Uso del tiempo, cuidados y bienestar : desafíos de Uruguay y la región

Editoria

Characterization of nitrophospholipid-peptide covalent adducts by electrospray tandem mass spectrometry: a first screening analysis using different instrumental platforms

Lipids are well‐known targets of reactive nitrogen species and this reaction leads to the formation of nitrated lipids that have been associated with anti‐inflammatory and cytoprotective effects. Nitro‐fatty acids (NO2‐FA) are highly electrophilic compounds that can form covalent adducts with proteins, leading to the formation of lipoxidation adducts, which modulate the protein structure and function. Nitrated phospholipids (NO2‐PL) have been detected recently in biological samples, but their biological effects are unknown, although similarly to what has been described for nitrated lipids, it has been hypothesized that they may react with peptides and proteins. In this study, in vitro biomimetic assays are used to synthetize adducts of nitrated POPC (NO2POPC), already detected in biological samples, and GSH peptide. The formation of NO2POPC‐GSH adducts is studied by ESI‐MS and MS2, using both low and high energy CID in different MS platforms: a LXQ linear ion trap, a Q‐TOF 2, and a Q‐Exactive Hybrid Quadrupole‐Orbitrap. Typical product ions observed under MS2 conditions are modified b, y, and C ions bearing NO2POPC covalently linked that unequivocally confirms the presence of the lipid‐peptide adduct. Typical loss of HNO2 is only observed in the MS2 of the mono‐charged precursor ions, [M+H]+. Product ions at m/z 184 or neutral loss of 183 Da are assigned as typical fragmentations that confirm the presence of the phosphatidylcholine. In summary, the characterization of nitro PL‐peptide adducts by MS and MS2 allows the identification of the structure and specific MS2 reporter ions to be used to pinpoint these adductions in biological systems.publishe

Paradoxical lucidity: A potential paradigm shift for the neurobiology and treatment of severe dementias

Unexpected cognitive lucidity and communication in patients with severe dementias, especially around the time of death, have been observed and reported anecdotally. Here, we review what is known about this phenomenon, related phenomena that provide insight into potential mechanisms, ethical implications, and methodologic considerations for systematic investigation. We conclude that paradoxical lucidity, if systematically confirmed, challenges current assumptions and highlights the possibility of networkâ level return of cognitive function in cases of severe dementias, which can provide insight into both underlying neurobiology and future therapeutic possibilities.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/153062/1/alzjjalz201904002.pd

Arabic validation of the Compulsive Internet Use Scale (CIUS)

<p>Abstract</p> <p>Background</p> <p>The popularity of using the Internet and related applications has grown in Arabic countries in recent years. Despite numerous advantages in terms of optimizing communications among individuals and social systems, the use of the Internet may in certain cases become problematic and engender negative consequences in daily life. As no instrument in the Arabic language is available, however, to measure excessive Internet use, the goal of the current study was to validate an Arabic version of the Compulsive Internet Use Scale (CIUS).</p> <p>Methods</p> <p>The Arabic version of the CIUS was administered to a sample of 185 Internet users and exploratory and confirmatory analyses performed.</p> <p>Results</p> <p>As found previously for the original version, a one-factor model of the CIUS had good psychometric properties and fit the data well. The total score on the CIUS was positively associated with time spent online.</p> <p>Conclusion</p> <p>The Arabic version of the CIUS seems to be a valid self-report to measure problematic Internet use.</p

Olives and olive oil are sources of electrophilic fatty acid nitroalkenes

Extra virgin olive oil (EVOO) and olives, key sources of unsaturated fatty acids in the Mediterranean diet, provide health benefits to humans. Nitric oxide (•NO) and nitrite (NO2-)-dependent reactions of unsaturated fatty acids yield electrophilic nitroalkene derivatives (NO 2-FA) that manifest salutary pleiotropic cell signaling responses in mammals. Herein, the endogenous presence of NO2-FA in both EVOO and fresh olives was demonstrated by mass spectrometry. The electrophilic nature of these species was affirmed by the detection of significant levels of protein cysteine adducts of nitro-oleic acid (NO2-OA-cysteine) in fresh olives, especially in the peel. Further nitration of EVOO by NO2- under acidic gastric digestive conditions revealed that human consumption of olive lipids will produce additional nitro-conjugated linoleic acid (NO2-cLA) and nitro-oleic acid (NO2-OA). The presence of free and protein-adducted NO2-FA in both mammalian and plant lipids further affirm a role for these species as signaling mediators. Since NO2-FA instigate adaptive anti-inflammatory gene expression and metabolic responses, these redox-derived metabolites may contribute to the cardiovascular benefits associated with the Mediterranean diet. © 2014 Fazzari et al

La dépendance à Internet – phénoménologie et approches thérapeutiques

Die Frage, was Internetsucht eigentlich ist und worauf sie sich bezieht, ist komplex und vielschichtig. Das Internet selbst, die Medienangebote und deren Vernetzung befinden sich in einem ständigen Wandlungsprozess. Um das Phänomen der Verhaltenssüchte einordnen und beschreiben zu können, zeichnet sich zudem ein Trend zur Abkehr vom klassischen Begriff der Suchterkrankung hin zu einer konzeptuell weiter gefassten Kategorie von Sucht ab. Eine Individualisierung der Therapie und Fokussierung auf die von den Betroffenen gesuchten und als Belohnung empfunden Inhalte, und die Entwicklung von effektiven Bewältigungsstrategien sind wesentliche therapeutische Ansätze.Internet addiction – phenomenology and therapeutic interventionsThe question of what Internet addiction actually is and what it pertains to is complex and has multiple aspects. The Internet itself, the media offers and their interconnections are in a constant process of transformation. In order to classify the phenomenon in terms of a behavioural addiction and to be able to describe it, there is a trend of moving away from the classical concept of an addictive illness towards an inclusive conceptually broader category of addiction. A significant part of the therapeutic interventions is invidualising the therapy focusing on which contents the affected individual seeks out and which contents are experienced as rewarding and the development of effective coping strategies

Trabajo y cuidado infantil (un desafío exclusivamente femenino ? Una mirada desde el género y la ciudadanía social)

ST QUENTIN EN YVELINES-BU (782972101) / SudocPARIS-INED-Documentation (751202301) / SudocSudocFranceF

Viktor E. Frankl - Gesammelte Werke: Psychotherapie, Psychiatrie und Religion

The fifth Volume of the Edition of the Collected Works of Viktor Frankls unites Frankl’s writings, notes, lectures and interviews on the border area of psychology, psychiatry, and religion.Der fünfte Band der Edition der Gesammelten Werke von Viktor Frankl vereint Frankls Schriften, Vorträge, Notizen und Gespräche über den Grenzbereich zwischen Psychologie, Psychiatrie und Religion