Realización del proceso técnico y narrativo del montaje del videoclip Odisea espacial del grupo de rock psicodélico Nave Nebulosa.

[ES] Este trabajo trata de la realización de la edición y el montaje del videoclip ¿Odisea Espacial¿ del grupo de rock psicodélico Nave Nebulosa. Se habla del diseño tanto técnico como narrativo del montaje y se detalla el proceso del mismo, desde la visualización del material hasta la exportación. Se trata cada uno de los puntos fundamentales, como la organización del material filmado, la creación del proyecto y la secuencia, el uso de los diferentes códecs y formatos, un primer montaje o rough cut, un segundo montaje más fino, etc. A modo de introducción y con la finalidad de contextualizar el trabajo, se comenta brevemente la historia del videoclip y más concretamente del estilo rock psicodélico. También se habla de cómo fue el proceso de grabación del videoclip, tanto de la dirección de fotografía como de la dirección de arte. Para terminar, se realizará una corrección de color y una titulación para darle así un mejor acabado al trabajo y conseguir un resultado óptimo.[EN] This work deals with the editing and montage of the video clip "Space Odyssey" by the psychedelic rock group Nave Nebulosa. Both the technical and narrative design of the assembly is discussed and its process is detailed, from the visualization of the material to the export. Each of the fundamental points is covered, such as the organization of the filmed material, the creation of the project and the sequence, the use of the different codecs and formats, a first cut or rough cut, a second finer cut, etc. By way of introduction and in order to contextualize the work, the history of the video clip and more specifically of the psychedelic rock style are briefly discussed. They also talk about how the video clip recording process was, both the photography direction and the art direction. Finally, a color correction and a titration will be carried out.Barranco Tomás, M. (2021). Realización del proceso técnico y narrativo del montaje del videoclip Odisea espacial del grupo de rock psicodélico Nave Nebulosa. Universitat Politècnica de València. http://hdl.handle.net/10251/175460TFG

Clonal chromosomal mosaicism and loss of chromosome Y in elderly men increase vulnerability for SARS-CoV-2

The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) had an estimated overall case fatality ratio of 1.38% (pre-vaccination), being 53% higher in males and increasing exponentially with age. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, we found 133 cases (1.42%) with detectable clonal mosaicism for chromosome alterations (mCA) and 226 males (5.08%) with acquired loss of chromosome Y (LOY). Individuals with clonal mosaic events (mCA and/or LOY) showed a 54% increase in the risk of COVID-19 lethality. LOY is associated with transcriptomic biomarkers of immune dysfunction, pro-coagulation activity and cardiovascular risk. Interferon-induced genes involved in the initial immune response to SARS-CoV-2 are also down-regulated in LOY. Thus, mCA and LOY underlie at least part of the sex-biased severity and mortality of COVID-19 in aging patients. Given its potential therapeutic and prognostic relevance, evaluation of clonal mosaicism should be implemented as biomarker of COVID-19 severity in elderly people. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, individuals with clonal mosaic events (clonal mosaicism for chromosome alterations and/or loss of chromosome Y) showed an increased risk of COVID-19 lethality

Realización del videoclip de la canción "Rolling Down" de Nicky Shardlow

[ES] El presente trabajo incluye la realización del videoclip de la canción Rolling Down de Nicky Shardlow. El objetivo principal es crear un producto audiovisual profesional en forma de videoclip. La canción original tiene un tono triste y melancólico y el tratamiento visual debía seguir una estética adecuada para ilustrarlo. El modo de representación escogido fue de estilo narrativo, con un vínculo en forma de adaptación, es decir el videoclip desarrolla una historia independiente pero relacionada con los sentimientos y conceptos básicos de la canción. El video describe un episodio en la vida de la protagonista principal, Alma, que representa un caso de teen angst y expresa un rango de emociones que van evolucionando a lo largo de la historia, incluyendo desgana, ansiedad, rabia, desesperación, dolor, huida, lucha, esperanza y finalmente resignación. Las decisiones de dirección, el simbolismo, la fotografía y la interpretación se encaminaron a dotar al video de cierto dramatismo. El trabajo analiza el proyecto creativo incluyendo la preproduccio¿n, la producción y la postproduccio¿n tanto en la parte técnica como artística. Las materias analizadas incluyen el análisis musical y lírico de la canción, el guión literario y técnico, la fotografía, la edición, el etalonaje y los efectos.[EN] This work includes the realization of the music video of the song Rolling Down by Nicky Shardlow. The main objective is to create a professional audiovisual product in the format of a music video. The original song has a sad and melancholic tone and the visual treatment should follow a proper aesthetic to illustrate it. The mode of representation chosen was narrative style, with a link in the form of adaptation, that is, the music video develops an independent story but related to the feelings and basic concepts of the song. The video describes an episode in the life of the main protagonist, Alma, which represents a case of teen angst and expresses a range of emotions that evolve throughout history, including reluctance, anxiety, anger, despair, pain, fight, hope and finally resignation. The decisions of direction, symbolism, photography and interpretation were aimed at providing the video with a certain drama. The work analyzes the creative project including pre-production, production and post-production both in the technical and artistic part. The subjects analyzed include the musical and lyrical analysis of the song, the literary and technical script, photography, editing, grading and effects.Barranco Tomás, M. (2019). Realización del videoclip de la canción "Rolling Down" de Nicky Shardlow. Universitat Politècnica de València. http://hdl.handle.net/10251/128830TFG

