Comparative genomics of the Liberibacter genus reveals widespread diversity in genomic content and positive selection history

‘Candidatus Liberibacter’ is a group of bacterial species that are obligate intracellular plant pathogens and cause Huanglongbing disease of citrus trees and Zebra Chip in potatoes. Here, we examined the extent of intra- and interspecific genetic diversity across the genus using comparative genomics. Our approach examined a wide set of Liberibacter genome sequences including five pathogenic species and one species not known to cause disease. By performing comparative genomics analyses, we sought to understand the evolutionary history of this genus and to identify genes or genome regions that may affect pathogenicity. With a set of 52 genomes, we performed comparative genomics, measured genome rearrangement, and completed statistical tests of positive selection. We explored markers of genetic diversity across the genus, such as average nucleotide identity across the whole genome. These analyses revealed the highest intraspecific diversity amongst the ‘Ca. Liberibacter solanacearum’ species, which also has the largest plant host range. We identified sets of core and accessory genes across the genus and within each species and measured the ratio of nonsynonymous to synonymous mutations (dN/dS) across genes. We identified ten genes with evidence of a history of positive selection in the Liberibacter genus, including genes in the Tad complex, which have been previously implicated as being highly divergent in the ‘Ca. L. capsica’ species based on high values of dN

Improving irrigation efficiency will be insufficient to meet future water demand in the Nile Basin

The Nile River Basin covers an area of approximately 3.2 million km2 and is shared by 11 countries. Rapid population growth is expected in the region. The irrigation requirements of Nile riparian countries of existing (6.4 million ha) and additional planned (3.8 million ha, 2050) irrigation schemes were calculated, and the likely water savings through improved irrigation efficiency were evaluated. We applied SPARE:WATER to calculate irrigation demands on the basis of the well-known FAO56 Crop Irrigation Guidelines. Egypt (67 km3 yr-1) and Sudan (19 km3 yr-1) consume the highest share of the 84 km3 yr-1 total (2011). Assuming today’s poor irrigation infrastructure, the total consumption was predicted to increase to 123 km3 yr-1 (2050), an amount far exceeding the total annual yield of the Nile Basin. Therefore, a key challenge for water resources management in the Nile Basin is balancing the increasing irrigation water demand basin-wide with the available water supply. We found that water savings from improved irrigation technology will not be able to meet the additional needs of planned areas. Under a theoretical scenario of maximum possible efficiency, the deficit would still be 5 km3 yr-1. For more likely efficiency improvement scenarios, the deficit ranged between 23 and 29 km3 yr-1. Our results suggest that that improving irrigation efficiency may substantially contribute to decreasing water stress on the Nile system but would not completely meet the demand. Study Region: The Nile River Basin covers an area of approximately 3.2 million km2 and is shared by 11 countries. Rapid population growth is expected in the region. Study Focus: Record population growth is expected for the study region. Therefore, the irrigation requirements of Nile riparian countries of existing (6.4 million ha) and additional planned (3.8 million ha, 2050) irrigation schemes were calculated, and likely water savings through improved irrigation efficiency were evaluated. We applied a spatial decision support system (SPARE:WATER) to calculate the irrigation demands on the basis of the well-known FAO56 Crop Irrigation Guidelines. New Hydrological Insights for the Region: Egypt (67 km3yr-1) and Sudan (19 km3yr-1) consume the highest share of 84 km3yr-1 (2011). Assuming today’s poor irrigation infrastructure, the total demand were predicted to increase to 123 km3yr-1 (2050), an amount far exceeding the total annual yield of the Nile Basin. Therefore, a key challenge for water resources management in the Nile Basin is balancing the increasing irrigation water demand and available water supply. We found that water savings from improved irrigation technology will not be able to meet the additional needs of planned areas. Under a theoretical scenario of maximum possible efficiency, the deficit would still be 5 km3yr-1. For more likely efficiency improvement scenarios, the deficit ranges between 23 and 29 km3yr-1. Our results suggest that improving irrigation efficiency may substantially contribute to decreasing water stress on the Nile system but would not completely meet the demand

