Assessing climate change risks and contextual vulnerability in urban areas of semi-arid India the case of Bangalore

This paper disaggregates multiple climate change-urban linkages into key components through a generic ‘urban risk framework’. It further contextualises this framework within a fast-growing city (Bangalore) environment, in a semi-arid ecosystem to demonstrate the range of risks and vulnerabilities that are both unique and generic to many other Indian cities. It elucidates the ‘sensitivity’ and argues that the risk management framework could be used as a lever to bring elements of adaptation and mitigation responses together so that existing and emerging climatic and non-climatic risks can be addressed

How do we assess vulnerability to climate change in India : a systematic review of literature

This work was carried out under the Collaborative Adaptation Research Initiative in Africa and Asia (CARIAA), with financial support from the UK Government’s Department for International Development (DfID) and the International Development Research Centre (IDRC), CanadaIn India, several vulnerability assessment tools have been designed spanning multiple disciplines, by multiple actors, and at multiple scales. However, their conceptual, methodological, and disciplinary underpinnings, and resulting implications on who is identified as vulnerable, have not been interrogated. Addressing this gap, we systematically review peer-reviewed publications (n = 78) and grey literature (n = 42) to characterise how vulnerability to climate change is assessed in India. We frame our enquiry against four questions: (1) How is vulnerability conceptualised (vulnerability of whom/what, vulnerability to what), (2) who assesses vulnerability, (3) how is vulnerability assessed (methodology, scale), and (4) what are the implications of methodology on outcomes of the assessment. Our findings emphasise that methods to assess vulnerability to climate change are embedded in the disciplinary traditions, methodological approaches, and often-unstated motivations of those designing the assessment

Vertical integration for climate change adaptation in the water sector:lessons from decentralisation in Africa and India

Vertical integration, which creates strategic linkages between national and sub-national levels, is being promoted as important for climate change adaptation. Decentralisation, which transfers authority and responsibility to lower levels of organisation, serves a similar purpose and has been in place for a number of decades. Based on four case studies in semi-arid regions in Africa and India, this paper argues that vertical integration for climate change adaptation should reflect on lessons from decentralisation related to governing natural resources, particularly in the water sector. The paper focuses on participation and flexibility, two central components of climate change adaptation, and considers how decentralisation has enhanced or undermined these. The findings suggest that vertical integration for adaptation will be strengthened if a number of lessons are considered, namely (i) actively seek equitable representation from marginal and diverse local groups drawing on both formal and informal participation structures, (ii) assess and address capacity deficits that undermine flexibility and adaptive responses, especially within lower levels of government, and (iii) use hybrid modes of governance that include government, intermediaries and diverse local actors through both formal and informal institutions to improve bottom-up engagement

Livelihood vulnerability and adaptation in Kolar District, Karnataka, India : mapping risks and responses short report

This work was carried out under the Collaborative Adaptation Research Initiative in Africa and Asia (CARIAA), with financial support from the UK Government’s Department for International Development (DfID) and the International Development Research Centre (IDRC), Canada.During March and April 2016, ASSAR India’s researchers from the Indian Institute for Human Settlements (IIHS) conducted 18 Focus Group Discussions (FGDs) in nine villages in Kolar District, Karnataka. The FGDs were gender-differentiated and ensured representation from different income groups, castes, and religions. We undertook three activities during each FGD: - A timeline exercise to chart biophysical, livelihoods, socio-economic, institutional and political changes from 1970 onwards. - Risk and response mapping. - An institutional mapping exercise to chart key actors and flows of information and credit

Compact IF2 allows initiator tRNA accommodation into the P site and gates the ribosome to elongation

Abstract During translation initiation, initiation factor 2 (IF2) holds initiator transfer RNA (fMet-tRNAi fMet) in a specific orientation in the peptidyl (P) site of the ribosome. Upon subunit joining IF2 hydrolyzes GTP and, concomitant with inorganic phosphate (Pi) release, changes conformation facilitating fMet-tRNAi fMet accommodation into the P site and transition of the 70 S ribosome initiation complex (70S-IC) to an elongation-competent ribosome. The mechanism by which IF2 separates from initiator tRNA at the end of translation initiation remains elusive. Here, we report cryo-electron microscopy (cryo-EM) structures of the 70S-IC from Pseudomonas aeruginosa bound to compact IF2-GDP and initiator tRNA. Relative to GTP-bound IF2, rotation of the switch 2 α-helix in the G-domain bound to GDP unlocks a cascade of large-domain movements in IF2 that propagate to the distal tRNA-binding domain C2. The C2-domain relocates 35 angstroms away from tRNA, explaining how IF2 makes way for fMet-tRNAi fMet accommodation into the P site. Our findings provide the basis by which IF2 gates the ribosome to the elongation phase

