Ecology and conservation of Alseuosmia quercifolia (Alseuosmiaceae) in the Waikato region, New Zealand

The ecology of Alseuosmia quercifolia, a small endemic shrub, was investigated, focussing on its habitat requirements, population dynamics, phenology and reproductive biology, and conservation status. This species occurs most commonly in lowland native forests of the Waikato region of the North Island (north of latitude 38°05'S), but is also found in scattered populations to North Cape. In the Waikato region it typically occupies shady, well-drained, south or south-east facing lower slopes of hills and ranges at altitudes below 400 m. Population structures show considerable variation amongst seven study sites in the Waikato region, with disjunct size classes a reflection of the presence and abundance of introduced browsing mammals. It is a relatively short-lived (less than 50 years), slow-growing species with a fleshy fruit adapted to bird dispersal, but seed dispersal now appears to be primarily by gravity. Flowering occurs early in spring and is synchronous at both individual and population levels, occurring over a 5-week period, with peak flowering during the second and third weeks. While all populations set seed, reproductive output can be negatively affected by persistent browse and by rain during peak flowering. This species is vulnerable because it is highly palatable to introduced mammals and all plants in a population are within browse height. It has relatively narrow habitat specificity, localised distribution, and limited potential to extend its range. We suggest it fulfils the requirements of the category "declining", using the most recent classification of threatened and uncommon plants of New Zealand

Derivation of supply curves for catchment water effluents meeting specific salinity concentration targets in 2050: linking farm and catchment level models or “Footprints on future salt / water planes”

The salt burden in a stream reflects the blend of salty and fresh flows from different soil areas in its catchment. Depending not only on long-run rainfall, water yields from a soil are also determined by land cover: lowest if the area is forested and greatest if cleared. Water yields under agro-forestry, lucerne pasture, perennial grass pasture, and annual pasture or cropping options span the range of water yields between the extremes of forested and cleared lands. This study explores quantitative approaches for connecting the hydrologic and economic consequences of farm-level decisions on land cover (productive land uses) to the costs of attaining different catchment level targets of water volumes and salt reaching downstream users; environmental, agricultural, domestic, commercial and industrial. This connection is critical for the resolution of the externality dilemma of meeting downstream demands for water volume and quality. New technology, new products and new markets will expand options for salinity abatement measures in the dryland farming areas of watershed catchments. The development of appropriate policy solutions to address demands for water volumes and quality depends on the possibility of inducing targeted land use change in those catchments or parts of catchments where decreased saline flows or increased fresh water flows can return the best value for money. This study provides such a link.salinity, targets, opportunity cost, concentration, dilution, effluent, externality, supply, demand, policy, water quality, new technology, new markets, Resource /Energy Economics and Policy,

Mathematical optimisation of drainage and economic land use for target water and salt yields

Land managers in upper catchments are being asked to make expensive changes in land use, such as by planting trees, to attain environmental service targets, including reduced salt loads in rivers, to meet needs of downstream towns, farms and natural habitats. End-of-valley targets for salt loads have sometimes been set without a quantitative model of cause and effect regarding impacts on water yields, economic efficiency or distribution of costs and benefits among stakeholders. This paper presents a method for calculating a ‘menu’ of technically feasible options for changes from current to future mean water yields and salt loads from upstream catchments having local groundwater flow systems, and the land-use changes to attain each of these options at minimum cost. It sets the economic stage for upstream landholders to negotiate with downstream parties future water-yield and salt-load targets, on the basis of what it will cost to supply these ecosystem services.discounting, landuse, NPV, opportunity-cost, salinity, Resource /Energy Economics and Policy,

A Coding Variant in the Gene Bardet-Biedl Syndrome 4 (BBS4) Is Associated with a Novel Form of Canine Progressive Retinal Atrophy

