Generation, establishment and characterization of a pluripotent stem cell line (CVTTHi001-A) from primary fibroblasts isolated from a patient with activated PI3 kinase delta syndrome (APDS2)

APDS2 is caused by mutations in PIK3R1 gene resulting in constitutive PI3Kδ activation. PI3Kδ is predominantly expressed in leukocytes and plays critical roles in regulating immune responses. Here we first derived fibroblast primary cells from a skin biopsy of a patient carrying a heterozygous single T deletion in intron 11 of the PIK3R1 gene. We next present the derivation of an induced pluripotent stem cell (iPS) line using a non-integrative reprogramming technology. Pluripotent-related hallmarks are further shown, including: iPSCs self-renewal and expression of pluripotent and differentiation markers after in vitro differentiation towards embryonic germ layers, assessed by RT-PCR and immunofluorescence

Density-Dependent Effects of Simultaneous Root and Floral Herbivory on Plant Fitness and Defense

Supplementary Materials: The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/plants12020283/s1. Supplementary material 1, Table S1: Model structure and fit of GLMMs and GAMMs. Supplementary material 2, Figure S1: Plots with absolute values for analyzed variables. Supplementary material 3, Table S2: SEM 1 stepwise selection procedure. Supplementary material 3, Table S3: SEM 1 standardized direct, indirect and total size effects. Supplementary material 3, Figure S2: Initially hypothesized SEM 1. Supplementary material 4, Table S4: SEM 2 stepwise selection procedure. Supplementary material 4, Table S5: SEM 2 standardized direct, indirect and total size effects. Supplementary material 4, Figure S3: Initially hypothesized SEM 2. Supplementary material 5, Figure S4: Relationship between FH caterpillar development time, glucosinolate concentrations and flower number. Supplementary material 6, Table S6: GLMM/GAMM results for the effect of RH and FH on insect herbivore abundance, pollinator visitation and FH caterpillar parasitism rate.Plants are attacked by multiple herbivores, and depend on a precise regulation of responses to cope with a wide range of antagonists. Simultaneous herbivory can occur in different plant compartments, which may pose a serious threat to plant growth and reproduction. In particular, plants often face co-occurring root and floral herbivory, but few studies have focused on such interactions. Here, we investigated in the field the combined density-dependent effects of rootchewing cebrionid beetle larvae and flower-chewing pierid caterpillars on the fitness and defense of a semiarid Brassicaceae herb. We found that the fitness impact of both herbivore groups was independent and density-dependent. Increasing root herbivore density non-significantly reduced plant fitness, while the relationship between increasing floral herbivore density and the reduction they caused in both seed number and seedling emergence was non-linear. The plant defensive response was non-additive with regard to the different densities of root and floral herbivores; high floral herbivore density provoked compensatory investment in reproduction, and this tolerance response was combined with aboveground chemical defense induction when also root herbivore density was high. Plants may thus prioritize specific trait combinations in response to varying combined belowand aboveground herbivore densities to minimize negative impacts on fitness.Spanish Government, grant number CGL2011-24840Fellowship (BES-2012-059576

Towards a full understanding of Deception Island's magmatic plumbing system: An interdisciplinary approach

27th IUGG General Assembly 2019 in Montreal, Canada, 8-18 july 2019Deception Island (South Shetland Islands) is one of the most activevolcanoes in Antarctica, with more than 20 explosive eruptive events registered over the past two centuries. Recent eruptions (1967, 1969, and 1970) and the volcanic unrest episodes that happened in 1992, 1999, and 2014¿2015 demonstrate that the occurrence of future volcanic activity is a valid and pressing concern for scientists, military personnel, and tourists, that are visiting or working on or near the island. We present a unifying evolutionary model of the magmatic system beneath Deception Islandby integrating new petrologic and geochemical results with an exhaustive database of previous studies in the region. Our results reveal the existence of a complex plumbing system composed of several shallow magma chambers (¿ 10 km) fed by magmas raised directly from the mantle, or from a magma accumulation zone located at the crust-mantle boundary (15¿20 km depth). Understanding the current state of the island¿s magmatic system, and its potential evolution in the future, is fundamental to increase the effectiveness of interpreting monitoring data during volcanic unrest periods and hence, for future eruption forecasting.This research was supported by the MICINN grants RECALDEC (CTM2009-05919-E/ANT) and PEVOLDEC (CTM2011-13578-E/ANT), and the POSVOLDEC project (CTM2016-79617-P)(AEI/FEDER, UE). A.G. is grateful for her Ramón y Cajal contract (RYC-2012-11024). Analyses of stable isotopes were funded by the grant Programa Propio I (Usal-2014) through A.M.A-V

