily