Chromosomal evolution in allopatric populations of the <i>Odontophrynus occidentalis</i> group (Amphibia, Anura) from western Argentina

A karyological study of some populations of the Odontophrynus occidentalis group (2n=22) from Argentina is reported. Ammoniacal silver (Ag-AS) and alkaline Giemsa (CBG) staining have been performed on specimens from La Rioja, Mendoza, Malargue, Tamelen, Tapiluque, Valcheta, and Meseta de Somuncura. All populations showed active ribosomal cistrons (NORs) on chromosome 11. Some metaphases of three specimens from Mendoza and five from La Rioja also showed bands on 9. Centromeric and telomeric C-bands were common to all populations, and a block of heterochromatin on both sides of the centromere of 2 was remarkable in all but the La Rioja populations. The specimens from La Rioja showed a similar block of heterochromatin on the short arm of 1. Taxonomic studies of several specimens from these localities revealed differences in larval development, glandular pattern, the size of specimens, etc. As a whole, biological as well as karyological features suggest that the isolated population at La Rioja is a separate entity, at the species level, as described elsewhere.Material digitalizado en SEDICI gracias a la colaboración del Dr. Jorge Williams (FCNM-UNLP).Facultad de Ciencias Naturales y Muse

Chromosomal evolution in allopatric populations of the <i>Odontophrynus occidentalis</i> group (Amphibia, Anura) from western Argentina

A karyological study of some populations of the Odontophrynus occidentalis group (2n=22) from Argentina is reported. Ammoniacal silver (Ag-AS) and alkaline Giemsa (CBG) staining have been performed on specimens from La Rioja, Mendoza, Malargue, Tamelen, Tapiluque, Valcheta, and Meseta de Somuncura. All populations showed active ribosomal cistrons (NORs) on chromosome 11. Some metaphases of three specimens from Mendoza and five from La Rioja also showed bands on 9. Centromeric and telomeric C-bands were common to all populations, and a block of heterochromatin on both sides of the centromere of 2 was remarkable in all but the La Rioja populations. The specimens from La Rioja showed a similar block of heterochromatin on the short arm of 1. Taxonomic studies of several specimens from these localities revealed differences in larval development, glandular pattern, the size of specimens, etc. As a whole, biological as well as karyological features suggest that the isolated population at La Rioja is a separate entity, at the species level, as described elsewhere.Material digitalizado en SEDICI gracias a la colaboración del Dr. Jorge Williams (FCNM-UNLP).Facultad de Ciencias Naturales y Muse

Chromosomal evolution in allopatric populations of the <i>Odontophrynus occidentalis</i> group (Amphibia, Anura) from western Argentina

A karyological study of some populations of the Odontophrynus occidentalis group (2n=22) from Argentina is reported. Ammoniacal silver (Ag-AS) and alkaline Giemsa (CBG) staining have been performed on specimens from La Rioja, Mendoza, Malargue, Tamelen, Tapiluque, Valcheta, and Meseta de Somuncura. All populations showed active ribosomal cistrons (NORs) on chromosome 11. Some metaphases of three specimens from Mendoza and five from La Rioja also showed bands on 9. Centromeric and telomeric C-bands were common to all populations, and a block of heterochromatin on both sides of the centromere of 2 was remarkable in all but the La Rioja populations. The specimens from La Rioja showed a similar block of heterochromatin on the short arm of 1. Taxonomic studies of several specimens from these localities revealed differences in larval development, glandular pattern, the size of specimens, etc. As a whole, biological as well as karyological features suggest that the isolated population at La Rioja is a separate entity, at the species level, as described elsewhere.Material digitalizado en SEDICI gracias a la colaboración del Dr. Jorge Williams (FCNM-UNLP).Facultad de Ciencias Naturales y Muse

Bovine Papillomavirus Clastogenic Effect Analyzed in Comet Assay

Bovine papillomavirus (BPV) is an oncogenic virus related to serious livestock diseases. Oncoproteins encoded by BPV are involved in several steps of cellular transformation and have been reported as presenting clastogenic effects in peripheral lymphocytes and primary culture cells. the aim of this study was to evaluate the clastogenic potential of BPV types 1, 2, and 4 by comet assay. Peripheral blood was collected from 37 bovines, 32 infected with different levels of papillomatosis (12 animals have no affection) and five calves, virus free (negative control). the viral identification showed presence of more than one virus type in 59.375% of the infected animals. Comet assay was performed according to alkaline technique. the Kruskal-Wallis test showed statistical difference between the negative control group and infected animals (P = 0.0015). the Dunn post hoc test showed difference comparing the infected animals with calves. Mann-Whitney U test verified no difference between animals infected with only one viral type and animals presenting more than one viral type. the comet assay is considered an efficient tool for assessment of damage in the host chromatin due to viral action, specifically highlighting viral activity in blood cells.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundacao do Desenvolvimento Administrativo (PAP-FUNDAP)Inst Butantan, Genet Lab, BR-05503900 São Paulo, BrazilUniv São Paulo, Inst Ciencias Biomed, Programa Posgrad Interunidades Biotecnol, BR-05508900 São Paulo, BrazilUniversidade Federal de São Paulo, Programa Posgrad Biol Estrutural & Func, BR-04023900 São Paulo, BrazilUniv Fed Integracao Latinoamer UNILA, Dept Biol, BR-85867970 Foz Do Iguacu, PR, BrazilUniversidade Federal de São Paulo, Programa Posgrad Biol Estrutural & Func, BR-04023900 São Paulo, BrazilCNPq: 402539/2011-7Web of Scienc