Progressive retinal atrophy is a common cause of blindness in the dog and affects >100 breeds. It is characterized by gradual vision loss that occurs due to the degeneration of photoreceptor cells in the retina. Similar to the human counterpart retinitis pigmentosa, the canine disorder is clinically and genetically heterogeneous and the underlying cause remains unknown for many cases. We use a positional candidate gene approach to identify putative variants in the Hungarian Puli breed using genotyping data of 14 family-based samples (CanineHD BeadChip array, Illumina) and whole-genome sequencing data of two proband and two parental samples (Illumina HiSeq 2000). A single nonsense SNP in exon 2 of BBS4 (c.58A > T, p.Lys20*) was identified following filtering of high quality variants. This allele is highly associated (P-CHISQ = 3.425e(-14), n = 103) and segregates perfectly with progressive retinal atrophy in the Hungarian Puli. In humans, BBS4 is known to cause Bardet-Biedl syndrome which includes a retinitis pigmentosa phenotype. From the observed coding change we expect that no functional BBS4 can be produced in the affected dogs. We identified canine phenotypes comparable with Bbs4-null mice including obesity and spermatozoa flagella defects. Knockout mice fail to form spermatozoa flagella. In the affected Hungarian Puli spermatozoa flagella are present, however a large proportion of sperm are morphologically abnormal andPeer reviewe

Negative impact of β-arrestin-1 on post-myocardial infarction heart failure via cardiac and adrenal-dependent neurohormonal mechanisms

β-Arrestin (βarr)-1 and β-arrestin-2 (βarrs) are universal G-protein-coupled receptor adapter proteins that negatively regulate cardiac β-adrenergic receptor (βAR) function via βAR desensitization and downregulation. In addition, they mediate G-protein-independent βAR signaling, which might be beneficial, for example, antiapoptotic, for the heart. However, the specific role(s) of each βarr isoform in cardiac βAR dysfunction, the molecular hallmark of chronic heart failure (HF), remains unknown. Furthermore, adrenal βarr1 exacerbates HF by chronically enhancing adrenal production and hence circulating levels of aldosterone and catecholamines. Herein, we sought to delineate specific roles of βarr1 in post-myocardial infarction (MI) HF by testing the effects of βarr1 genetic deletion on normal and post-MI cardiac function and morphology. We studied βarr1 knockout (βarr1KO) mice alongside wild-type controls under normal conditions and after surgical MI. Normal (sham-operated) βarr1KO mice display enhanced βAR-dependent contractility and post-MI βarr1KO mice enhanced overall cardiac function (and βAR-dependent contractility) compared with wild type. Post-MI βarr1KO mice also show increased survival and decreased cardiac infarct size, apoptosis, and adverse remodeling, as well as circulating catecholamines and aldosterone, compared with post-MI wild type. The underlying mechanisms, on one hand, improved cardiac βAR signaling and function, as evidenced by increased βAR density and procontractile signaling, via reduced cardiac βAR desensitization because of cardiac βarr1 absence, and, on the other hand, decreased production leading to lower circulating levels of catecholamines and aldosterone because of adrenal βarr1 absence. Thus, βarr1, via both cardiac and adrenal effects, is detrimental for cardiac structure and function and significantly exacerbates post-MI HF

The Development of Chemical and Biological Profiling for the Forensic Provenancing of Norfolk Soils

Soils are frequently analysed by forensic laboratories by comparing a suspect sample to an especially collected control sample. As yet, they cannot be compared to a central database, unless the area in question has already been identified; with the use databases being highly contested within the field of forensic geosciences. There is a need for a method of soil profiling that allows an unknown sample to be tested and assigned a quantitative likelihood that it originated from a given region. Spatial models can then be created using geographical information systems to house multiple datasets and be used to map soils across geographical areas. Generally, the more variables available with which to compare items, the greater the certainty a forensic analyst can have when asserting their similarity; this applies to geological materials. Equally, soil profiling methods can be used to exclude soil samples from each other or an area. This research involves a number of chemical and biological profiling methods that have been used to build up a unique signature for soils from different locations across Norfolk. All analyses have been carried out on a single source sample. 87Sr/86Sr ratios have been measured using MC-ICP-MS, and trace element concentrations measured using ICP-MS. The 87Sr/86Sr ratios are significantly different at each of the sample locations; although there is some variation in the replicates collected at each location this variation is smaller than the regional variation. The correlation between the isotope chemistry of the topsoil and the underlying geology is poor, indicating that other sources such as land-use, vegetation cover and additions to the soil contribute to the 87Sr/86Sr. Therefore, trace element concentrations have been used to spatially discriminate samples and to investigate the effect of fertilisers on the elemental composition of the topsoil. The biological techniques used to aid discrimination are soil DNA analysis using the chloroplast-located matK gene and MALDI-ToF-MS, palynology and the creation of Norfolk vegetation maps showing all of the plant species recorded in the area; each additional independent dataset allows for an increasing signature of each sample to be built up which can be used for assessing similarity or exclusionary purposes

