The spontaneous and induced synthesis of Epstein-Barr virus antigens in Raji cells immobilized on surface coated with anti-lymphocyte globulin

Immobilization of Raji cells on surface coated with anti-lymphocyte globulin (ALG) at low cell densities lead to the synthesis of Epstein-Barr virus (EBV) early antigen (EA) in up to 5% of the cells. At higher cell densities the percentage of antigen-positive cells decreased and at confluency no antigen synthesis was observed. Addition of iododeoxyuridine (IdUrd) to low density cultures increased the expression of EA to 20%, whereas in confluent cultures the cells could not be induced to synthesize EA. Treatment of cells in suspension with ALG failed to induced EA synthesis and did not potentiate the effect of IdUrd. Immobilized Raji cells proved to be suitable targets for superinfection with EBV derived from P3HR1 cultures

The regulated expression of Epstein-Barr virus. III. Proteins specified by EBV during the lytic cycle

The experiments show that 30 virus-induced or virus-specified proteins were synthesized in Raji cells after superinfection with Epstein-Barr virus (EBV) derived from P3HR1 cells. Using a combination of pulse labelling, application of cycloheximide blocks at different times post-infection, treatment with amino acid analogues and inhibition of DNA synthesis it was shown that three groups of proteins appear in Raji cells after superinfection; the synthesis of the proteins in any one group appears to be coordinately regulated. Amongst the six virus-induced proteins which were synthesized immediately after release from an early cycloheximide block one would expect to find those proteins essential for the transition from EBNA to EA synthesis. Using human sera with differing specificities for the various antigen groups 11 proteins were identified as being specifically precipitated by sera having high titres against the EBV-induced early antigen complex

A Spin-Orbit Alignment for the Hot Jupiter HATS-3b

We have measured the alignment between the orbit of HATS-3b (a recently discovered, slightly inflated Hot Jupiter) and the spin-axis of its host star. Data were obtained using the CYCLOPS2 optical-fiber bundle and its simultaneous calibration system feeding the UCLES spectrograph on the Anglo-Australian Telescope. The sky-projected spin-orbit angle of $\lambda = 3\pm25^{\circ}$ was determined from spectroscopic measurements of Rossiter-McLaughlin effect. This is the first exoplanet discovered through the HATSouth transit survey to have its spin-orbit angle measured. Our results indicate that the orbital plane of HATS-3b is consistent with being aligned to the spin axis of its host star. The low obliquity of the HATS-3 system, which has a relatively hot mid F-type host star, agrees with the general trend observed for Hot Jupiter host stars with effective temperatures $>6250$K to have randomly distributed spin-orbit angles.Comment: 13 pages. Accepted for publication in the Astrophysical Journa

Ks band secondary eclipses of WASP-19b and WASP-43b with the Anglo-Australian Telescope

We report new Ks band secondary eclipse observations for the hot-Jupiters WASP-19b and WASP-43b. Using the IRIS2 infrared camera on the Anglo-Australian Telescope (AAT), we measured significant secondary eclipses for both planets, with depths of 0.287 -0.020/+0.020% and 0.181 -0.027/+0.027% for WASP-19b and WASP-43b respectively. We compare the observations to atmosphere models from the VSTAR line-by-line radiative transfer code, and examine the effect of C/O abundance, top layer haze, and metallicities on the observed spectra. We performed a series of signal injection and recovery exercises on the observed light curves to explore the detection thresholds of the AAT+IRIS2 facility. We find that the optimal photometric precision is achieved for targets brighter than Kmag = 9, for which eclipses as shallow as 0.05% are detectable at >5 sigma significance.Comment: Accepted for publication in MNRAS, 13 pages, 10 figure

Planets in Spin-Orbit Misalignment and the Search for Stellar Companions

The discovery of giant planets orbiting close to their host stars was one of the most unexpected results of early exoplanetary science. Astronomers have since found that a significant fraction of these 'Hot Jupiters' move on orbits substantially misaligned with the rotation axis of their host star. We recently reported the measurement of the spin-orbit misalignment for WASP-79b by using data from the 3.9 m Anglo-Australian Telescope. Contemporary models of planetary formation produce planets on nearly coplanar orbits with respect to their host star's equator. We discuss the mechanisms which could drive planets into spin-orbit misalignment. The most commonly proposed being the Kozai mechanism, which requires the presence of a distant, massive companion to the star-planet system. We therefore describe a volume-limited direct-imaging survey of Hot Jupiter systems with measured spin-orbit angles, to search for the presence of stellar companions and test the Kozai hypothesis.Comment: Accepted for publication in the peer-reviewed proceedings of the 13th annual Australian Space Science Conferenc

A Nearly Polar Orbit for the Extrasolar Hot Jupiter WASP-79b

We report the measurement of a spin-orbit misalignment for WASP-79b, a recently discovered, bloated transiting hot Jupiter from the WASP survey. Data were obtained using the CYCLOPS2 optical-fiber bundle and its simultaneous calibration system feeding the UCLES spectrograph on the Anglo-Australian Telescope. We have used the Rossiter-McLaughlin effect to determine the sky-projected spin-orbit angle to be lambda = -106+19-13 degrees. This result indicates a significant misalignment between the spin axis of the host star and the orbital plane of the planet -- the planet being in a nearly polar orbit. WASP-79 is consistent with other stars that have Teff > 6250K and host hot Jupiters in spin-orbit misalignment.Comment: 8 pages, 2 figures, in press ApJL (accepted 2 August 2013

