Boring bacteria: a morphological research on bone diagenesis.

Post mortem interval (PMI) estimation is a crucial issue in forensic medicine. To date, little is known about factors affecting post-mortem changes in hard tissues and it is still unclear who between exogenous bacteria from the environment and endogenous microbiota is the cause of microscopical alterations observed in human bone after death. Recent research highlighted an important role of endogenous bacteria in the earlier stages of the process. The aim of this study was to probe a potential endogenous model of human bone biodeterioration, based on the action of oral cavity endogenous microorganisms. A total of seventy-four fragments of human bone samples were incubated with six bacterial strains, isolated from human tartar specimens. In a forty-eight months long prospective study, the onset and development of bone tissue alterations were serially analysed by scanning electron microscope. The research furnished evidence that endogenous bacteria are able to bore into human dead bone, giving rise to microstructural changes morphologically indistinguishable from those observed in archaeological and forensic bone

Boring bacteria: a morphological research on bone diagenesis.

Post mortem interval (PMI) estimation is a crucial issue in forensic medicine. To date, little is known about factors affecting post-mortem changes in hard tissues and it is still unclear who between exogenous bacteria from the environment and endogenous microbiota is the cause of microscopical alterations observed in human bone after death. Recent research highlighted an important role of endogenous bacteria in the earlier stages of the process. The aim of this study was to probe a potential endogenous model of human bone biodeterioration, based on the action of oral cavity endogenous microorganisms. A total of seventy-four fragments of human bone samples were incubated with six bacterial strains, isolated from human tartar specimens. In a forty-eight months long prospective study, the onset and development of bone tissue alterations were serially analysed by scanning electron microscope. The research furnished evidence that endogenous bacteria are able to bore into human dead bone, giving rise to microstructural changes morphologically indistinguishable from those observed in archaeological and forensic bone

Boring bacteria: a morphological research on bone diagenesis.

Post mortem interval (PMI) estimation is a crucial issue in forensic medicine. To date, little is known about factors affecting post-mortem changes in hard tissues and it is still unclear who between exogenous bacteria from the environment and endogenous microbiota is the cause of microscopical alterations observed in human bone after death. Recent research highlighted an important role of endogenous bacteria in the earlier stages of the process. The aim of this study was to probe a potential endogenous model of human bone biodeterioration, based on the action of oral cavity endogenous microorganisms. A total of seventy-four fragments of human bone samples were incubated with six bacterial strains, isolated from human tartar specimens. In a forty-eight months long prospective study, the onset and development of bone tissue alterations were serially analysed by scanning electron microscope. The research furnished evidence that endogenous bacteria are able to bore into human dead bone, giving rise to microstructural changes morphologically indistinguishable from those observed in archaeological and forensic bone

Multiple Organ Dysfunction Syndrome (MODS) induced by Candida krusei in an Aldabra giant tortoise (Aldabrachelys gigantea) and confirmed by electron microscopy analysis

A young female Aldabra giant tortoise (Adabrachelys gigantea) was presented with anorexia, ataxia, severe constipation and bloating. Analysis revealed liver disease and collected biopsy diagnosed Candida krusei infection. Despite Itraconazole treatment, the tortoise got worse and died. Full necropsy was performed; microbiology showed Candida krusei presence in liver, but histopathology didn’t confirm fungal presence with special stains, so scanning electron microscopy was essential to prove a detailed diagnosis of extensive mycosis. Keywords: Giant tortoise, Constipation, Electron microscopy, Candida, Liver diseas

A novel disorder involving dyshematopoiesis, inflammation, and HLH due to aberrant CDC42 function

Hemophagocytic lymphohistiocytosis (HLH) is characterized by immune dysregulation due to inadequate restraint of overactivated immune cells and is associated with a variable clinical spectrum having overlap with more common pathophysiologies. HLH is difficult to diagnose and can be part of inflammatory syndromes. Here, we identify a novel hematological/autoinflammatory condition (NOCARH syndrome) in four unrelated patients with superimposable features, including neonatal-onset cytopenia with dyshematopoiesis, autoinflammation, rash, and HLH. Patients shared the same de novo CDC42 mutation (Chr1:22417990CT, p.R186C) and altered hematopoietic compartment, immune dysregulation, and inflammation. CDC42 mutations had been associated with syndromic neurodevelopmental disorders. In vitro and in vivo assays documented unique effects of p.R186C on CDC42 localization and function, correlating with the distinctiveness of the trait. Emapalumab was critical to the survival of one patient, who underwent successful bone marrow transplantation. Early recognition of the disorder and establishment of treatment followed by bone marrow transplant are important to survival