Cities: continuity, transformation, and emergence

Cities can be regarded as the quintessential example of complexity. Insofar as we can define a hidden hand determining their morphology, this is based on the glue that stitches together the actions of individuals and organizations who build the city from the ground-up, so-to-speak. When general systems theory entered the lexicon of science in the mid- 20th century, cities were regarded as being excellent examples of systems with interactions between basic elements that demonstrated the slogan of the field: the ‘whole is greater than the sum of the parts’. Since then, as complexity theory has evolved to embrace systems theory and as temporal dynamics has come onto the agenda, cities once again have been used to illustrate basic themes: global organization from local action, emergent morphology from simple spatial decision, temporal order at global levels from volatile, seemingly random change at the level of individual decision-making, evolution and progress through coevolution, competition, and endless variety. Here we will sketch these ideas with respect to cities illustrating particularly three key ideas which involve the tension between continuously changing systems, qualitative transformations, and radical change based on emergent properties of the whole. Our analysis has many implications for a new theory of urban planning which is built from the bottom up, rather than from the top down which is the traditional way in which such interventions are currently carried out in the name of making better cities. Contemporary problems such as ethnic segregation, urban sprawl, traffic congestion, urban decline, and regeneration are all informed by the perspective on complexity theory that we bring to bear here

Parto pré-termo tardio e de termo precoce em Portugal

Copyright © Ordem dos Médicos 2016Introduction: Late preterm birth (defined as birth between 34 and 36 complete weeks' gestation) and early term birth (defined as birth between 37 and 38 complete weeks' gestation) have become a topic of recent discussion as the morbidity associated with delivery at these gestational ages has become increasingly evident. Our objective was to evaluate the characteristics of late preterm and early term birth in Portugal. Material and methods: We developed a survey questionnaire that was sent to the Obstetric Department of all public hospitals in Portugal. The questionnaire consisted on questions on prevalence and mode of delivery of late preterm and early term period and associated neonatal morbidity and mortality. The questions referred solely to single births occurred during 2013. Results: We received completed questionnaires from 14 hospitals, corresponding to nearly one third (33.5%) of total deliveries in Portugal. We report 5.4% of late preterm and 27% of early term deliveries. Approximately two thirds of late preterm and three quarters of early term deliveries were spontaneous. The cesarean section rate was higher in late preterm (39.1%) than in early term (26.4%) births. Neonatal complications were more frequent in late preterm neonates (34.2%) when compared to early term neonates (14.2%). Discussion: The prevalence of late preterm and early term birth in our cohort is comparable, although slightly reduced, to other published series. Conclusion: The obstetric community should raise efforts to limit deliveries below 39 weeks' gestation to the ones with a valid medical indication.Introdução: Nos últimos anos, vários autores evidenciaram a morbilidade associada aos partos ocorridos entre as 34 e 36 semanas (pré-termo tardio) e entre as 37 e 38 semanas de gestação (termo precoce). Neste sentido, pretendemos realizar um estudo epidemiológico dos partos que ocorrem nestas idades gestacionais, em Portugal. Material e Métodos: Realizámos um inquérito, que foi aplicado a todos os hospitais públicos de Portugal, acerca da prevalência e via de parto nos partos pré-termo tardios e de termo precoce, e morbilidade e mortalidade neonatal associada. As questões referiam-se apenas a gestações de feto único e a partos ocorridos em 2013. Resultados: Incluímos 14 hospitais, correspondendo a 33,5% dos partos ocorridos em Portugal, em 2013. Verificámos que 5,4% dos partos ocorreram no período pré-termo tardio e 27% no termo precoce. Aproximadamente dois terços dos partos pré-termo tardio e três quartos dos partos de termo precoce foram espontâneos. A taxa de cesariana foi mais elevada entre as 34 e 36 semanas de gestação (39,1%) do que entre as 37 e 38 semanas (26,4%). As complicações neonatais foram mais frequentes após um parto pré-termo tardio (34,2%), quando comparadas com os de termo precoce (14,2%). Discussão: Na nossa amostra, a prevalência de parto pré-termo tardio e de termo precoce, ainda que ligeiramente inferior, é comparável à publicada em estudos anteriores. Conclusão: É importante que a comunidade obstétrica nacional adote atitudes no sentido de limitar os partos antes das 39 semanas de gestação. Assim, nestas idades gestacionais os partos devem possuir uma indicação médica válida.info:eu-repo/semantics/publishedVersio

A compact city for the wealthy? Employment accessibility inequalities between occupational classes in the London metropolitan region 2011