Deciphering the evolution of Deception Island’s magmatic system

Deception Island (South Shetland Islands) is one of the most active volcanoes in Antarctica, with more than 20 explosive eruptive events registered over the past two centuries. Recent eruptions (1967, 1969, and 1970) and the volcanic unrest episodes that happened in 1992, 1999, and 2014–2015 demonstrate that the occurrence of future volcanic activity is a valid and pressing concern for scientists, technical and logistic personnel, and tourists, that are visiting or working on or near the island. We present a unifying evolutionary model of the magmatic system beneath Deception Island by integrating new petrologic and geochemical results with an exhaustive database of previous studies in the region. Our results reveal the existence of a complex plumbing system composed of several shallow magma chambers (≤10 km depth) fed by magmas raised directly from the mantle, or from a magma accumulation zone located at the crust-mantle boundary (15–20 km depth). Understanding the current state of the island’s magmatic system, and its potential evolution in the future, is fundamental to increase the effectiveness of interpreting monitoring data during volcanic unrest periods and hence, for future eruption forecasting. © 2019, The Author(s).This research was supported by the MICINN grants RECALDEC (CTM2009–05919-E/ANT) and PEVOLDEC (CTM2011–13578-E/ANT), and the POSVOLDEC(CTM2016–79617-P)(AEI/FEDER, UE) and VOLCLIMA(CGL2015–72629-EXP)(AEI) projects. A.G. is grateful for her Ramón y Cajal contract (RYC-2012–11024). Analyses of stable isotopes were funded by the grant Programa Propio I (Usal-2014) through A.M.A-V.Peer reviewe

The magmatic system under Deception Island. What do the stable isotopes and noble gases decipher about it

Volatiles play a key role during the evolution of worldwide volcanoes. Analytical results of stable isotopes (deuterium, oxygen and noble gases) trapped in the volcanic products erupted at the active volcano of Deception Island (Antarctica) are allowing to advance the knowledge of (i) the magmatic and volcanic processes at depth of any monogenetic volcano in general; and (ii) the genesis, evolution and final eruptive events at Deception Island, in particular. This island is one of the most active volcanoes in Antarctica, with more than twenty explosive eruptions documented over the past two centuries. Therefore, the geochemistry of stable isotopes ¿integrated within the entire petrologic picture¿ from the magma source to the volcanic sink under Deception Island, reveals essential to increase the effectiveness of reacting against volcanic unrest periods and hence of future eruption forecast. This research was partially funded by the POSVOLDEC project (CTM2016¿79617¿P) (AEI/FEDER¿UE).Peer Reviewe

Deciphering Deception Island's magma plumbing system: An interdisciplinary approach

The XIII International Symposium on Antarctic Earth Science in Korea, 22-26 July 2019Deception Island (South Shetland Islands) is one of the most active volcanoes in Antarctica, with more than 20 explosive eruptive events registered over the past two centuries. Recent eruptions (1967, 1969, and 1970) and the volcanic unrest episodes that happened in 1992, 1999, and 2014¿ 2015 demonstrate that the occurrence of future volcanic activity is a valid and pressing concern for scientists, technical and logistic personnel, and tourists, that are visiting or working on or near the island. Understanding the current state of the island¿s magmatic system, and its potential evolution in the future, is fundamental to increase the effectiveness of interpreting monitoring data during volcanic unrest periods and hence, for future eruption forecasting. We present here a unifying evolutionary model of the magmatic system beneath Deception Island by integrating new petrologic and geochemical results with an exhaustive database of previous studies in the region. The results obtained reveal the existence of a complex plumbing system composed of several shallow magma chambers (¿ 10 km depth) fed by magmas raised directly from the mantle, or from a magma accumulation zone located at the crust-mantle boundary (15¿20 km depth). Our conclusions reinforce the perception of Deception Island as a very active and candidate volcano for a new eruption in the near future.This research was supported by the POSVOLDEC(CTM2016-79617-P)(AEI/FEDER, UE) research projec