Activation of relaxin family receptor 1 from different mammalian species by relaxin peptide and small-molecule agonist ML290

Relaxin peptide (RLN), which signals through the relaxin family peptide 1 (RXFP1) GPCR receptor, has shown therapeutic effects in an acute heart failure clinical trial. We have identified a small-molecule agonist of human RXFP1, ML290; however, it does not activate the mouse receptor. To find a suitable animal model for ML290 testing and to gain mechanistic insights into the interaction of various ligands with RXFP1, we have cloned rhesus macaque, pig, rabbit, and guinea pig RXFP1s and analyzed their activation by RLN and ML290. HEK293T cells expressing macaque or pig RXFP1 responded to relaxin and ML290 treatment as measured by an increase of cAMP production. Guinea pig RXFP1 responded to relaxin but had very low response to ML290 treatment only at highest concentrations used. The rabbit RXFP1 amino acid sequence was the most divergent, with a number of unique substitutions within the ectodomain and the seven-transmembrane domain (7TM). Two splice variants of rabbit RXFP1 derived through alternative splicing of the fourth exon were identified. In contrast to the other species, rabbit RXFP1s were activated by ML290, but not with human, pig, mouse, or rabbit RLNs. Using FLAG-tagged constructs, we have shown that both rabbit RXFP1 variants are expressed on the cell surface. No binding of human Eu-labeled RLN to rabbit RXFP1 was detected, suggesting that in this species, RXFP1 might be non-functional. We used chimeric rabbit–human and guinea pig–human constructs to identify regions important for RLN or ML290 receptor activation. Chimeras with the human ectodomain and rabbit 7TM domain were activated by RLN, whereas substitution of part of the guinea pig 7TM domain with the human sequence only partially restored ML290 activation, confirming the allosteric mode of action for the two ligands. Our data demonstrate that macaque and pig models can be used for ML290 testing

The Role of Autobiographies in Activism: How Former 'Comfort Women' Used Testimonies to Fight for Their Rights

Imagine you are in a room of women from different cultures and backgrounds. You may be inclined to think that these women have nothing in common. However, if you were to ask them if they had experienced sexual harassment, four of out five (81%) would raise their hands (Chatterjee). According to the National Sexual Violence Resource Center, one in five women have also been raped. Sexual violence defines the female experience. Female sexuality has always been centered around and dictated by men, their desires, and their sexual urges. Rebecca Solnit says, “sex is an arena of power” (108) and has long been a way to shame, control, and own women. Men have defined when, with whom, and under what circumstances women are allowed to have sex, and through an emphasis on female virginity, have controlled and regulated women and their bodies (Jung, Soh, and Solnit). Sex is one of the most basic components of human existence, and to exert control over someone’s sexuality is to take away their most basic freedom and their sense of security

High levels of childhood obesity observed among 3- to 7-year-old New Zealand Pacific children is a public health concern.

This cross-sectional, community-based survey was designed to assess attained growth and body composition of 3- to 7-y-old Pacific children (n = 21 boys and 20 girls) living in Dunedin, New Zealand, and to examine nondietary factors associated with the percentage of body fat. Fat mass, lean tissue mass and the percentage of body fat were measured using dual energy X-ray absorptiometry. One trained anthropometrist also measured height, weight, skinfolds (triceps, subscapular) and circumferences (mid-upper arm, chest, waist, calf). Compared with the National Center for Health Statistics and National Health and Examination Surveys I and II reference data, these Pacific children were tall and heavy for their age with high arm-muscle-area-for-height. Median (quartiles) Z-scores for height and BMI-for-age and arm-muscle-area-for-height were 1.33 (0.60, 2.15), 1.20 (0.74, 4.43) and 1.09 (0.63, 1.85), respectively. Their median (quartile) percentage of body fat was 21.8% (15.0, 35.5) of which 38.5% was located in the trunk. The estimated percentage of children classified as obese ranged from 34 to 49% depending on the criterion used. Over 60% of the children had levels of trunk fat above 1 SD of reported age- and sex-specific Z-scores for New Zealand children. The nondietary factors examined (hours of television viewing and hours playing organized sports, as reported by parents) were not associated with variations in the percentage of body fat, after adjusting for age, sex and birth weight. These extremely high levels of obesity and truncal fat among very young New Zealand children will have major public health implications as these children age