Proteins of developing, mature and germinating seeds of phaseolus vulgaris

A number of methods have been used to extract, purify and characterise the major storage proteins of dry seeds of Phaseolus vulgaris L. cv. 'Seafarer'. Four fractions, which together account for the majority of the proteins solubilised by alkaline salt extractants and separated by pulyacrylamide gel electrophoresis, ' are described. The predominant protein, accounting for about 60% of the total protein content of the seed, is a vicilin-like protein, and is composed of two subunits, with molecular weights 50,000 and 47,000. A second protein fraction, prepared by zonal isoelectric precipitation, is a legumin-like protein; a third fraction was found to agglutinate erythrocytes. Amino acid compositions are presented for these protein fractions and their identity with storage proteins prepared in previous studies is discussed. The fourth fraction was not characterised. The protein content of protein bodies prepared by two methods was compared; the method of preparation was found to have a significant effect on the apparent distribution of both storage proteins and trypsin inhibitor activity. Variations in the distribution of storage proteins within the cotyledon are also described. The formation of storage protein during seed development and its subsequent mobilisation during seed germination was followed by SDS gel electrophoresis. Changes in proteolytic activity during germination were determined and attempts were made to identify the in vivo location of storage protein hydrolysis and the possible effect of endogenous inhibitors on this process

TM digital image products for applications

Computer compatible tapes (CCTs) of LANDSAT 4 thematic mapper (TM) digital image products are compared and reviewed. The following tape formats are discussed: (1) raw band-sequential data (CCT-BT); (2) calibrated data (CCT-AT); and (3) geometrically resampled data (CCT-PT). Each format represents different steps in the process of producing fully corrected TM data. The CCT-BT images are uncorrected radiometrically or geometrically, CCT-AT data are radiometrically calibrated, and CCT-PT images are both radiometrically and geometrically corrected

TM digital image products for applications

The image characteristics of digital data generated by LANDSAT 4 thematic mapper (TM) are discussed. Digital data from the TM resides in tape files at various stages of image processing. Within each image data file, the image lines are blocked by a factor of either 5 for a computer compatible tape CCT-BT, or 4 for a CCT-AT and CCT-PT; in each format, the image file has a different format. Nominal geometric corrections which provide proper geodetic relationships between different parts of the image are available only for the CCT-PT. It is concluded that detector 3 of band 5 on the TM does not respond; this channel of data needs replacement. The empty bin phenomenon in CCT-AT images results from integer truncations of mixed-mode arithmetric operations

Adaptation of Forage Species to Drought

Variability in rainfall is the single greatest cause of variation in forage production for a given site. Current climate scenarios predict future annual rainfall to decrease at some geographic regions. The intensity of future rainfall is also predicted to increase at other regions, with the expectation of greater variability in soil moisture. The adaptation of forage species to drought is an issue that is likely to remain with us into the future. Precise definitions are critical to water relations work, and imprecise use of terms has complicated comparisons of some studies on plant response to drought. Drought is a purely relative term, being abnormally low rainfall. Its duration and intensity will vary between locations. The intensity of drought is measured as water potential (-MPa) (relatively difficult), or water deficit (mm) (well suited to modeling) or soil water content (g/g, cm3/cm3, %). None of these measurements has a linear effect on plants. Drought resistance is a virtually meaningless term. Plant water reserves are trivial compared to the demand from the environment, and plants are virtually incapable of resisting drought. Forage plants do vary in tolerance to the intensity and duration of water deficit. Definition is further complicated by the scale of reference; at the plant-scale the plant might tolerate a level of water deficit, however tissues such as primordial are protected and do not encounter stress. At the tissue-scale certain cell components might be protected by osmotic adjustment, which allows some water loss by the plant but maintains turgor and some plant function

Superconducting and normal-state properties of the noncentrosymmetric superconductor Re6Zr

We systematically investigate the normal and superconducting properties of non-centrosymmetric Re$_{6}$Zr using magnetization, heat capacity, and electrical resistivity measurements. Resistivity measurements indicate Re$_{6}$Zr has poor metallic behavior and is dominated by disorder. Re$_6$Zr undergoes a superconducting transition at $T_{\mathrm{c}} = \left(6.75\pm0.05\right)$ K. Magnetization measurements give a lower critical field, $\mu_{0}H_{\mathrm{c1}} = \left(10.3 \pm 0.1\right)$ mT. The Werthamer-Helfand-Hohenberg model is used to approximate the upper critical field $\mu_{0}H_{\mathrm{c2}} = \left(11.2 \pm 0.2\right)$ T which is close to the Pauli limiting field of 12.35 T and which could indicate singlet-triplet mixing. However, low-temperature specific-heat data suggest that Re$_{6}$Zr is an isotropic, fully gapped s-wave superconductor with enhanced electron-phonon coupling. Unusual flux pinning resulting in a peak effect is observed in the magnetization data, indicating an unconventional vortex state.Comment: 11 pages, 7 figures, 2 table

Probing the superconducting ground state of the noncentrosymmetric superconductors CaTSi3 (T = Ir, Pt) using muon-spin relaxation and rotation

The superconducting properties of CaTSi3 (where T = Pt and Ir) have been investigated using muon spectroscopy. Our muon-spin relaxation results suggest that in both these superconductors time-reversal symmetry is preserved, while muon-spin rotation data show that the temperature dependence of the superfluid density is consistent with an isotropic s-wave gap. The magnetic penetration depths and upper critical fields determined from our transverse-field muon-spin rotation spectra are found to be 448(6) and 170(6) nm, and 3800(500) and 1700(300) G, for CaPtSi3 and CaIrSi3 respectively. The superconducting coherence lengths of the two materials have also been determined and are 29(2) nm for CaPtSi3 and 44(4) nm for CaIrSi3.Comment: 6 pages, 7 figure

Time-reversal symmetry breaking in noncentrosymmetric superconductor Re6Hf:further evidence for unconventional behaviour in the alpha-Mn family of materials

The discovery of new families of unconventional superconductors is important both experimentally and theoretically, especially if it challenges current models and thinking. By using muon spin relaxation in zero-field, time-reversal symmetry breaking has been observed in Re6Hf. Moreover, the temperature dependence of the superfluid density exhibits s-wave superconductivity with an enhanced electron-phonon coupling. This, coupled with the results from isostructural Re6Zr, shows that the Re6X family are indeed a new and important group of unconventional superconductors.Comment: 5 pages, 2 figures Accepted Physical Review B, Rapid Communicatio

Athlete learning in Olympic sport

High-performance sport impacts athletes beyond the physical. Coaches and coaching practice are particularly influential in shaping this learning and development. This article examines the learning identified through an inductive content analysis of eight former Olympic athletes’ career narratives. Three phases of learning could be identified across the cohort: ‘Growing into high-performance sport’, ‘Making sense of high-performance sport’, and ‘(Re)shaping high-performance sport’. A cultural perspective of learning, in particular the metaphor of ‘becoming’, is employed to interpret the Olympians’ learning experiences. The findings of this research indicate that athlete learning is bound by particular high-performance sporting contexts and career phases, yet impacted by the athletes’ individual backgrounds and dispositions. Further, data indicate that athletes’ personal development reflexively intertwines with athletic performance and performance enhancement. Implications for coaches are to: (1) involve athletes in co-constructing their sporting cultures and training contexts; and (2) provide possibilities and support for athletes to develop personally