The prevalence of gentrification and housing marketisation processes in many cities points to increasingly wealthy inner-city areas and potentially greater population segregation by income. It is plausible that these trends are contributing to regional accessibility inequalities, though quantitative research testing this link is limited. This paper examines differences in employment accessibility between Standard Occupational Classification groups in the London Metropolitan Region for 2011 for car, transit, bus only and walking modes. Additionally, changes in occupational class populations 2006–2016 are considered, revealing continuing inner-city gentrification. Employment accessibility is calculated using cumulative measures, based on travel times from multi-modal network modelling. The results show that while car accessibility is relatively equal between occupational classes, public transport, bus and walk accessibility have significant inequalities favouring professional classes. Low income groups have lower accessibility for the most affordable bus and walk modes, and inequalities are greater for residents in the wider metropolitan region. Furthermore, professional groups combine accessibility advantages with the highest rates of owner occupation, maximising housing wealth benefits. Lower income groups are exposed to rent increases, though this is offset by social housing, which remains the most prevalent tenure in Inner London for low income classes

Preventing microbial infections with natural phenolic compounds

The struggle between humans and pathogens has taken and is continuing to take countless lives every year. As the misusage of conventional antibiotics increases, the complexity associated with the resistance mechanisms of pathogens has been evolving into gradually more clever mechanisms, diminishing the effectiveness of antibiotics. Hence, there is a growing interest in discovering novel and reliable therapeutics able to struggle with the infection, circumvent the resistance and defend the natural microbiome. In this regard, nature-derived phenolic compounds are gaining considerable attention due to their potential safety and therapeutic effect. Phenolic compounds comprise numerous and widely distributed groups with different biological activities attributed mainly to their structure. Investigations have revealed that phenolic compounds from natural sources exhibit potent antimicrobial activity against various clinically relevant pathogens associated with microbial infection and sensitize multi-drug resistance strains to bactericidal or bacteriostatic antibiotics. This review outlines the current knowledge about the antimicrobial activity of phenolic compounds from various natural sources, with a particular focus on the structure-activity relationship and mechanisms of actions of each class of natural phenolic compounds, including simple phenols, phenolic acids, coumarin, flavonoids, tannins, stilbenes, lignans, quinones, and curcuminoids.The authors acknowledge the financial support by the Portuguese Foundation for Science and Technology (FCT) through the doctoral grant and junior research contract with the reference number PD/BD/150521/2019 (K.E.) and CEECIND/01026/2018 (J.M.S.), respectively

Variations of Quality of Teacher–infant Interactions Across Play and Care Routine Activities

This study examined the extent to which the quality of teacher-infant interactions varies across play and routine care activities. In addition, the effects of the quantity of adult involvement in the quality of teacher-infant interactions were investigated. Participants were 90 infant classrooms in Portugal. Classrooms had, on average, 6 infants enrolled (M = 6.38, SD = 2.34), with the number of adults ranging from 1 to 3 (M = 2.00, SD = 0.60). Classrooms were observed by trained observers using the Classroom Assessment Scoring System–Infant (CLASS-Infant; Hamre et al., 2014). Research Findings: Multilevel models showed that interaction quality varied as a function of type of activity. The quality of interactions of all CLASS domains was lower in routine care activities compared to play activities. Findings further showed that adult involvement was positively associated with all CLASS domains. In addition, after adding adult involvement to the models, differences between play and routine care activities were no longer statistically significant for most CLASS domains. Practice or Policy: Findings suggest the importance of considering the context of the activity and the levels of adult involvement when assessing and improving the quality of teacher-infant interactions.info:eu-repo/semantics/publishedVersio

Chondrosia reniformis marine-sponge collagen membranes for skin re-epithelialization

