1,816 research outputs found
Association of Bcl-2 with misfolded prion protein is linked to the toxic potential of cytosolic PrP
Protein misfolding is linked to different neurodegenerative disorders like Alzheimer’s disease, polyglutamine, and prion diseases. We investigated the cytotoxic effects of aberrant conformers of the prion protein (PrP) and show that toxicity is specifically linked to misfolding of PrP in the cytosolic compartment and involves binding of PrP to the anti-apoptotic protein Bcl-2. PrP targeted to different cellular compartments, including the cytosol, nucleus, and mitochondria, adopted a misfolded and partially proteinase K–resistant conformation. However, only in the cytosol did the accumulation of misfolded PrP induce apoptosis. Apoptotic cell death was also induced by two pathogenic mutants of PrP, which are partially localized in the cytosol. A mechanistic analysis revealed that the toxic potential is linked to an internal domain of PrP (amino acids 115–156) and involves coaggregation of cytosolic PrP with Bcl-2. Increased expression of the chaperones Hsp70 and Hsp40 prevented the formation of PrP/Bcl-2 coaggregates and interfered with PrP-induced apoptosis. Our study reveals a compartment-specific toxicity of PrP misfolding that involves coaggregation of Bcl-2 and indicates a protective role of molecular chaperones
CASTOR: Centauro and Strange Object Research in nucleus-nucleus collisions at LHC
We describe the CASTOR detector designed to probe the very forward,
baryon-rich rapidity region in nucleus-nucleus collisions at the LHC. We
present a phenomenological model describing the formation of a QGP fireball in
a high baryochemical potential environment, and its subsequent decay into
baryons and strangelets. The model explains Centauros and the long-penetrating
component and makes predictions for the LHC.
Simulations of Centauro-type events were done. To study the response of the
apparatus to new effects different exotic species (DCC, Centauros, strangelets
etc.) were passed through the deep calorimeter. The energy deposition pattern
in the calorimeter appears to be a new clear signature of the QGP.Comment: Talk given by E. Gladysz-Dziadus for the CASTOR group, Intern.
Workshop on Nuclear Theory, 10-15 June, 2002, Bulgaria, Rila Mountains, 15
pages, 14 figure
Particle abundances and spectra in the hydrodynamical description of relativistic nuclear collisions with light projectiles
We show that a hydrodynamical model with continuous particle emission instead
of sudden freeze out may explain both the observed strange particle and pion
abundances and transverse mass spectra for light projectile at SPS energy. We
found that the observed enhancement of pion production corresponds, within the
context of continuous emission, to the maximal entropy production.Comment: 11 pages, 2 figure
Thermal Hadron Production in High Energy Heavy Ion Collisions
We provide a method to test if hadrons produced in high energy heavy ion
collisions were emitted at freeze-out from an equilibrium hadron gas. Our
considerations are based on an ideal gas at fixed temperature , baryon
number density , and vanishing total strangeness. The constituents of this
gas are all hadron resonances up to a mass of 2 GeV; they are taken to decay
according to the experimentally observed branching ratios. The ratios of the
various resulting hadron production rates are tabulated as functions of
and . These tables can be used for the equilibration analysis of any heavy
ion data; we illustrate this for some specific cases.Comment: 12 pages (not included :13 figures + tables) report CERN-TH 6523/92
and Bielefeld preprint BI-TP 92/0
Characterization of ovarian follicle reserve depletion in Ames dwarf mice.
Publicado: Proceedings of the 29th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Gramado, RS, Brazil, August 20th to 23rd, 2015, and 31st Meeting of the European Embryo Transfer Association (AETE); Ghent, Belgium, September 11th and 12th, 2015. Abstracts
Disruption of Growth Hormone Receptor Prevents Calorie Restriction from Improving Insulin Action and Longevity
Most mutations that delay aging and prolong lifespan in the mouse are related to somatotropic and/or insulin signaling. Calorie restriction (CR) is the only intervention that reliably increases mouse longevity. There is considerable phenotypic overlap between long-lived mutant mice and normal mice on chronic CR. Therefore, we investigated the interactive effects of CR and targeted disruption or knock out of the growth hormone receptor (GHRKO) in mice on longevity and the insulin signaling cascade. Every other day feeding corresponds to a mild (i.e. 15%) CR which increased median lifespan in normal mice but not in GHRKO mice corroborating our previous findings on the effects of moderate (30%) CR on the longevity of these animals. To determine why insulin sensitivity improves in normal but not GHRKO mice in response to 30% CR, we conducted insulin stimulation experiments after one year of CR. In normal mice, CR increased the insulin stimulated activation of the insulin signaling cascade (IR/IRS/PI3K/AKT) in liver and muscle. Livers of GHRKO mice responded to insulin by increased activation of the early steps of insulin signaling, which was dissipated by altered PI3K subunit abundance which putatively inhibited AKT activation. In the muscle of GHRKO mice, there was elevated downstream activation of the insulin signaling cascade (IRS/PI3K/AKT) in the absence of elevated IR activation. Further, we found a major reduction of inhibitory Ser phosphorylation of IRS-1 seen exclusively in GHRKO muscle which may underpin their elevated insulin sensitivity. Chronic CR failed to further modify the alterations in insulin signaling in GHRKO mice as compared to normal mice, likely explaining or contributing to the absence of CR effects on insulin sensitivity and longevity in these long-lived mice
Remnants of Initial Anisotropic High Energy Density Domains in Nucleus-Nucleus Collisions
Anisotropic high energy density domains may be formed at early stages of
ultrarelativistic heavy ion collisions, e.g. due to phase transition dynamics
or non-equilibrium phenomena like (mini-)jets. Here we investigate hadronic
observables resulting from an initially created anisotropic high energy density
domain. Based on our studies using a transport model we find that the initial
anisotropies are reflected in the freeze-out multiplicity distribution of both
pions and kaons due to secondary hadronic rescattering. The anisotropy appears
to be stronger for particles at high transverse momenta. The overall kaon
multiplicity increases with large fluctuations of local energy densities, while
no change has been found in the pion multiplicity.Comment: Submitted to PR
Enhancement of singly and multiply strangeness in p-Pb and Pb-Pb collisions at 158A GeV/c
The idea that the reduction of the strange quark suppression in string
fragmentation leads to the enhancement of strange particle yield in
nucleus-nucleus collisions is applied to study the singly and multiply strange
particle production in p-Pb and Pb-Pb collisions at 158A GeV/c. In this
mechanism the strange quark suppression factor is related to the effective
string tension, which increases in turn with the increase of the energy, of the
centrality and of the mass of colliding system. The WA97 observation that the
strange particle enhancement increases with the increasing of centrality and of
strange quark content in multiply strange particles in Pb-Pb collisions with
respect to p-Pb collisions was accounted reasonably.Comment: 8 pages, 3 PostScript figures, in Latex form. submitted to PR
Thermal phenomenology of hadrons from 200 AGeV S+S collisions
We develop a complete and consistent description for the hadron spectra from
heavy ion collisions in terms of a few collective variables, in particular
temperature, longitudinal and transverse flow. To achieve a meaningful
comparison with presently available data, we also include the resonance decays
into our picture. To disentangle the influences of transverse flow and
resonance decays in the -spectra, we analyse in detail the shape of the
-spectra.Comment: 31 pages, 13 figs in seperate uuencoded file, for LaTeX, epsf.sty and
dvips, TPR-93-16 and BNL-(no number yet
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