Persistence or Clearance of Human Papillomavirus Infections in Women in Ouro Preto, Brazil

Persistent high-risk (HR) human papillomavirus (HPV) infection is necessary for development of precursor lesions and cervical cancer. We investigate persistence and clearance of HPV infections and cofactors in unvaccinated women. Cervical samples of 569 women (18-75 years), received for routine evaluation in the Health Department of Ouro Preto, Brazil, were collected and subjected to PCR (MY09/11 orGP5+/6+ primers), followed by RFLP or sequencing. All women were interviewed to collect sociodemographic and behavioral information. Viral infection persistence or clearance was reevaluated after 24 months and was observed in 59.6% and 40.4% of women, respectively. HPVs 16, 33, 59, 66, 69, and 83 (HR) were the most persistent types whereas HPVs 31, 45, and 58 were less persistent. Clearance or persistence did not differ between groups infected by HPVs 18, 53, and 67. in low-risk (LR) types, HPV 6 infected samples were associated with clearance, while HPV 11, 61, 72, or 81 infected samples were persistent in the follow-up. No statistically significant association was detected between persistent HPV infections and sociodemographic and behavioral characteristics analyzed. To study persistence or clearance in HPV infection allows the identification of risk groups, cofactors, and strategies for prevention of cervical cancer.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)PRONEX/FACEPEFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Fundação ButantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Inst Butantan, Genet Lab, BR-05503900 São Paulo, BrazilUniv Ouro Preto UFOP, Escola Farm, Dept Anal Clin, BR-35400000 Ouro Preto, MG, BrazilUniversidade Federal de São Paulo, Dept Ginecol, Lab Tocoginecol, BR-04039032 São Paulo, BrazilUniv Fed Pernambuco UFPE, LIKA, BR-50670901 Recife, PE, BrazilUniv São Paulo, Programa Posgrad Interunidades Biotecnol, BR-05508900 São Paulo, BrazilUniv Fed Integracao Latinoamer UNILA, Dept Biol, BR-85867970 Foz Do Iguacu, PR, BrazilUniversidade Federal de São Paulo, Dept Ginecol, Lab Tocoginecol, BR-04039032 São Paulo, BrazilCNPq: 554816/2006-7PRONEX/FACEPE: APQ-0781-4.01/06FAPESP: 2006/02439-6Web of Scienc

Phylogenetic classification and clinical aspects of a new putative Deltapapillomavirus associated with skin lesions in cattle

Bovine papillomaviruses (BPVs) are recognized as causal agents of benign and malignant tumors in cattle. Thirteen types of BPVs have already been described and classified into 3 distinct genera. Divergences in the nucleotide sequence of the L1 gene are used to identify new viral types through the employment of PCR assays with degenerated primers. in the present study, a method for identifying BPVs based on PCR-RFLP and DNA sequencing allowed the identification of a new putative Deltapapillomavirus, designated JN/3SP (JQ280500.1). the analysis of the L1 gene showed that this strain was most closely related to the BPVs -1, -2, -13, and OaPV1 (71-73% genetic similarity). in this study, we describe the detection of this new putative Deltapapillomavirus type and verify its phylogenetic position within the genus.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Inst Butantan, Lab Genet, São Paulo, BrazilUniversidade Federal de São Paulo, Programa Posgrad Biol Estrutural & Func, São Paulo, BrazilUniv São Paulo, Programa Posgrad Interunidades Biotecnol, São Paulo, BrazilUniv Fed Pernambuco, Dept Bot, Recife, PE, BrazilUniv Fed Integracao Latino Amer, Dept Biol, Foz Do Iguacu, PR, BrazilUniversidade Federal de São Paulo, Programa Posgrad Biol Estrutural & Func, São Paulo, BrazilCNPq: 402539/2011-7CNPq: 554816/2006-7Web of Scienc

Human papillomavirus infection in Brazilian women with normal cervical cytology

We examined the prevalence of human papillomavirus (HPV) infection in a sample of Brazilian women presenting normal cervical cytology. Possible interactions between patient characteristics and HPV infection were analyzed in order to provide background data to improve cervical cancer screening and prophylaxis. Cervical samples of 399 women, received for routine evaluation in the Health Department of Ouro Preto, MG, Brazil, were subjected to HPV-DNA testing by PCR with MY09/11 primers. HPV-positive specimens were typed by RFLP. A structured epidemiological questionnaire was administered to each woman. HPV prevalence among these cytologically normal women was 11%. Twelve viral types were detected, the most common being HPV-16, -6, -61, -83, and -66. HPV was more prevalent in younger women; high-risk viral types were detected in 61% of the infected women and 27% of the infected women had multiple HPV infections. Significant associations of HPV infection were found with age, literacy, residence, marital status, lifetime number of sexual partners, and parity. We detected a great diversity of HPV types in women with normal cytology. This kind of information about local populations is useful for HPV prevention and vaccination strategies.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FACEPE (PRONEX)Inst Butantan, Dept Genet, Genet Lab, São Paulo, BrazilUniv Fed Ouro Preto, Escola Farm, Dept Anal Clin, Ouro Preto, MG, BrazilUniversidade Federal de São Paulo, Dept Ginecol, Lab Tocoginecol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Morfol & Genet, São Paulo, BrazilUniv Fed Pernambuco, Dept Bioquim, Lab Imunopatol Keizo Asami, Recife, PE, BrazilUniv Fed Integracao Latinoamer, Dept Ciencias Biol, Foz Do Iguacu, PR, BrazilUniversidade Federal de São Paulo, Dept Ginecol, Lab Tocoginecol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Morfol & Genet, São Paulo, BrazilWeb of Scienc