Analiza dizajna LCLC rezonantnog invertera za dvostupanjsko dvofazno napajanje

This paper deals with the design analysis and synthesis of power resonant inverter with sinusoidal output voltage for sensitive loads. The proposed filter must be capable of removing higher harmonic components from the supplying voltage to reach a harmonic distortion of roughly 5% in the whole range of the load (0 − 100%). The inverter can be supplied from either single-phase voltage inverter in full- or half- bridge connection, or from simple DC/DC buck converter. Non-symmetrical control causes higher harmonic content, both odd and even. Simulation and experimental results based on designed parameters and subsequently obtained from Matlab and OrCad models confirm good quality of output quantities, voltage and current.Tema je ovog članka analiza dizajna i sinteza učinskog rezonantnog invertera sa sinisuidalnim izlaznim naponom za osjetljive terete. Predloženi filtar mora moći filtrirati više harmonike ulaznog napona kako bi distorzija harmonika bila oko 5% u čitavom radnom području (0−100%). Inverter se može napajati ili iz jednofaznog naponskog invertera u mosnom ili uzrokuje pojavu viših harmonika u signalu, kako parnih tako i neparnih. Simulacijski i eksperimentalni rezultati temeljeni na sintetiziranim parametrima dobivenim od modela napravljenih u programskim paketima Matlab i OrCad potvr.uju dobru kvalitetu izlaznih veličina napona i struje

The Endocrine Disruptor Mono-(2-Ethylhexyl) Phthalate Affects the Differentiation of Human Liposarcoma Cells (SW 872)

Esters of phthalic acid (phthalates) are largely used in industrial plastics, medical devices, and pharmaceutical formulations. They are easily released from plastics into the environment and can be found in measurable levels in human fluids. Phthalates are agonists for peroxisome proliferator-activated receptors (PPARs), through which they regulate translocator protein (TSPO; 18 kDa) transcription in a tissue-specific manner. TSPO is a drug- and cholesterol-binding protein involved in mitochondrial respiration, steroid formation, and cell proliferation. TSPO has been shown to increase during differentiation and decrease during maturation in mouse adipocytes. The purpose of this study was to establish the effect of mono-(2-ethylhexyl) phthalate (MEHP) on the differentiation of human SW 872 preadipocyte cells, and examine the role of TSPO in the process. After 4 days of treatment with 10 µM MEHP, we observed changes in the transcription of acetyl-CoA carboxylase alpha, adenosine triphosphate citrate lyase, glucose transporters 1 and 4, and the S100 calcium binding protein B, all of which are markers of preadipocyte differentiation. These observed gene expression changes coincided with a decrease in cellular proliferation without affecting cellular triglyceride content. Taken together, these data suggest that MEHP exerts a differentiating effect on human preadipocytes. Interestingly, MEHP was able to temporarily increase TSPO mRNA levels through the PPAR-α and β/δ pathways. These results suggest that TSPO can be considered an important player in the differentiation process itself, or alternatively a factor whose presence is essential for adipocyte development

Serotonin transporter (SERT) and translocator protein (TSPO) expression in the obese ob/ob mouse

Background: An ever growing body of evidences is emerging concerning metabolism hormones, neurotransmitters or stress-related biomarkers as effective modulators of eating behavior and body weight in mammals. The present study sought at examining the density and affinity of two proteins related to neurotransmission and cell metabolism, the serotonin transporter SERT and the cholesterol import-benzodiazepine site TSPO (translocator protein), in a rodent leptin-lacking mutant, the obese ob/ob mouse. Binding studies were thus carried out in brain or peripheral tissues, blood platelets (SERT) and kidneys (TSPO), of ob/ob and WT mice supplied with a standard diet, using the selective radiochemical ligands [(3)H]-paroxetine and [(3)H]-PK11195. Results: We observed comparable SERT number or affinity in brain and platelets of ob/ob and WT mice, whilst a significantly higher [(3)H]-PK11195 density was reported in the brain of ob/ob animals. TSPO binding parameters were similar in the kidneys of all tested mice. By [(3)H]-PK11195 autoradiography of coronal hypothalamic-hippocampal sections, an increased TSPO signal was detected in the dentate gyrus (hippocampus) and choroids plexus of ob/ob mice, without appreciable changes in the cortex or hypothalamic-thalamic regions. Conclusions: These findings show that TSPO expression is up-regulated in cerebral regions of ob/ob leptin-deficient mice, suggesting a role of the translocator protein in leptin-dependent CNS trophism and metabolism. Unchanged SERT in mutant mice is discussed herein in the context of previous literature as the forerunner to a deeper biochemical investigation