Chondrosia reniformis collagen has been identified as mainly of type IV. Being collagen IV the main component of the epidermal basal layer [1], C. reniformis represents a valuable source to be explored in the skin regeneration field. This work envisaged the production of C. reniformis collagen membranes for the selection of rapidly adherent epidermal cells, like the commercial collagen coatings, and for their subsequent culture. This approach would permit a single system for culturing and carrying basal epidermal cells aimed at re-epithelialize skin wounds. Materials and Methods The collagen of C. reniformis marine-sponge was extracted with 100mM Tris-HCl, 10mM EDTA, 8M urea and 100mM 2-mercaptoethanol. To define the best re-solubilization conditions, the obtained precipitate was dissolved in five different solutions: Solution A: 100mM Tris-HCl+8M Urea+10mM EDTA (pH 9.5); Solution B: 50mM Tris-HCl+1M NaCl (pH 7.4); Solution C: 100mM Tris-HCl (pH 7.4); Solution D: 0.5% H2O2 (v/v) (pH 11) and Solution E: 100mM Tris-HCl (pH 9.5). Solutions of 1% collagen were prepared and cross-linking was performed with HMDI, genipin and EDC/NHS at different concentrations. The membranes were obtained by solvent-casting and/or freeze-drying, and their stability was tested both in PBS and culture medium, for at least 7 days. Morphological characterization of the membranes was carried out by scanning electron microscopy (SEM). Cytotoxicity, based on metabolic activity (MTS assay) and cell proliferation (DNA quantification) analysis of the 100mM Tris-HCl (pH 9.5) and 8mM EDC/NHS cross-linked collagen membranes, was assessed with L929 cells. Results were analyzed by IBM SPSS Statistics Version 20 using one-way ANOVA and Kruskall-Wallis test. Significance was set for

Achillea millefolium L. hydroethanolic extract inhibits growth of human tumor cell lines by interfering with cell cycle and inducing apoptosis

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Insights in non-Candida albicans Candida biofilms

The number of infections caused by Candida spp has greatly increased in the past years, which has been attributed to an increase in the number of AIDS and immunocompromised patients, in the elderly population and the more frequent use of indwelling medical devices. Most Candidiasis have been attributed to Candida albicans, however, recently, non‐Candida albicans Candida (NCAC) spp, as C. parapsilosis, C. glabrata and C. tropicalis, have been identified as common pathogens. Furthermore, Candida biofilm formation has important clinical repercussions due to their inherent tolerance to antifungal therapy and ability to withstand host immune defenses. Consequently, it is of utmost importance to understand the physiology and virulence of NCAC spp biofilms. Thus, the main aim of this work is to present some insights in C. parapsilosis, C. glabrata and C. tropicalis biofilms, through (i) biofilm characterization (structure and matrix composition); (ii) evaluation of antifungal agents tolerance and (iii) determination of putative virulence factors (extracellular enzymes and extracellular alcohols). SEM observation of Candida spp biofilms revealed that biofilm architecture was neither species nor strain dependent. However, C. glabrata biofilms, which presented lower biomass, formed, generally, a more compact and thick structure than C. tropicalis and C. parapsilosis ones. Regarding matrix composition, C. glabrata presented, in general, higher amounts of proteins and polysaccharides, in opposition to C. tropicalis that presented lower amounts of both components. Biofilms antifungal resistance tests revealed that C. glabrata biofilms present high resistance to fluconazole and itraconazole, in comparison with the other NCAC spp biofilms. With respect to putative virulence factors, the production of extracellular enzymes, namely proteases and phospholipases was also evaluated in C. tropicalis but there were no differences in the levels of enzymes production by biofilm and planktonic cells. Regarding the extracellular alcohols, it was found that C. parapsilosis and C. tropicalis produce farnesol, 1‐dodecanol, 2‐ phenylethanol and isoamyl alcohol (already described for C. albicans). Furthermore, the latter was produced by C. tropicalis in a higher amount than by C. parapsilosis. Overall, with this extensive research work it was possible to describe and relate several virulence features of NCAC spp with their putative virulence and invasiveness of human epithelia

The quality of caregiver–child interactions in infant classrooms in Portugal: the role of caregiver education

Recent research has shown that caregiver education and training can be important in determining levels of quality in early childhood, but has mainly considered the education and training of the lead teacher. In infant child care, however, classrooms have more than one caregiver with varying levels of education and in Portugal it is less common to have a qualified teacher exclusively assigned to infant classrooms. This study examines the quality of caregiver–child interactions in infant classrooms and its association with caregiver qualifications and training, specifically the level of pre-service education and in-service training of the lead caregiver, whether she is exclusively assigned to an infant classroom or to more classrooms, and the pre-service education of the multiple caregivers in the classroom. Participants were 90 infant classrooms from Porto, Portugal. The CLASS–Infant was used to measure quality of caregiver–infant interactions. Classrooms with one lead caregiver holding at least a bachelor’s degree, whether exclusively assigned to the infant classroom or not, showed higher levels of quality. Few effects were found for in-service training. Results suggest that lead teachers with pre-service education in early childhood are likely to play an important role not only directly by interacting with children but also indirectly through team work.info:eu-repo/semantics/publishedVersio