Ejemplos de unidades didácticas en Educación Física para la Educación Secundaria Obligatoria

El objetivo es crear hábitos de Educación Física en conexión con valores socioculturales dentro de la E.S.O. La obra consta de varios ejemplos de unidades didácticas que se distribuyen teniendo en cuenta los objetivos, los contenidos, y las orientaciones de evaluación. El contenido de dichas unidades se organiza en juegos y deportes populares, la educación física para la salud, los juegos y deportes alternativos, la resistencia, la condición física y la salud. Concluye con un epígrafe dedicado a la alimentación, control de peso y ejercicio..MadridES

Galectin-1 is a novel therapeutic target and diagnostic/prognostic biomarker for pancreatic cancer

Trabajo presentado en la XVI Reunión de la Asociación Española de Pancreatología, celebrada en Bilbao (España), del 19 al 21 de septiembre de 2019Background: Pancreatic ductal adenocarcinoma (PDA) is one of the most lethal tumor types, with extremely low survival rates due to late diagnosis and resistance to standard therapies. Identification of new molecular targets and biomarkers is therefore an urgent need to improve this pessimistic scenario. Here we analyze the potential use of galectin-1 (Gal1), a glycan-binding protein that is highly overexpressed in PDA stroma, as therapeutic target and biomarker for PDA. Methods: To study the potential use of Gal1 as therapeutic target, effects of Gal1 inhibition has been tested using genetically engineered mouse models (Gal1 knockout mice and a KRas-driven mouse model of PDA) and a human cell-based system. To analyze whether Gal1 can be used as PDA biomarker, its levels were determined by ELISA in plasma from healthy controls and patients diagnosed with PDA, using three independent cohorts Results: Both in mouse models and in a human setting, inhibition of Gal1 leads to impaired tumor formation and metastasis, through modulation of multiple events including proliferation, angiogenesis and immune response. In addition, we found that plasma Gal-1 levels were significantly increased in PDA patients as compared to controls and that high levels of Gal-1 were associated with lower survival in patients with non-resected tumors. Conclusions: Gal1 plays a key role in PDA development and progression, highlighting the high therapeutic value of Gal1 inhibition in PDA treatment. Moreover, detection of Gal1 circulating levels shows a strong potential to be used as a novel biomarker for detection and prognostics of PDA patients

GWAS and meta-analysis identifies 49 genetic variants underlying critical COVID-19