A Mesolithic settlement site at Howick, Northumberland: a preliminary report

Excavations at a coastal site at Howick during 2000 and 2002 have revealed evidence for a substantial Mesolithic settlement and a Bronze Age cist cemetery. Twenty one radiocarbon determinations of the earlier eighth millennium BP (Cal.) indicate that the Mesolithic site is one of the earliest known in northern Britain. An 8m core of sediment was recovered from stream deposits adjacent to the archaeological site which provides information on local environmental conditions. Howick offers a unique opportunity to understand aspects of hunter-gatherer colonisation and settlement during a period of rapid palaeogeographical change around the margins of the North Sea basin, at a time when it was being progressively inundated by the final stages of the postglacial marine transgression. The cist cemetery will add to the picture of Bronze Age occupation of the coastal strip and again reveals a correlation between the location of Bronze Age and Mesolithic sites which has been observed elsewhere in the region

[OII] emitters in the GOODS field at z~1.85: a homogeneous measure of evolving star formation

We present the results of a deep, near-infrared, narrow band imaging survey at a central wavelength of 1.062 microns (FWHM=0.01 microns) in the GOODS-South field using the ESO VLT instrument, HAWK-I. The data are used to carry out the highest redshift search for [OII]3727 emission line galaxies to date. The images reach an emission line flux limit (5 sigma) of 1.5 x 10^-17 erg cm^-2 s^-1, additionally making the survey the deepest of its kind at high redshift. In this paper we identify a sample of [OII]3727 emission line objects at redshift z~1.85 in a co-moving volume of ~4100 Mpc^3. Objects are selected using an observed equivalent width (EW_obs) threshold of EW_obs = 50 angstroms. The sample is used to derive the space density and constrain the luminosity function of [OII] emitters at z=1.85. We find that the space density of objects with observed [OII] luminosities in the range log(L_[OII]) > 41.74 erg s^-1 is log(rho)=-2.45+/-0.14 Mpc^-3, a factor of 2 greater than the observed space density of [OII] emitters reported at z~1.4. After accounting for completeness and assuming an internal extinction correction of A_Halpha=1 mag (equivalent to A_[OII]=1.87), we report a star formation rate density of rho* ~0.38+/-0.06 Msun yr^-1 Mpc^-3. We independently derive the dust extinction of the sample using 24 micron fluxes and find a mean extinction of A_[OII]=0.98+/-0.11 magnitudes (A_Halpha=0.52). This is significantly lower than the A_Halpha=1 (A[OII]=1.86) mag value widely used in the literature. Finally we incorporate this improved extinction correction into the star formation rate density measurement and report rho*~0.24+/-0.06 Msun yr^-1 Mpc^-3.Comment: 11 pages, 10 figures, accepted for publication in MNRA

Secure, performance-oriented data management for nanoCMOS electronics

The EPSRC pilot project Meeting the Design Challenges of nanoCMOS Electronics (nanoCMOS) is focused upon delivering a production level e-Infrastructure to meet the challenges facing the semiconductor industry in dealing with the next generation of ‘atomic-scale’ transistor devices. This scale means that previous assumptions on the uniformity of transistor devices in electronics circuit and systems design are no longer valid, and the industry as a whole must deal with variability throughout the design process. Infrastructures to tackle this problem must provide seamless access to very large HPC resources for computationally expensive simulation of statistic ensembles of microscopically varying physical devices, and manage the many hundreds of thousands of files and meta-data associated with these simulations. A key challenge in undertaking this is in protecting the intellectual property associated with the data, simulations and design process as a whole. In this paper we present the nanoCMOS infrastructure and outline an evaluation undertaken on the Storage Resource Broker (SRB) and the Andrew File System (AFS) considering in particular the extent that they meet the performance and security requirements of the nanoCMOS domain. We also describe how metadata management is supported and linked to simulations and results in a scalable and secure manner

EML4-ALK variants: biological and molecular properties, and the implications for patients

Since the discovery of the fusion between EML4 (echinoderm microtubule associated protein-like 4) and ALK (anaplastic lymphoma kinase), EML4-ALK, in lung adenocarcinomas in 2007, and the subsequent identification of at least 15 different variants in lung cancers, there has been a revolution in molecular-targeted therapy that has transformed the outlook for these patients. Our recent focus has been on understanding how and why the expression of particular variants can affect biological and molecular properties of cancer cells, as well as identifying the key signalling pathways triggered, as a result. In the clinical setting, this understanding led to the discovery that the type of variant influences the response of patients to ALK therapy. Here, we discuss what we know so far about the EML4-ALK variants in molecular signalling pathways and what questions remain to be answered. In the longer term, this analysis may uncover ways to specifically treat patients for a better outcome