Structure of S. pombe telomerase protein Pof8 C-terminal domain is an xRRM conserved among LARP7 proteins

La-related proteins 7 (LARP7) are a class of RNA chaperones that bind the 3' ends of RNA and are constitutively associated with their specific target RNAs. In metazoa, Larp7 binds to the long non-coding 7SK RNA as a core component of the 7SK RNP, a major regulator of eukaryotic transcription. In the ciliate Tetrahymena the LARP7 protein p65 is a component of telomerase, an essential ribonucleoprotein complex that maintains the telomeric DNA at eukaryotic chromosome ends. p65 is important for the ordered assembly of telomerase RNA (TER) with telomerase reverse transcriptase. Unexpectedly, Schizosaccharomyces pombe Pof8 was recently identified as a LARP7 protein and a core component of fission yeast telomerase essential for biogenesis. LARP7 proteins have a conserved N-terminal La motif and RRM1 (La module) and C-terminal RRM2 with specific RNA substrate recognition attributed to RRM2, first structurally characterized in p65 as an atypical RRM named xRRM. Here we present the X-ray crystal structure and NMR studies of S. pombe Pof8 RRM2. Sequence and structure comparison of Pof8 RRM2 to p65 and human Larp7 xRRMs reveals conserved features for RNA binding with the main variability in the length of the non-canonical helix α3. This study shows that Pof8 has conserved xRRM features, providing insight into TER recognition and the defining characteristics of the xRRM

Structure of Telomerase with Telomeric DNA.

Telomerase is an RNA-protein complex (RNP) that extends telomeric DNA at the 3' ends of chromosomes using its telomerase reverse transcriptase (TERT) and integral template-containing telomerase RNA (TER). Its activity is a critical determinant of human health, affecting aging, cancer, and stem cell renewal. Lack of atomic models of telomerase, particularly one with DNA bound, has limited our mechanistic understanding of telomeric DNA repeat synthesis. We report the 4.8 Å resolution cryoelectron microscopy structure of active Tetrahymena telomerase bound to telomeric DNA. The catalytic core is an intricately interlocked structure of TERT and TER, including a previously structurally uncharacterized TERT domain that interacts with the TEN domain to physically enclose TER and regulate activity. This complete structure of a telomerase catalytic core and its interactions with telomeric DNA from the template to telomere-interacting p50-TEB complex provides unanticipated insights into telomerase assembly and catalytic cycle and a new paradigm for a reverse transcriptase RNP

Structural basis of transcription initiation by bacterial RNA polymerase holoenzyme

12 p.-5 fig.-1 tab.The bacterial RNA polymerase (RNAP) holoenzyme containing σ factor initiates transcription at specific promoter sites by de novo RNA priming, the first step of RNA synthesis where RNAP accepts two initiating ribonucleoside triphosphates (iNTPs) and performs the first phosphodiester bond formation. We present the structure of de novo transcription initiation complex that reveals unique contacts of the iNTPs bound at the transcription start site with the template DNA and also with RNAP and demonstrate the importance of these contacts for transcription initiation. To get further insight into the mechanism of RNA priming, we determined the structure of initially transcribing complex of RNAP holoenzyme with 6-mer RNA, obtained by in crystallo transcription approach. The structure highlights RNAP-RNA contacts that stabilize the short RNA transcript in the active site and demonstrates that the RNA 5'-end displaces σ region 3.2 from its position near the active site, which likely plays a key role in σ ejection during the initiation-to-elongation transition. Given the structural conservation of the RNAP active site, the mechanism of de novo RNA priming appears to be conserved in all cellular RNAPs.This work was supported, in whole or in part, by National Institutes of Health Grant GM087350-A1 (to K. S. M.). This work was also supported in part by the Spanish government through Grant BFU2010-16336 (to C. F.-T.). This work was also supported by the Russian Foundation for Basic Research (Grants 14-04-01696 and 14-04-31994) and the Russian Academy of Sciences Presidium Program in Molecular and Cellular Biology (to A. K.).Peer reviewe