Issues Determining the Development of Cruise Itineraries: A Focus on the Luxury Market

While the cruise industry has been identified as an ever increasingly important aspect of the wider tourism industry, this sector remains relatively under researched. Cruises have shown strong year on year growth and, in 2004, was the holiday of choice for more than 12 million tourists worldwide. Challenges facing the industry are concerned with market segmentation and the development of new and exciting ports of call. This research focuses on one aspect of the luxury cruise industry: that of the development of cruise itineraries. Specifically, this research will identify the itinerary planning process in the luxury cruise industry, determine the main issues and challenges faced when developing itineraries and finally present an overview of the influences that might shape luxury cruise itinerary planning in the future

Disappearance of Residual Dry Matter on Annual Grassland in the Absence of Grazing

Residual dry matter (RDM) is a standard used by grassland managers for assessing the level of grazing use on annual grasslands and associated savannas and woodlands. Residual dry matter is the old plant material left standing or on the ground at the beginning of a new growing season. It indicates the combined effects of the previous season\u27s forage production and its consumption by grazing animals of all types. The standard assumes that the amount of RDM remaining in the fall, subject to site conditions and variations in weather, will influence subsequent species composition and forage production, in addition to providing soil protection and protect against nutrient losses (Bartolome, et al., 2002). While RDM is measured at the beginning of a new growing season, grazing does not always occur continuously up to this time. Managers do not have information to predict the disappearance of residual dry matter due to physical and chemical breakdown during a period of non-grazing. In this study the rate of RDM disappearance during the summer (non-growing) period on annual grasslands was investigated

A Three-Dimensional Dynamic Supramolecular "Sticky Fingers" Organic Framework.

Engineering high-recognition host-guest materials is a burgeoning area in basic and applied research. The challenge of exploring novel porous materials with advanced functionalities prompted us to develop dynamic crystalline structures promoted by soft interactions. The first example of a pure molecular dynamic crystalline framework is demonstrated, which is held together by means of weak "sticky fingers" van der Waals interactions. The presented organic-fullerene-based material exhibits a non-porous dynamic crystalline structure capable of undergoing single-crystal-to-single-crystal reactions. Exposure to hydrazine vapors induces structural and chemical changes that manifest as toposelective hydrogenation of alternating rings on the surface of the [60]fullerene. Control experiments confirm that the same reaction does not occur when performed in solution. Easy-to-detect changes in the macroscopic properties of the sample suggest utility as molecular sensors or energy-storage materials

RNA-directed DNA methylation involves co-transcriptional small-RNA-guided slicing of polymerase V transcripts in Arabidopsis.

Small RNAs regulate chromatin modifications such as DNA methylation and gene silencing across eukaryotic genomes. In plants, RNA-directed DNA methylation (RdDM) requires 24-nucleotide small interfering RNAs (siRNAs) that bind to ARGONAUTE 4 (AGO4) and target genomic regions for silencing. RdDM also requires non-coding RNAs transcribed by RNA polymerase V (Pol V) that probably serve as scaffolds for binding of AGO4-siRNA complexes. Here, we used a modified global nuclear run-on protocol followed by deep sequencing to capture Pol V nascent transcripts genome-wide. We uncovered unique characteristics of Pol V RNAs, including a uracil (U) common at position 10. This uracil was complementary to the 5' adenine found in many AGO4-bound 24-nucleotide siRNAs and was eliminated in a siRNA-deficient mutant as well as in the ago4/6/9 triple mutant, suggesting that the +10 U signature is due to siRNA-mediated co-transcriptional slicing of Pol V transcripts. Expression of wild-type AGO4 in ago4/6/9 mutants was able to restore slicing of Pol V transcripts, but a catalytically inactive AGO4 mutant did not correct the slicing defect. We also found that Pol V transcript slicing required SUPPRESSOR OF TY INSERTION 5-LIKE (SPT5L), an elongation factor whose function is not well understood. These results highlight the importance of Pol V transcript slicing in RNA-mediated transcriptional gene silencing, which is a conserved process in many eukaryotes

A post hoc pooled analysis of exacerbations among US participants in randomized controlled trials of tiotropium

SummaryBackgroundExacerbations are a defining outcome of chronic obstructive pulmonary disease (COPD). We evaluated the effect of tiotropium on COPD exacerbations and related hospitalizations among patients from the USA enrolled in clinical trials.MethodsData were pooled from six randomized, double-blind, placebo-controlled trials (6 to ≥12 months' duration) of tiotropium in patients with COPD. Exacerbations were defined retrospectively as an increase in or new onset of >1 respiratory symptom lasting for ≥3 days and requiring treatment with systemic corticosteroids and/or antibiotics. Time to first exacerbation or hospitalization and exacerbation rates were analyzed at 6 months, and at 1 year for studies ≥1 year.ResultsIn total, 4355 patients (tiotropium, 2268, placebo, 2087; mean age 66.5 years; forced expiratory volume in 1 s [FEV1] 1.03 L [35.5% predicted]) were analyzed at 6 months and 2455 at 1 year (tiotropium 1317, placebo 1138; mean age 65.5 years; FEV1 1.03 L [37.0% predicted]). Tiotropium delayed time to first exacerbation or first hospitalized exacerbation at 6 months (hazard ratios [HRs], 0.80, 0.65, respectively; p < 0.001 vs placebo) and 1 year (HRs, 0.73 and 0.55; p < 0.001 vs placebo) and reduced exacerbation rates and hospitalization rates (6 months: HRs, 0.79, 0.64; 1 year: HRs, 0.78, 0.56, respectively; all p < 0.01 vs placebo). Tiotropium significantly reduced exacerbations, irrespective of inhaled corticosteroid use at baseline. Tiotropium was not associated with an increased risk of cardiac-related events.ConclusionsTiotropium significantly reduced the risk and rates of exacerbations and hospitalizations among US patients with COPD