Data availability: Downloadable summary data are available through the GenOMICC data site (https://genomicc.org/data). Summary statistics are available, but without the 23andMe summary statistics, except for the 10,000 most significant hits, for which full summary statistics are available. The full GWAS summary statistics for the 23andMe discovery dataset will be made available through 23andMe to qualified researchers under an agreement with 23andMe that protects the privacy of the 23andMe participants. For further information and to apply for access to the data, see the 23andMe website (https://research.23andMe.com/dataset-access/). All individual-level genotype and whole-genome sequencing data (for both academic and commercial uses) can be accessed through the UKRI/HDR UK Outbreak Data Analysis Platform (https://odap.ac.uk). A restricted dataset for a subset of GenOMICC participants is also available through the Genomics England data service. Monocyte RNA-seq data are available under the title ‘Monocyte gene expression data’ within the Oxford University Research Archives (https://doi.org/10.5287/ora-ko7q2nq66). Sequencing data will be made freely available to organizations and researchers to conduct research in accordance with the UK Policy Framework for Health and Social Care Research through a data access agreement. Sequencing data have been deposited at the European Genome–Phenome Archive (EGA), which is hosted by the EBI and the CRG, under accession number EGAS00001007111.Extended data figures and tables are available online at https://www.nature.com/articles/s41586-023-06034-3#Sec21 .Supplementary information is available online at https://www.nature.com/articles/s41586-023-06034-3#Sec22 .Code availability: Code to calculate the imputation of P values on the basis of SNPs in linkage disequilibrium is available at GitHub (https://github.com/baillielab/GenOMICC_GWAS).Acknowledgements: We thank the members of the Banco Nacional de ADN and the GRA@CE cohort group; and the research participants and employees of 23andMe for making this work possible. A full list of contributors who have provided data that were collated in the HGI project, including previous iterations, is available online (https://www.covid19hg.org/acknowledgements).Change history: 11 July 2023: A Correction to this paper has been published at: https://doi.org/10.1038/s41586-023-06383-z. -- In the version of this article initially published, the name of Ana Margarita Baldión-Elorza, of the SCOURGE Consortium, appeared incorrectly (as Ana María Baldion) and has now been amended in the HTML and PDF versions of the article.Copyright © The Author(s) 2023, Critical illness in COVID-19 is an extreme and clinically homogeneous disease phenotype that we have previously shown1 to be highly efficient for discovery of genetic associations2. Despite the advanced stage of illness at presentation, we have shown that host genetics in patients who are critically ill with COVID-19 can identify immunomodulatory therapies with strong beneficial effects in this group3. Here we analyse 24,202 cases of COVID-19 with critical illness comprising a combination of microarray genotype and whole-genome sequencing data from cases of critical illness in the international GenOMICC (11,440 cases) study, combined with other studies recruiting hospitalized patients with a strong focus on severe and critical disease: ISARIC4C (676 cases) and the SCOURGE consortium (5,934 cases). To put these results in the context of existing work, we conduct a meta-analysis of the new GenOMICC genome-wide association study (GWAS) results with previously published data. We find 49 genome-wide significant associations, of which 16 have not been reported previously. To investigate the therapeutic implications of these findings, we infer the structural consequences of protein-coding variants, and combine our GWAS results with gene expression data using a monocyte transcriptome-wide association study (TWAS) model, as well as gene and protein expression using Mendelian randomization. We identify potentially druggable targets in multiple systems, including inflammatory signalling (JAK1), monocyte–macrophage activation and endothelial permeability (PDE4A), immunometabolism (SLC2A5 and AK5), and host factors required for viral entry and replication (TMPRSS2 and RAB2A).GenOMICC was funded by Sepsis Research (the Fiona Elizabeth Agnew Trust), the Intensive Care Society, a Wellcome Trust Senior Research Fellowship (to J.K.B., 223164/Z/21/Z), the Department of Health and Social Care (DHSC), Illumina, LifeArc, the Medical Research Council, UKRI, a BBSRC Institute Program Support Grant to the Roslin Institute (BBS/E/D/20002172, BBS/E/D/10002070 and BBS/E/D/30002275) and UKRI grants MC_PC_20004, MC_PC_19025, MC_PC_1905 and MRNO2995X/1. A.D.B. acknowledges funding from the Wellcome PhD training fellowship for clinicians (204979/Z/16/Z), the Edinburgh Clinical Academic Track (ECAT) programme. This research is supported in part by the Data and Connectivity National Core Study, led by Health Data Research UK in partnership with the Office for National Statistics and funded by UK Research and Innovation (grant MC_PC_20029). Laboratory work was funded by a Wellcome Intermediate Clinical Fellowship to B.F. (201488/Z/16/Z). We acknowledge the staff at NHS Digital, Public Health England and the Intensive Care National Audit and Research Centre who provided clinical data on the participants; and the National Institute for Healthcare Research Clinical Research Network (NIHR CRN) and the Chief Scientist’s Office (Scotland), who facilitate recruitment into research studies in NHS hospitals, and to the global ISARIC and InFACT consortia. GenOMICC genotype controls were obtained using UK Biobank Resource under project 788 funded by Roslin Institute Strategic Programme Grants from the BBSRC (BBS/E/D/10002070 and BBS/E/D/30002275) and Health Data Research UK (HDR-9004 and HDR-9003). UK Biobank data were used in the GSMR analyses presented here under project 66982. The UK Biobank was established by the Wellcome Trust medical charity, Medical Research Council, Department of Health, Scottish Government and the Northwest Regional Development Agency. It has also had funding from the Welsh Assembly Government, British Heart Foundation and Diabetes UK. The work of L.K. was supported by an RCUK Innovation Fellowship from the National Productivity Investment Fund (MR/R026408/1). J.Y. is supported by the Westlake Education Foundation. SCOURGE is funded by the Instituto de Salud Carlos III (COV20_00622 to A.C., PI20/00876 to C.F.), European Union (ERDF) ‘A way of making Europe’, Fundación Amancio Ortega, Banco de Santander (to A.C.), Cabildo Insular de Tenerife (CGIEU0000219140 ‘Apuestas científicas del ITER para colaborar en la lucha contra la COVID-19’ to C.F.) and Fundación Canaria Instituto de Investigación Sanitaria de Canarias (PIFIISC20/57 to C.F.). We also acknowledge the contribution of the Centro National de Genotipado (CEGEN) and Centro de Supercomputación de Galicia (CESGA) for funding this project by providing supercomputing infrastructures. A.D.L. is a recipient of fellowships from the National Council for Scientific and Technological Development (CNPq)-Brazil (309173/2019-1 and 201527/2020-0)