Histološka i imunohistokemijska svojstva metastatskog karcinosarkoma mliječne žlijezde u kuje - prikaz slučaja

A carcinosarcoma is a tumor composed of cells morphologically resembling both malignant epithelial and mesenchymal components. An 11-year-old, female, mixed-breed terrier was referred to the clinic with complaints of anorexia, vomiting and exercise intolerance. Clinically, tumoral masses were seen in the mammary glands. During necropsy, multilobulated calcified masses were observed in several visceral organs (the liver, kidneys, lungs, small intestines, lymph nodes and thyroid) and both right and left (3rd and 4th) mammary glands. Immunohistochemically, Thyroglobulin, SMA, TTF-1, S100, CD34, and p53 were negative in both epithelial and mesenchymal areas, however, Ki-67 showed high proliferation, especially in the mesenchymal areas. While the positivity of vimentin expression was high in the mesenchymal component of the tumor, the positivity of cytokeratin (CK7 and CK19) expressions was high in the epithelial component of the tumor. In the case of a carcinosarcoma, there is a probability that not only the carcinomatous part, but also the sarcomatous part will metastasize. Despite being uncommon, canine carcinosarcomas should always be considered in the differential diagnosis of mixed mammary tumors, if they include both components. There have not been many previous studies dealing with the metastasis of a mammary carcinosarcoma in dogs as an aggressive tumor. Therefore, we consider this report a worthy contribution and have defined the multiple organ metastasis of a mammary carcinosarcoma.Karcinosarkom je tumor sastavljen od stanica koje morfološki nalikuju i na maligne epitelne i na mezenhimne komponente. Ženka stara 11 godina, mješanka u tipu terijera, primljena je na pregled zbog anoreksije, povraćanja i nepodnošenja tjelesnog napora. Klinički, uočena je tumorska masa u mliječnim žlijezdama. Pri obdukciji su uočene multilobulirane kalcificirane tvrobe u nekoliko visceralnih organa (jetra, bubreg, pluća, tanko crijevo, limfni čvorovi, štitnjača) te u desnoj i lijevoj (trećoj i četvrtoj) mliječnoj žlijezdi. Imunohistokemijski su tireoglobulin, SMA, TTF-1, S100, CD34 i p53 bili negativni i u epitelnim i mezenhimnim tkivima, međutim Ki-67 pokazao je visoku proliferaciju, osobito u mezenhimnom području. Dok je pozitivnost ekspresije vimentina bila visoka u mezenhimnoj komponenti tumora, pozitivnost ekspresije citokeratina (CK7 i CK19) bila je visoka u epitelnoj komponenti tumora. U slučaju karcinosarkoma moguće je da ne metastaziraju samo karcinomatozni dijelovi nego također i sarkomatozni. Premda je neuobičajen, pseći karcinosarkom trebalo bi uzeti u obzir kao diferencijalnu dijagnozu kod miješanih tumora mliječne žlijezde, ako sadržavaju obje komponente. U dosadašnjim istraživanjima nema mnogo radova o metastazama karcinosarkoma mliječne žlijezde kuja, posebno u slučajevima gdje je to agresivan tumor. Stoga smatramo da je ovaj prikaz slučaja vrijedan doprinos kojim su utvrđene višestruke metastaze karcinosarkoma mliječne žlijezde na različitim organima

Discutindo a educação ambiental no cotidiano escolar: desenvolvimento de projetos na escola formação inicial e continuada de professores

A presente pesquisa buscou discutir como a Educação Ambiental (EA) vem sendo trabalhada, no Ensino Fundamental e como os docentes desta escola compreendem e vem inserindo a EA no cotidiano escolar., em uma escola estadual do município de Tangará da Serra/MT, Brasil. Para tanto, realizou-se entrevistas com os professores que fazem parte de um projeto interdisciplinar de EA na escola pesquisada. Verificou-se que o projeto da escola não vem conseguindo alcançar os objetivos propostos por: desconhecimento do mesmo, pelos professores; formação deficiente dos professores, não entendimento da EA como processo de ensino-aprendizagem, falta de recursos didáticos, planejamento inadequado das atividades. A partir dessa constatação, procurou-se debater a impossibilidade de tratar do tema fora do trabalho interdisciplinar, bem como, e principalmente, a importância de um estudo mais aprofundado de EA, vinculando teoria e prática, tanto na formação docente, como em projetos escolares, a fim de fugir do tradicional vínculo “EA e ecologia, lixo e horta”.Facultad de Humanidades y Ciencias de la Educació

Histopathological findings and immunohistochemical distribution of bacterial antigen in Balb/c mice experimentally infected with Arcobacter butzleri

The aim of this study was to determine the tissue colonization and pathogenicity of Arcobacter butzleri in BALB/c mice. For this purpose, 60 adult mice were distributed into three experimental groups consisting of 16 animals in each and into three control groups consisting of 4 animals in each. One ml of A. butzleri suspension containing 1x10(9) CFU/mL was inoculated by peroral (PO), intraperitoneal (IP) and intramammary (IMG) routes to BALB/c mice in experimental groups in order to determine the clinical and pathological findings. Animals in control groups received 1 mL of sterile saline solution. Duration of the experiment was limited for 15 days. The BALB/c mice were found to be susceptible to infection by the three ways of inoculation. In infected mice, diarrhea, weakness, decrease of body temperature (IP and IMG); hepatomegaly, splenomegaly (IP); swelling of mammary glands, exudation, abscess formation (IMG) were seen. Mortality was determined as 25% in PO inoculation group and as 95% in IP IMG inoculation groups. In histopathological examination, acute/chronic inflammation characterized with neutrophil and plasma cells and local necrosis or congestion (IP) were determined in the lungs, kidneys, regional lymph nodes, liver, spleen, stomach and colon. However, severe, diffuse and chronic pyogranulomatous and necrotizing inflammation and interstitial fibrosis were observed in mammary gland and its subcutaneous tissues (IMG). In all of inoculation groups, A. butzleri was reisolated until the 5th days of post inoculation. The results of this study have suggested that BALB/c mice might be an appropriate experimental animal model for studying A. butzleri infection

An abdominal cavity abscess associated with Salmonella enterica serovar Typhimurium phage type DT2 in a dog: a case report

Most Salmonella enterica serovar Typhimurium strains are capable of infecting multiple hosts. In this report, an abdominal cavity abscess associated with the Salmonella enterica serovar Typhimurium phage type DT2 is described in a dog. A dead male dog was admitted to the Department of Pathology at the Faculty of Veterinary Medicine at Erciyes University for necropsy. Anorexia, weight loss and lethargy were the clinical symptoms that were reported by the owner of the dog. The diagnosis was made by histopathological and bacteriological examinations of the lungs, spleen, liver and heart. In addition, the content of the abdominal cavity mass was evaluated in bacteriological analysis. The serotyping, phage typing and antibiotic susceptibility of the isolated bacteria were performed at the Danish Institute for Food and Veterinary Research. To the best of the authors' knowledge, this is the first study reporting an abscess associated with S. enterica serovar Typhimurium phage type DT2 in a dog