Comparison of direct and indirect methods to maximise the detection of Babesia caballi and Theileria equi infections in Central Southern Italy

: Equine piroplasmosis is a disease of equids, caused by tick-borne apicomplexan protozoan pathogens Babesia caballi and Theileria equi, which, according to the World Organisation for Animal Health (OIE), can be diagnosed by enzyme-linked immunosorbent assay (ELISA), immunofluorescent antibody test (IFAT) and polymerase chain reaction (PCR). The present study was conducted to evaluate and compare the assays available for the diagnosis of equine piroplasmosis. Data employed were obtained from 1300 blood samples collected between 2012-2014 from asymptomatic and symptomatic equines (horses and donkeys) of central-southern regions of Italy and analyzed by ELISA, IFAT, PCR (one commercial and one from literature) and blood smear microscopic examination. Statistical differences of the proportions of positivity for each parasite and group (asymptomatic and symptomatic) among the methods were verified by the z test to identify the most sensitive. The concordance between each pair of methods - for each parasite and within the groups - and trends in detection of suspect samples of four hypothetical diagnostic algorithms using serological and biomolecular assays were evaluated to identify the most suitable laboratory diagnostic workflow. The results of this study highlighted a lower capacity to detect suspect samples of commercial ELISA for B. caballi in all groups when compared to biomolecular methods and IFAT; and of the commercial PCRs in asymptomatic animals, identifying a PCR from literature and IFAT as the best choice for a combined diagnosis. For T. equi, IFAT detected more suspect samples than ELISA, even if the latter showed good performance and some samples were positive only by the ELISA and PCR, indicating that their simultaneous employment is still advantageous. Host-parasite interaction, amino-acid/genetic diversity and differences in detection limits among the assays could be among the reasons in explaining the present results. In view of further studies, ELISA should be used in combination with PCR, that should regularly be included in the laboratory diagnosis to maximise the detection of early infections and support the evaluation of pharmacological treatment

Animal growth, tissular composition, and carcass conformation of light Merino lambs

Neither the age (average 74.3 ± 2.9 days) nor the arrival weight of males and females (average 15.38 ± 0.25 kg) at the fattening farm, after the lactation period, were statistically significant. At the end of the fattening period, statistically significant differences were found for the weights and growth of the two sexes (25.91 ± 0.59 kg vs. 22.52 ± 0.52 kg (p-value: 0.000), and 242.15 ± 10.15 g/day vs. 178.51 ± 9.86 g/day (p-value: 0.006) in males and females respectively). Regarding carcass conformation measurements, the differences between sexes were statistically significant for: K, G and Th, but not F. In the carcass performance, there were significant differences between males (7.5 ± 0.3) vs. females (6.7 ± 0.2) (p-value: 0.023), which means that the males had a rating of R- and the females O+. Regarding tissue composition, only the percentage of muscle was significantly higher in males (58.50 ± 0.88) than in females (52.94 ± 0.76), (p-value: 0.011). After the logical differences between males and females, the study showed that both sexes had a higher growth potential than the final weights at which the study was carried out, since they did not show a high fattening (22.76 ± 0.86 percentage). Our results were in accordance with other authors.This research has been financed by a collaboration agreement between the Agricultural Technological Institute of Castilla and León (ITA- CYL), the University of Valladolid (UVa) and the Scientific Park Foundation of the UVa and the project AGL2016-75159-C2-1-R funded by the Spanish State Research Agency (AEI) and the European Regional Development FundLight lambGrowthCarcassMerino breedUnpublishe

Emerging Diluted Ferromagnetism in High-T-c Superconductors Driven by Point Defect Clusters

Defects in ceramic materials are generally seen as detrimental to their functionality and applicability. Yet, in some complex oxides, defects present an opportunity to enhance some of their properties or even lead to the discovery of exciting physics, particularly in the presence of strong correlations. A paradigmatic case is the high-temperature superconductor YBa2Cu3O7-delta(Y123), in which nanoscale defects play an important role as they can immobilize quantized magnetic flux vortices. Here previously unforeseen point defects buried in Y123 thin films that lead to the formation of ferromagnetic clusters embedded within the superconductor are unveiled. Aberration-corrected scanning transmission microscopy has been used for exploring, on a single unit-cell level, the structure and chemistry resulting from these complex point defects, along with density functional theory calculations, for providing new insights about their nature including an unexpected defect-driven ferromagnetism, and X-ray magnetic circular dichroism for bearing evidence of Cu magnetic moments that align ferromagnetically even below the superconducting critical temperature to form a dilute system of magnetic clusters associated with the point defects