Tumor Suppression by RNA from C/EBPβ 3′UTR through the Inhibition of Protein Kinase Cε Activity

BACKGROUND: Since the end of last century, RNAs from the 3'untranslated region (3'UTR) of several eukaryotic mRNAs have been found to exert tumor suppression activity when introduced into malignant cells independent of their whole mRNAs. In this study, we sought to determine the molecular mechanism of the tumor suppression activity of a short RNA from 3'UTR of C/EBPβ mRΝΑ (C/EBPβ 3'UTR RNA) in human hepatocarcinoma cells SMMC-7721. METHODOLOGY/PRINCIPAL FINDINGS: By using Western blotting, immunocytochemistry, molecular beacon, confocal microscopy, protein kinase inhibitors and in vitro kinase assays, we found that, in the C/EBPβ 3'UTR-transfectant cells of SMMC-7721, the overexpressed C/EBPβ 3'UTR RNA induced reorganization of keratin 18 by binding to this keratin; that the C/EBPβ 3'UTR RNA also reduced phosphorylation and expression of keratin 18; and that the enzyme responsible for phosphorylating keratin 18 is protein kinase Cε. We then found that the C/EBPβ 3'UTR RNA directly inhibited the phosphorylating activity of protein kinase Cε; and that C/EBPβ 3'UTR RNA specifically bound with the protein kinase Cε-keratin 18 conjugate. CONCLUSION/SIGNIFICANCE: Together, these facts suggest that the tumor suppression in SMMC-7721 by C/EBPβ 3'UTR RNA is due to the inhibition of protein kinase Cε activity through direct physical interaction between C/EBPβ 3'UTR RNA and protein kinase Cε. These facts indicate that the 3'UTR of some eukaryotic mRNAs may function as regulators for genes other than their own

Current paradigm of the 18-kDa translocator protein (TSPO) as a molecular target for PET imaging in neuroinflammation and neurodegenerative diseases

Neuroinflammation is a process characterised by drastic changes in microglial morphology and by marked upregulation of the 18-kDa translocator protein (TSPO) on the mitochondria. The continual increase in incidence of neuroinflammation and neurodegenerative diseases poses a major health issue in many countries, requiring more innovative diagnostic and monitoring tools. TSPO expression may constitute a biomarker for brain inflammation that could be monitored by using TSPO tracers as neuroimaging agents. From medical imaging perspectives, this review focuses on the current concepts related to the TSPO, and discusses briefly on the status of its PET imaging related to neuroinflammation and neurodegenerative diseases in humans

Alterations in voltage-sensing of the mitochondrial permeability transition pore in ANT1-deficient cells

The probability of mitochondrial permeability transition (mPT) pore opening is inversely related to the magnitude of the proton electrochemical gradient. The module conferring sensitivity of the pore to this gradient has not been identified. We investigated mPT's voltage-sensing properties elicited by calcimycin or H2O2 in human fibroblasts exhibiting partial or complete lack of ANT1 and in C2C12 myotubes with knocked-down ANT1 expression. mPT onset was assessed by measuring in situ mitochondrial volume using the 'thinness ratio' and the 'cobalt-calcein' technique. De-energization hastened calcimycin-induced swelling in control and partially-expressing ANT1 fibroblasts, but not in cells lacking ANT1, despite greater losses of mitochondrial membrane potential. Matrix Ca(2+) levels measured by X-rhod-1 or mitochondrially-targeted ratiometric biosensor 4mtD3cpv, or ADP-ATP exchange rates did not differ among cell types. ANT1-null fibroblasts were also resistant to H2O2-induced mitochondrial swelling. Permeabilized C2C12 myotubes with knocked-down ANT1 exhibited higher calcium uptake capacity and voltage-thresholds of mPT opening inferred from cytochrome c release, but intact cells showed no differences in calcimycin-induced onset of mPT, irrespective of energization and ANT1 expression, albeit the number of cells undergoing mPT increased less significantly upon chemically-induced hypoxia than control cells. We conclude that ANT1 confers sensitivity of the pore to the electrochemical gradient